Enzymes

• DpnI – R0176S, NEB
• EcoRI – R0101S, NEB
• SpeI – R0133S, NEB
• PstI – R0140S, NEB
• T4 Ligase – M0202, NEB
• Q5TM High fidelity DNA polymerase ref: M0491S - NEB
• Takara ExTaqTM Hot start Version. REF: RR006A - Clonetech

Primes and oligos

Primes were ordered from http://www.lifetechnologies.com

Sequencing

Sequencing - We used GATC sequencing.

Kits

• Miniprep - Plasmid purification.

  NucleoSpin® Plasmid, Ref: 740588.25 - Macherey Nagel (MN)

  Wizard® Plus SV Minipreps DNA Purification System + Vacuum Adapters, Catalog #A1470 - Promega

• PCR and Gel clean up

  Macherey Nagel (MN) - NucleoSpin® Gel and PCR Clean-up. Ref 740609.50

• Midori Green Advanced DNA stain Cat.No MG03 – Nippon genetics Europe GmbH