Team:BostonU/Parts

From 2014.igem.org

(Difference between revisions)
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<table id="promoters" style="border:1px solid black; border-collapse:collapse">
<table id="promoters" style="border:1px solid black; border-collapse:collapse">
   <tr>
   <tr>
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     <th style="border:1px solid black"><font color="#B22222">Part Name</font></th>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;Part Name&nbsp;</font></th>
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     <th style="border:1px solid black"><font color="#B22222">Part Type</font></th>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;Part Type&nbsp;</font></th>
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     <th style="border:1px solid black"><font color="#B22222">MoClo Level</font></th>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;MoClo Level&nbsp;</font></th>
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     <th style="border:1px solid black"><font color="#B22222">Antibiotic Resistance</font></th>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;Antibiotic Resistance&nbsp;</font></th>
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     <th style="border:1px solid black"><font color="#B22222">BBa Name</font></th></h8>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;BBa Name&nbsp;</font></th></h8>
   </tr>
   </tr>
   <tr>
   <tr>
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<table id="devices" style="border:1px solid black; border-collapse:collapse">
<table id="devices" style="border:1px solid black; border-collapse:collapse">
   <tr>
   <tr>
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     <th style="border:1px solid black"><font color="#B22222">Device Name</font></th>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;Device Name&nbsp;</font></th>
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     <th style="border:1px solid black"><font color="#B22222">Device Description</font></th>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;Device Description&nbsp;</font></th>
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     <th style="border:1px solid black"><font color="#B22222">Component Parts</font></th>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;Component Parts&nbsp;</font></th>
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     <th style="border:1px solid black"><font color="#B22222">Destination Vector</font></th>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;Destination Vector&nbsp;</font></th>
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     <th style="border:1px solid black"><font color="#B22222">MoClo Level</font></th>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;MoClo Level&nbsp;</font></th>
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     <th style="border:1px solid black"><font color="#B22222">Antibiotic Resistance</font></th>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;Antibiotic Resistance&nbsp;</font></th>
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     <th style="border:1px solid black"><font color="#B22222">BBa Name</font></th></h8>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;BBa Name&nbsp;</font></th></h8>
   </tr>
   </tr>
   <tr>
   <tr>
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     <td style="border:1px solid black;">pTet-I13453RFP</td>
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     <td style="border:1px solid black;">&nbsp;pTet-I13453RFP&nbsp;</td>
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     <td style="border:1px solid black;">pInducible</td>
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     <td style="border:1px solid black;">&nbsp;pInducible&nbsp;</td>
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     <td style="border:1px solid black;">pTet-I13453_AB+BCD2_BC+E1010m_CD+B0015_DE</td>
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     <td style="border:1px solid black;">&nbsp;pTet-I13453_AB+BCD2_BC+E1010m_CD+B0015_DE&nbsp;</td>
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     <td style="border:1px solid black;">DVL1_AE</td>
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     <td style="border:1px solid black;">&nbsp;DVL1_AE&nbsp;</td>
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     <td style="border:1px solid black;">Level 1</td>
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     <td style="border:1px solid black;">&nbsp;Level 1&nbsp;</td>
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     <td style="border:1px solid black;">Kanamycin</td>
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     <td style="border:1px solid black;">&nbsp;Kanamycin&nbsp;</td>
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     <td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1401009">BBa_K1401009</a></td>
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     <td style="border:1px solid black;">&nbsp;<a href="http://parts.igem.org/Part:BBa_K1401009">BBa_K1401009</a>&nbsp;</td>
   </tr>
   </tr>
   <tr>
   <tr>
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     <td style="border:1px solid black;">I13453-pTetRFP</td>
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     <td style="border:1px solid black;">&nbsp;I13453-pTetRFP&nbsp;</td>
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     <td style="border:1px solid black;">pInducible</td>
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     <td style="border:1px solid black;">&nbsp;pInducible&nbsp;</td>
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     <td style="border:1px solid black;">I13453-pTet_AB+BCD2_BC+C0040_CD+B0015_DE</td>
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     <td style="border:1px solid black;">&nbsp;I13453-pTet_AB+BCD2_BC+C0040_CD+B0015_DE&nbsp;</td>
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     <td style="border:1px solid black;">DVL1_AE</td>
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     <td style="border:1px solid black;">&nbsp;DVL1_AE&nbsp;</td>
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     <td style="border:1px solid black;">Level 1</td>
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     <td style="border:1px solid black;">&nbsp;Level 1&nbsp;</td>
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     <td style="border:1px solid black;">Kanamycin</td>
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     <td style="border:1px solid black;">&nbsp;Kanamycin&nbsp;</td>
