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Team:BostonU/Workflow
From 2014.igem.org
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| - | • Add parts to <a href="https://2014.igem.org/Team:BostonU/MoClo">MoClo library</a>. These parts were found to be necessary for our priority encoder:<br> | + | • Add parts to <a href="https://2014.igem.org/Team:BostonU/MoClo">MoClo library</a>. These parts were found to be necessary for our priority encoder:<br><br> |
• 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different <a href="https://2014.igem.org/Team:BostonU/Backbones">origin of replication</a>:<br> | • 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different <a href="https://2014.igem.org/Team:BostonU/Backbones">origin of replication</a>:<br> | ||
• ColE1<br> | • ColE1<br> | ||
• p15A<br> | • p15A<br> | ||
| - | • pSC101<br> | + | • pSC101<br><br> |
• X <a href="https://2014.igem.org/Team:BostonU/FusionProteins">fusion proteins</a>:<br> | • X <a href="https://2014.igem.org/Team:BostonU/FusionProteins">fusion proteins</a>:<br> | ||
• TetR_GFP<br> | • TetR_GFP<br> | ||
| - | • ...<br> | + | • ...<br><br> |
• X <a href="https://2014.igem.org/Team:BostonU/ProjectTandemPromoters">tandem promoters</a>:<br> | • X <a href="https://2014.igem.org/Team:BostonU/ProjectTandemPromoters">tandem promoters</a>:<br> | ||
• pTet_pLac<br> | • pTet_pLac<br> | ||
| - | • ...<br> | + | • ...<br><br> |
Revision as of 17:28, 23 September 2014
Phase I - Build and test basic parts. | |
|---|---|
General Chimera Workflow |
Case Study: BU Priority Encoder |
| general I |
• Add parts to MoClo library. These parts were found to be necessary for our priority encoder: • 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different origin of replication: • ColE1 • p15A • pSC101 • X fusion proteins: • TetR_GFP • ... • X tandem promoters: • pTet_pLac • ... |
