Team:SCU-China/Team

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<link rel="stylesheet" href="https://2014.igem.org/Team:SCU-China/bootstrap.min.css ?action=raw&amp;ctype=text/css" rel="stylesheet" type="text/css"/>
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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<br>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:SCU-China/Team&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:SCU-China/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=SCU-China"style="color:#000000"> Official Team Profile </a></td>
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            <li class="dropdown"><a href="#" class="dropdown-toggle" data-toggle="dropdown">Project <span class="caret"></span></a>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Background">Background</a></li>
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<a href="https://2014.igem.org/Team:SCU-China/Project"style="color:#000000"> Project</a></td>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Overview">Overview</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Description">Description</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Result">Result</a></li>
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<a href="https://2014.igem.org/Team:SCU-China/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:SCU-China/Modeling"style="color:#000000"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:SCU-China/Notebook"style="color:#000000"> Notebook</a></td>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Seventh">Presentation at Seventh Senior High School</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/UESTC">Visiting University of Electronic Science and Technology of China (UESTC)</a></li>
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<a href="https://2014.igem.org/Team:SCU-China/Safety"style=" color:#000000"> Safety </a></td>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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<li><a href="https://2014.igem.org/Team:SCU-China/Safety">Safety</a></li>
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<a href="https://2014.igem.org/Team:SCU-China/Attributions"style="color:#000000"> Attributions </a></td>
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<li><a href="https://2014.igem.org/Team:SCU-China/Parts">Parts</a></li>  
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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            <li><a href="https://2014.igem.org/Team:SCU-China/Attributions">Attributions</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Transmitter">Notebook of Transmitter</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Effector">Notebook of Effector</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Sensor">Notebook of Sensor</a></li>
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                <li class="divider"></li>
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                <li class="dropdown-header">Method</li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Prep">Bacterial Genomic DNA Prep</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Digestion">Digestion</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/GelExtraction">Gel Extraction </a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Linkage">Linkage</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/PCR">PCR</a></li>
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                <li><a href="https://2014.igem.org/Team:SCU-China/Plasmid">Plasmid Mini Prep</a></li>
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              </ul>
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<p>For constructing the Biobricks that we want to submit, we set up the PCR protocol and PCR system as bellows:</p><table class="table table-striped"> <caption>PCR system (For 50&#956;L)</caption> <thead><tr><th><p>Materials</p>
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</th><th><p>Volume</p>
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</th>  
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</tr></thead> <tbody><tr><td><p>Taq DNA polymerase</p>
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</td><td><p>0.5&#956;L</p>
</td>
</td>
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</tr><tr><td><p>Template</p>
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</td><td><p>0.5&#956;L</p>
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<tr><td > <h3> Team Information</h3></td>
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<td ></td >
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<td > <h3> Upload pictures of your team!</h3></td>
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<td width="45%"  valign="top">
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You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.</p>
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<p>
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You can look at what other teams did to get some inspiration! Here are a few examples:</p>
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<li><a href="https://2013.igem.org/Team:Evry/Team">2013 Evry</a></li>
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<li><a href="https://2013.igem.org/Team:Cornell/team">2013 Cornell</a></li>
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<li><a href="https://2013.igem.org/Team:UANL_Mty-Mexico/Team">2013 UANL Mty-Mexico</a></li>
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<li><a href="https://2013.igem.org/Team:Korea_U_Seoul/Team/Korea_University">2013 Korea U Seoul</a></li>
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</td>
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<td></td>
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<p> We provide a server where you can upload images for your wiki!</p>
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<p> Add pictures of your team members, advisors, instructors, lab, institution and everything you wish to show! </p>
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<a href="https://2014.igem.org/Special:Upload"><img src="" width="100px" height="100px">  </a> <a href="https://2014.igem.org/Special:Upload"><img src="" width="100px" height="100px">  </a>
