The Sponge Patrol Project

For the third participation to the iGEM competition, we decided to introduce a new chassis to the competition: Pseudovibrio denitrificans.
blabla bla Sum up of overview parts
un cadre ou une liste d'items
Le détail des 3 soius-unités avec une image sympa pour chacun, on clique dessus pour avoir la suite Chassis : draw a bacteria in water the same as the one in the logo

A specific chassis for a specific environment:
Seas & Oceans

As seen in the overview our biosensors should work optimally in native marine conditions, a conditions for which no current chassis in iGEM is ready for.

Moreover it has to attach to sponges and stick with them without disturbing the microbiome for as long as possible.

Therefore came up the necessity to use a bacterium naturally present on the sponges, may it be an epibiont or a symbiont.

Bacterium properties:

• Must be massively present on the sponge surface : avoid being in an unfavourable position for food competition.
• Must be found mainly in sponges : avoid spreading to species in contact with sponges.
• Must be the phenotypically closest possible to a known bacterium : avoid cell cultures difficulties.




Sponge properties:



• Must be numerously present in the ocean : avoiding putting species in danger.
• Must be a natural host of the bacterium : avoid adapting its microbiom to the new epibiont.
• Must be easily culturable in laboratoty : avoid sponge culture difficulties.


The closest combination sponge/bacterium that could fit the requirement is Spongia Officinalis / Pseudovibrio denitrificans
Hence, Pseudovibrio denitrificans living in symbiosis with the sponge : Spongia Officinalis.

Pseudovibrio denitrificans characteristics



Transposons: a transposons
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Compounds/target : some usine in the background I think the title need to be change and as to be rename target
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