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Team:BostonU/Workflow
From 2014.igem.org
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• Add parts to <a href="https://2014.igem.org/Team:BostonU/MoClo>MoClo library</a>. These parts were found to be necessary for our priority encoder: | • Add parts to <a href="https://2014.igem.org/Team:BostonU/MoClo>MoClo library</a>. These parts were found to be necessary for our priority encoder: | ||
| - | • 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different <a href="https://2014.igem.org/Team:BostonU/Backbones">origin of replication</a>: | + | • 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different <a href="https://2014.igem.org/Team:BostonU/Backbones">origin of replication</a>:<br> |
| - | • ColE1 | + | • ColE1<br> |
| - | • p15A | + | • p15A<br> |
| - | • pSC101 | + | • pSC101<br> |
| - | • X <a href="https://2014.igem.org/Team:BostonU/FusionProteins">fusion proteins</a>: | + | • X <a href="https://2014.igem.org/Team:BostonU/FusionProteins">fusion proteins</a>:<br> |
| - | • TetR_GFP | + | • TetR_GFP<br> |
| - | • ... | + | • ...<br> |
| - | • X <a href="https://2014.igem.org/Team:BostonU/ProjectTandemPromoters">tandem promoters</a>: | + | • X <a href="https://2014.igem.org/Team:BostonU/ProjectTandemPromoters">tandem promoters</a>:<br> |
| - | • pTet_pLac | + | • pTet_pLac<br> |
| - | • ... | + | • ...<br> |
Revision as of 17:27, 23 September 2014
Phase I - Build and test basic parts. | |
|---|---|
General Chimera Workflow |
Case Study: BU Priority Encoder |
| general I |
• Add parts to origin of replication: • ColE1 • p15A • pSC101 • X fusion proteins: • TetR_GFP • ... • X tandem promoters: • pTet_pLac • ... |