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     <td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1401010">BBa_K1401010</a></td>
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     <td style="border:1px solid black;">&nbsp;<a href="http://parts.igem.org/Part:BBa_K1401010">BBa_K1401010</a>&nbsp;</td>
   </tr>
   </tr>
</table>
</table>
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<table id="devices" style="border:1px solid black; border-collapse:collapse">
<table id="devices" style="border:1px solid black; border-collapse:collapse">
   <tr>
   <tr>
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     <th style="border:1px solid black"><font color="#B22222">Device Name</font></th>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;Device Name&nbsp;</font></th>
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     <th style="border:1px solid black"><font color="#B22222">Device Description</font></th>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;Device Description&nbsp;</font></th>
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     <th style="border:1px solid black"><font color="#B22222">Component Parts</font></th>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;Component Parts&nbsp;</font></th>
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     <th style="border:1px solid black"><font color="#B22222">Destination Vector</font></th>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;Destination Vector&nbsp;</font></th>
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     <th style="border:1px solid black"><font color="#B22222">MoClo Level</font></th>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;MoClo Level&nbsp;</font></th>
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     <th style="border:1px solid black"><font color="#B22222">Antibiotic Resistance</font></th>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;Antibiotic Resistance&nbsp;</font></th>
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     <th style="border:1px solid black"><font color="#B22222">BBa Name</font></th></h8>
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     <th style="border:1px solid black"><font color="#B22222">&nbsp;BBa Name&nbsp;</font></th></h8>
   </tr>
   </tr>
   <tr>
   <tr>
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     <td style="border:1px solid black;">pI13B2Rm_FG</td>
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     <td style="border:1px solid black;">&nbsp;pI13B2Rm_FG&nbsp;</td>
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     <td style="border:1px solid black;">pRepressible</td>
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     <td style="border:1px solid black;">&nbsp;pRepressible&nbsp;</td>
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     <td style="border:1px solid black;">I13453_FB+BCD2_BC+E1010m_CD+B0015_DG</td>
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     <td style="border:1px solid black;">&nbsp;I13453_FB+BCD2_BC+E1010m_CD+B0015_DG&nbsp;</td>
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     <td style="border:1px solid black;">DVL1_FG</td>
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     <td style="border:1px solid black;">&nbsp;DVL1_FG&nbsp;</td>
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     <td style="border:1px solid black;">Level 1</td>
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     <td style="border:1px solid black;">&nbsp;Level 1&nbsp;</td>
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     <td style="border:1px solid black;">Kanamycin</td>
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     <td style="border:1px solid black;">&nbsp;Kanamycin&nbsp;</td>
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     <td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1401606">BBa_K1401606</a></td>
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     <td style="border:1px solid black;">&nbsp;<a href="http://parts.igem.org/Part:BBa_K1401606">BBa_K1401606</a>&nbsp;</td>
   </tr>
   </tr>
   <tr>
   <tr>
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     <td style="border:1px solid black;">pR10B2C40_EF</td>
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     <td style="border:1px solid black;">&nbsp;pR10B2C40_EF&nbsp;</td>
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     <td style="border:1px solid black;">pRepressible</td>
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     <td style="border:1px solid black;">&nbsp;pRepressible&nbsp;</td>
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     <td style="border:1px solid black;">R0010_EB+BCD2_BC+C0040_CD+B0015_DF</td>
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     <td style="border:1px solid black;">&nbsp;R0010_EB+BCD2_BC+C0040_CD+B0015_DF&nbsp;</td>
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     <td style="border:1px solid black;">DVL1_EF</td>
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     <td style="border:1px solid black;">&nbsp;DVL1_EF&nbsp;</td>
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     <td style="border:1px solid black;">Level 1</td>
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     <td style="border:1px solid black;">&nbsp;Level 1&nbsp;</td>
-
     <td style="border:1px solid black;">Kanamycin</td>
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     <td style="border:1px solid black;">&nbsp;Kanamycin&nbsp;</td>
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     <td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1401607">BBa_K1401607</a></td>
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     <td style="border:1px solid black;">&nbsp;<a href="http://parts.igem.org/Part:BBa_K1401607">BBa_K1401607</a>&nbsp;</td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td style="border:1px solid black;">pR10B2C40Y_EF</td>
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     <td style="border:1px solid black;">&nbsp;pR10B2C40Y_EF&nbsp;</td>
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     <td style="border:1px solid black;">pRepressible with Fusion Protein</td>
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     <td style="border:1px solid black;">&nbsp;pRepressible with Fusion Protein&nbsp;</td>
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     <td style="border:1px solid black;">R0010_EB+BCD2_BC+C0040_CI+E0030_ID+B0015_DF</td>