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</a> <a href="https://2014.igem.org/Special:Upload"><img src="" width="100px" height="100px">  </a>
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<br>
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<a href="https://2014.igem.org/Special:Upload">Click here to upload! </a>
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</td>
</td>
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</tr><tr><td><p>Forward Primer</p>
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</td><td><p>1&#956;L</p>
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</td>
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</tr><tr><td><p>Reverse Primer</p>
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</td><td><p>1&#956;L</p>
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</td>
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</tr><tr><td><p>dNTPs (2.5mM)</p>
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</td><td><p>4&#956;L</p>
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</td>
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</tr><tr><td><p>10x Buffer (Mg2+ free)</p>
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</td><td><p>5&#956;L</p>
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</td>
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</tr><tr><td><p>MgCl2 (25mM)</p>
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</td><td><p>3&#956;L</p>
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</td>
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</tr><tr><td><p>Sterilized diluted water</p>
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</td><td><p>35&#956;L</p>
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</td>
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</tr></tbody>
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</table><p>Note: The content of template should be less than 500ng for the 50&#956;L system. For all of our experiments, the content of template of 0.5&#956;L DNA lower than 500ng.</p>
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<table class="table table-striped"> <caption>PCR protocol</caption><thead><tr><th><p>Temperature</p>
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</tr>
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</th><th><p>Times</p>
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</th>
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<tr><td > <h3> Tips for the Team Page </h3></td>
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</tr></thead> <tbody><tr><td><p>95&#8451;</p>
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<td ></td >
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</td><td><p>5min.</p>
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<td > </td>
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</td><th rowspan="3">30-35 Times</th>
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</tr><tr><td><p>95&#8451;</p>
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</td><td><p>30sec.</p>
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</td>
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</tr><tr><td><p>55-65&#8451;</p>
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<td width="45%"  valign="top">  
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</td><td><p>30sec.</p>
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<p>What should this page contain?</p>
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<ul>
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<li> Include pictures of your teammates, don’t forget instructors and advisors! </li>
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<li> You can add a small biography or a few words from each team member, tell us what you like, and what motivates to participate in iGEM. </li>
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<li>Take team pictures! Show us your school, your lab and little bit of your city.</li>
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<li>Remember that image galleries can help you showcase many pictures while saving space </li>
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</ul>
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</td>
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</tr><tr><td><p>72&#8451;</p>
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</td><td><p>1-2min.</p>
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</td>
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</tr><tr><td><p>72&#8451;</p>
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</td><td><p>10min.</p>
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</td>
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</tr></tbody>
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</table><p>Note: for finding the optimal annealing temperature, we have done a series of gradient of annealing temperature first. And as for the elongation time, Taq DNA polymerase could synthesize DNA as the speed of 1000-2000 nucleotides per minute.</p>
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  <p>Sichuan university</p>
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</div></div>

Revision as of 05:08, 17 October 2014

PCR Protocol

For constructing the Biobricks that we want to submit, we set up the PCR protocol and PCR system as bellows:

PCR system (For 50μL)

Materials

Volume

Taq DNA polymerase

0.5μL

Template

0.5μL

Forward Primer

1μL

Reverse Primer

1μL

dNTPs (2.5mM)

4μL

10x Buffer (Mg2+ free)

5μL

MgCl2 (25mM)

3μL

Sterilized diluted water

35μL

Note: The content of template should be less than 500ng for the 50μL system. For all of our experiments, the content of template of 0.5μL DNA lower than 500ng.

PCR protocol

Temperature

Times

95℃

5min.

30-35 Times

95℃

30sec.

55-65℃

30sec.

72℃

1-2min.

72℃

10min.

Note: for finding the optimal annealing temperature, we have done a series of gradient of annealing temperature first. And as for the elongation time, Taq DNA polymerase could synthesize DNA as the speed of 1000-2000 nucleotides per minute.

Sichuan university

Retrieved from "http://2014.igem.org/Team:SCU-China/Team"