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Team:BostonU/Collaborations
From 2014.igem.org
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They gave us two copies of the same construct, one in a chloramphenicol resistant backbone and the other in an ampicillin resistant backbone. The device has BclA-YFP, which is a cell surface targeted protein to express YFP on the cell surface. We also ran one of our own single color YFP controls, which is listed at "Internal YFP" as this is not targeted to the cell surface. It should be noted that this control is in a kanamycin backbone. | They gave us two copies of the same construct, one in a chloramphenicol resistant backbone and the other in an ampicillin resistant backbone. The device has BclA-YFP, which is a cell surface targeted protein to express YFP on the cell surface. We also ran one of our own single color YFP controls, which is listed at "Internal YFP" as this is not targeted to the cell surface. It should be noted that this control is in a kanamycin backbone. | ||
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| - | The results our shown below. For details on how we converted the arbitrary YFP units to MEFLs, please see the <a href="https://2014.igem.org/Team:BostonU/ | + | The results our shown below. For details on how we converted the arbitrary YFP units to MEFLs, please see the <a href="https://2014.igem.org/Team:BostonU/Software#flow">TASBE Tools</a> page for information about controls required to do that conversion. |
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<center><img src="https://static.igem.org/mediawiki/2014/0/0e/Bar_graphs_YFP.png" width="60%"></center> | <center><img src="https://static.igem.org/mediawiki/2014/0/0e/Bar_graphs_YFP.png" width="60%"></center> | ||
Revision as of 22:48, 15 October 2014
WPI-Worcester
| This year, the BU iGEM team collaborated with the WPI-Worcester iGEM team.
The collaboration became possible at the NEGEM meet up in June, where six New England iGEM teams presented their project ideas to the rest. During one of our small group discussions, a team member from WPI talked about how lucky we, the BU team, were to get access to a flow cytometer for single cell fluorescence measurement. Right then, the two teams decided to collaborate: we would test some of their constructs on our flow cytometer and they would view cells expressing our devices with their confocal microscope. They gave us two copies of the same construct, one in a chloramphenicol resistant backbone and the other in an ampicillin resistant backbone. The device has BclA-YFP, which is a cell surface targeted protein to express YFP on the cell surface. We also ran one of our own single color YFP controls, which is listed at "Internal YFP" as this is not targeted to the cell surface. It should be noted that this control is in a kanamycin backbone. The results our shown below. For details on how we converted the arbitrary YFP units to MEFLs, please see the TASBE Tools page for information about controls required to do that conversion.  |
Inter Lab Study Collaboration with Tufts iGEM and MIT iGEM teams
In August, researchers from the Tufts iGEM team contacted us and described that they had been having trouble transforming some parts, which were required for the Inter Lab Study , into cells. The parts that they needed were -
We located those parts, members from the Tufts team picked them up and successfully measured the required constructs for the study. During the 3rd NEGEM Meetup in October, MIT iGEM also expressed interest in taking part in the Inter Lab Study. We promptly streaked all required constructs on agar plates for them, which they could then use to make cultures and test on their Flow Cytometer. Our results for the study can be found on our Inter Lab Study page. |
