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Team:Calgary/OurTeam/Outreach/TelusSpark
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<h1>Telus Spark</h1> | <h1>Telus Spark</h1> | ||
<h2>DNAquiris anyone?</h2> | <h2>DNAquiris anyone?</h2> | ||
| - | <img src="https://static.igem.org/mediawiki/2014/d/d4/UCalgary2014DNAquiri.jpg" align="right" height=" | + | <img src="https://static.igem.org/mediawiki/2014/d/d4/UCalgary2014DNAquiri.jpg" align="right" height="35%" width="35%"> |
<p>For our first event in June, we used frozen-thawed strawberries and fresh pineapple juice not only to create a delicious daiquiri, but also to extract DNA within minutes in front of thirsty spectators. In order to do so, we used frozen strawberries and let them thaw at room temperature for roughly half an hour. Using a mallet we crushed them in a Ziploc bag, this lysed open the strawberry cells, making the DNA accessible for the extraction. Next we added fresh pineapple juice and let we let it incubate in a warm bath. An active protease in the pineapple juice, bromelain, denatures the histones and additional proteins holding the DNA tightly together. Now that there is free flowing DNA, we add the magic ingredient. Bacardi! One shot allows for the DNA to surface up and can be picked up using a toothpick.<p> | <p>For our first event in June, we used frozen-thawed strawberries and fresh pineapple juice not only to create a delicious daiquiri, but also to extract DNA within minutes in front of thirsty spectators. In order to do so, we used frozen strawberries and let them thaw at room temperature for roughly half an hour. Using a mallet we crushed them in a Ziploc bag, this lysed open the strawberry cells, making the DNA accessible for the extraction. Next we added fresh pineapple juice and let we let it incubate in a warm bath. An active protease in the pineapple juice, bromelain, denatures the histones and additional proteins holding the DNA tightly together. Now that there is free flowing DNA, we add the magic ingredient. Bacardi! One shot allows for the DNA to surface up and can be picked up using a toothpick.<p> | ||
<p>This sparked conversations regarding GMO foods and flavour enhancers like MSG, and the true science behind them. We got plenty of questions, like “Do strawberries have DNA?” and “Can GMOs be harmful?” While some answers were more complicated than other, we got a wonderful opportunity to debunk any myths surrounding such topics.<p> | <p>This sparked conversations regarding GMO foods and flavour enhancers like MSG, and the true science behind them. We got plenty of questions, like “Do strawberries have DNA?” and “Can GMOs be harmful?” While some answers were more complicated than other, we got a wonderful opportunity to debunk any myths surrounding such topics.<p> | ||
Revision as of 21:18, 15 October 2014
Telus Spark
DNAquiris anyone?
For our first event in June, we used frozen-thawed strawberries and fresh pineapple juice not only to create a delicious daiquiri, but also to extract DNA within minutes in front of thirsty spectators. In order to do so, we used frozen strawberries and let them thaw at room temperature for roughly half an hour. Using a mallet we crushed them in a Ziploc bag, this lysed open the strawberry cells, making the DNA accessible for the extraction. Next we added fresh pineapple juice and let we let it incubate in a warm bath. An active protease in the pineapple juice, bromelain, denatures the histones and additional proteins holding the DNA tightly together. Now that there is free flowing DNA, we add the magic ingredient. Bacardi! One shot allows for the DNA to surface up and can be picked up using a toothpick.
This sparked conversations regarding GMO foods and flavour enhancers like MSG, and the true science behind them. We got plenty of questions, like “Do strawberries have DNA?” and “Can GMOs be harmful?” While some answers were more complicated than other, we got a wonderful opportunity to debunk any myths surrounding such topics.
Bacteria Art
For our second event we let the imagination of Calgarians run wild with our Bacterial Art station. We prepared 4 different strains of E. coli expressing fluorescent proteins (like GFP, YFP, RFP etc), and participants were able to create their own art pieces on agar plates. We let the artwork, well plates, grow overnight in the incubator. We took pictures of them under UV light and uploaded them to the Telus Spark Facebook page so that the participants could check out their paintings. Click the link below to see all their amazing work!
Both events were a huge hit as we reached out to over 400 people and loved the experience of talking to the general public about synthetic biology. Furthermore, we introduced our prospective project idea; participants were amazed at the idea of using a living system to detect pathogens in blood. We’re ecstatic to have sparked curiosity in the minds of general public, and shown how exciting science can really be.
