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Team:BostonU/Workflow
From 2014.igem.org
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| - | <th scope="col" colspan="2"><h2>Phase I - Build and test basic parts.</h2 | + | <th scope="col" colspan="2"><h2>Phase I - Build and test basic parts.</h2> |
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| - | <th scope="col"><center><h3>General Chimera Workflow</center></h3></th> | + | <th scope="col" width="50%"><center><h3>General Chimera Workflow</center></h3></th> |
| - | <th scope="col"><center><h3>Case Study: BU Priority Encoder</h3></center> | + | <th scope="col"><center><h3>Case Study: BU Priority Encoder</h3></center> |
</tr> | </tr> | ||
Revision as of 17:08, 23 September 2014
Phase I - Build and test basic parts. | |
|---|---|
General Chimera Workflow |
Case Study: BU Priority Encoder |
| This notebook details the process undertaken to replace the high copy pMB1 origin in our existing MoClo Level 1 and Level 2 destination vectors (named DVL1 and DVL2, respectively) with lower copy origins. Namely, the ColE1 (~50 plasmids/cell), p15A (~10 plasmids/cell), and pSC101 (~5 plasmids/cell) origins were selected to replace the high copy origin in DVL1 and DVL2. All protocols used in this notebook are found in our protocols section. |
blehbleh |