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     <td style="border:1px solid black;">&nbsp;R0010_EB+BCD2_BC+C0040_CI+E0030_ID+B0015_DF&nbsp;</td>
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     <td style="border:1px solid black;">DVL1_EF</td>
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     <td style="border:1px solid black;">&nbsp;DVL1_EF&nbsp;</td>
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     <td style="border:1px solid black;">Level 1</td>
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     <td style="border:1px solid black;">&nbsp;Level 1&nbsp;</td>
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     <td style="border:1px solid black;">Kanamycin</td>
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     <td style="border:1px solid black;">&nbsp;Kanamycin&nbsp;</td>
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     <td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1401608">BBa_K1401608</a></td>
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     <td style="border:1px solid black;">&nbsp;<a href="http://parts.igem.org/Part:BBa_K1401608">BBa_K1401608</a>&nbsp;</td>
   </tr>
   </tr>
   <tr>
   <tr>
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     <td style="border:1px solid black;">pR10B2Gm_EF</td>
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     <td style="border:1px solid black;">&nbsp;pR10B2Gm_EF&nbsp;</td>
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     <td style="border:1px solid black;">pRepressible</td>
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     <td style="border:1px solid black;">&nbsp;pRepressible&nbsp;</td>
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     <td style="border:1px solid black;">R0010_EB+BCD2_BC+E0040m_CD+B0015_DF</td>
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     <td style="border:1px solid black;">&nbsp;R0010_EB+BCD2_BC+E0040m_CD+B0015_DF&nbsp;</td>
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     <td style="border:1px solid black;">DVL1_EF</td>
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     <td style="border:1px solid black;">&nbsp;DVL1_EF&nbsp;</td>
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     <td style="border:1px solid black;">Level 1</td>
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     <td style="border:1px solid black;">&nbsp;Level 1&nbsp;</td>
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     <td style="border:1px solid black;">Kanamycin</td>
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     <td style="border:1px solid black;">&nbsp;Kanamycin&nbsp;</td>
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     <td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1401609">BBa_K1401609</a></td>
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     <td style="border:1px solid black;">&nbsp;<a href="http://parts.igem.org/Part:BBa_K1401609">BBa_K1401609</a>&nbsp;</td>
   </tr>
   </tr>
     <tr>
     <tr>
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     <td style="border:1px solid black;">pR10B2Y_EF</td>
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     <td style="border:1px solid black;">&nbsp;pR10B2Y_EF&nbsp;</td>
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     <td style="border:1px solid black;"> pRepressible </td>
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     <td style="border:1px solid black;"> &nbsp;pRepressible &nbsp;</td>
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     <td style="border:1px solid black;"> R0010_EB+BCD2_BC+E0030_CD+B0015_DF </td>
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     <td style="border:1px solid black;"> &nbsp;R0010_EB+BCD2_BC+E0030_CD+B0015_DF &nbsp;</td>
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     <td style="border:1px solid black;">DVL1_EF</td>
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     <td style="border:1px solid black;">&nbsp;DVL1_EF&nbsp;</td>
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     <td style="border:1px solid black;">Level 1</td>
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     <td style="border:1px solid black;">&nbsp;Level 1&nbsp;</td>
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     <td style="border:1px solid black;">Kanamycin</td>
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     <td style="border:1px solid black;">&nbsp;Kanamycin&nbsp;</td>
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     <td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1401610">BBa_K1401610</a></td>
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     <td style="border:1px solid black;">&nbsp;<a href="http://parts.igem.org/Part:BBa_K1401610">BBa_K1401610</a>&nbsp;</td>
   </tr>
   </tr>
   <tr>
   <tr>
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     <td style="border:1px solid black;">pR40B2Rm_FG</td>
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     <td style="border:1px solid black;">&nbsp;pR40B2Rm_FG&nbsp;</td>
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     <td style="border:1px solid black;"> pRepressible </td>
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     <td style="border:1px solid black;"> &nbsp;pRepressible &nbsp;</td>
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     <td style="border:1px solid black;">R0040_FB+BCD2_BC+E1010m_CD+B0015_DG</td>
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     <td style="border:1px solid black;">&nbsp;R0040_FB+BCD2_BC+E1010m_CD+B0015_DG&nbsp;</td>
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     <td style="border:1px solid black;">DVL1_FG</td>
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     <td style="border:1px solid black;">&nbsp;DVL1_FG&nbsp;</td>
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     <td style="border:1px solid black;">Level 1</td>
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     <td style="border:1px solid black;">&nbsp;Level 1&nbsp;</td>
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     <td style="border:1px solid black;">Kanamycin</td>
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     <td style="border:1px solid black;">&nbsp;Kanamycin&nbsp;</td>
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     <td style="border:1px solid black;"><a href="http://parts.igem.org/Part:BBa_K1401611">BBa_K1401611</a></td>
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     <td style="border:1px solid black;">&nbsp;<a href="http://parts.igem.org/Part:BBa_K1401611">BBa_K1401611</a>&nbsp;</td>
   </tr>
   </tr>
<tr>
<tr>

