Team:BostonU/Workflow

From 2014.igem.org

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• Add parts to <a href="https://2014.igem.org/Team:BostonU/MoClo">MoClo library</a>. These parts were found to be necessary for our priority encoder:<br><br>
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<p>• Add parts to <a href="https://2014.igem.org/Team:BostonU/MoClo">MoClo library</a>. These parts were found to be necessary for our priority encoder:<br><br>
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&nbsp;&nbsp;&nbsp;&nbsp;• 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different <a href="https://2014.igem.org/Team:BostonU/Backbones">origin of replication</a>:<br>
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• 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different <a href="https://2014.igem.org/Team:BostonU/Backbones">origin of replication</a>:<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;• ColE1<br>
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• ColE1<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;• p15A<br>
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• p15A<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;• pSC101<br><br>
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• pSC101<br><br>
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&nbsp;&nbsp;&nbsp;&nbsp;• X <a href="https://2014.igem.org/Team:BostonU/FusionProteins">fusion proteins</a>:<br>
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• X <a href="https://2014.igem.org/Team:BostonU/FusionProteins">fusion proteins</a>:<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;• TetR_GFP<br>
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• TetR_GFP<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;• ...<br><br>
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• ...<br><br>
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&nbsp;&nbsp;&nbsp;&nbsp;• X <a href="https://2014.igem.org/Team:BostonU/ProjectTandemPromoters">tandem promoters</a>:<br>
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• X <a href="https://2014.igem.org/Team:BostonU/ProjectTandemPromoters">tandem promoters</a>:<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;• pTet_pLac<br>
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• pTet_pLac<br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;• ...<br><br>
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• ...<br><br>
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</p>

Revision as of 17:31, 23 September 2014



Workflow

Phase I - Build and test basic parts.

General Chimera Workflow

Case Study: BU Priority Encoder

general I

• Add parts to MoClo library. These parts were found to be necessary for our priority encoder:

• 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different origin of replication:
• ColE1
• p15A
• pSC101

• X fusion proteins:
• TetR_GFP
• ...

• X tandem promoters:
• pTet_pLac
• ...








Our Sponsors

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