Team:BostonU/Workflow

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         <th scope="col" class="tableborderleft" style="padding-left: 15px"><center><h3>Case Study: BU Priority Encoder</h3></center>
         <th scope="col" class="tableborderleft" style="padding-left: 15px"><center><h3>Case Study: BU Priority Encoder</h3></center>
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         <th scope="col">This notebook details the process undertaken to replace the high copy pMB1 origin in our existing MoClo Level 1 and Level 2 destination vectors (named DVL1 and DVL2, respectively) with lower copy origins. Namely, the ColE1 (~50 plasmids/cell), p15A (~10 plasmids/cell), and pSC101 (~5 plasmids/cell) origins were selected to replace the high copy origin in DVL1 and DVL2.<br><br>All protocols used in this notebook are found in our <a href="https://2014.igem.org/Team:BostonU/Protocols">protocols</a> section.</th>
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         <th scope="col">general I</th>
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• Add parts to <a href="https://2014.igem.org/Team:BostonU/MoClo>MoClo library</a>. These parts were found to be necessary for our priority encoder:
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        • 3 MoClo level 1 and 3 MoClo level 2 backbones, each with a different <a href="https://2014.igem.org/Team:BostonU/Backbones">origin of replication</a>:
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                • ColE1
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                • p15A
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                • pSC101
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        • X <a href="https://2014.igem.org/Team:BostonU/FusionProteins">fusion proteins</a>:
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                • TetR_GFP
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                • ...
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        • X <a href="https://2014.igem.org/Team:BostonU/ProjectTandemPromoters">tandem promoters</a>:
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                • pTet_pLac
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                • ...
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<th scope="col" class="tableborderleft" style="padding-left: 15px">blehbleh</th>
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Revision as of 17:27, 23 September 2014



Workflow

Phase I - Build and test basic parts.

General Chimera Workflow

Case Study: BU Priority Encoder

general I • Add parts to origin of replication: • ColE1 • p15A • pSC101 • X fusion proteins: • TetR_GFP • ... • X tandem promoters: • pTet_pLac • ...







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