Revision as of 05:31, 17 October 2014



Parts Submitted

Parts submitted for Bronze Medal Consideration

 Part Name   Part Type   MoClo Level   Antibiotic Resistance   BBa Name 
 C0040_CI   Repressor Fusion Protein   Level 0   Chloramphenicol   BBa_K1401600 
 C0080_CI   Repressor Fusion Protein    Level 0   Chloramphenicol   BBa_K1401601 
 E0040m_ID   Reporter Fusion Protein   Level 0   Chloramphenicol   BBa_K1401602 
 E0030_ID   Repressor Fusion Protein   Level 0   Chloramphenicol   BBa_K1401603 
 pTet+pBAD   Tandem Promoter   Level 0   Chloramphenicol   BBa_K1401007 
 pBAD+pTet   Tandem Promoter   Level 0   Chloramphenicol   BBa_K1401008 

The parts in bold (pTet+pBAD and pBAD+pTet) are the ones submitted for Silver Medal consideration i.e. they have data on the registry pages.

Parts used to build and test Tandem Promoters

 Device Name   Device Description   Component Parts   Destination Vector   MoClo Level   Antibiotic Resistance   BBa Name 
 pTet-I13453RFP   pInducible   pTet-I13453_AB+BCD2_BC+E1010m_CD+B0015_DE   DVL1_AE   Level 1   Kanamycin   BBa_K1401009 
 I13453-pTetRFP   pInducible   I13453-pTet_AB+BCD2_BC+C0040_CD+B0015_DE   DVL1_AE   Level 1   Kanamycin   BBa_K1401010 

*Note: The pTet used in this experiment is not Bba_R0040.

Parts used to build and test Fusion Proteins

 Device Name   Device Description   Component Parts   Destination Vector   MoClo Level   Antibiotic Resistance   BBa Name 
 pI13B2Rm_FG   pRepressible   I13453_FB+BCD2_BC+E1010m_CD+B0015_DG   DVL1_FG   Level 1   Kanamycin   BBa_K1401606 
 pR10B2C40_EF   pRepressible   R0010_EB+BCD2_BC+C0040_CD+B0015_DF   DVL1_EF   Level 1   Kanamycin   BBa_K1401607 
 pR10B2C40Y_EF   pRepressible with Fusion Protein   R0010_EB+BCD2_BC+C0040_CI+E0030_ID+B0015_DF   DVL1_EF   Level 1   Kanamycin   BBa_K1401608 
 pR10B2Gm_EF   pRepressible   R0010_EB+BCD2_BC+E0040m_CD+B0015_DF   DVL1_EF   Level 1   Kanamycin   BBa_K1401609 
 pR10B2Y_EF   pRepressible    R0010_EB+BCD2_BC+E0030_CD+B0015_DF    DVL1_EF   Level 1   Kanamycin   BBa_K1401610 
 pR40B2Rm_FG   pRepressible    R0040_FB+BCD2_BC+E1010m_CD+B0015_DG   DVL1_FG   Level 1   Kanamycin   BBa_K1401611 
pR10B2C80_EF pRepressible R0010_EB+BCD2_BC+C0080_CD+B0015_DF DVL1_EF Level 1 Kanamycin BBa_K1401612
pR10B2C80Y_EF pRepressible with Fusion Protein R0010_EB+BCD2_BC+C0080_CI+E0030_ID+B0015_DF DVL1_EF Level 1 Kanamycin BBa_1401613
pR10B2C80Gm_EF pRepressible with Fusion Protein R0010_EB+BCD2_BC+C0080_CI+E0040m_ID+B0015_DF DVL1_EF Level 1 Kanamycin BBa_K1401614
pR10B2C40Gm_EF pRepressible with Fusion Protein R0010_EB+BCD2_BC+C0040_CI+E0040m_ID+B0015_DF DVL1_EF Level 1 Kanamycin BBa_K1401615
pJ00B2C12m_AE pConstitutive J23100_AB+BCD2_BC+C0012m_CD+B0015_DE DVL1_AE Level 1 Kanamycin BBa_K1401616







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