"
Team:BostonU/Workflow
From 2014.igem.org
(Difference between revisions)
| Line 12: | Line 12: | ||
<table width="100%" border="0" cellspacing="15" cellpadding="0"> | <table width="100%" border="0" cellspacing="15" cellpadding="0"> | ||
| - | </tr> | + | |
| + | <tr> | ||
| + | <th scope="col" colspan="2"><h2>Phase I - Build and test basic parts.</h2><br> | ||
| + | </th></tr> | ||
<tr> | <tr> | ||
<th scope="col"><center><h3>General Chimera Workflow</center></h3></th> | <th scope="col"><center><h3>General Chimera Workflow</center></h3></th> | ||
| Line 19: | Line 22: | ||
</tr> | </tr> | ||
| - | + | ||
| - | + | ||
| - | + | ||
<tr> | <tr> | ||
<th scope="col">This notebook details the process undertaken to replace the high copy pMB1 origin in our existing MoClo Level 1 and Level 2 destination vectors (named DVL1 and DVL2, respectively) with lower copy origins. Namely, the ColE1 (~50 plasmids/cell), p15A (~10 plasmids/cell), and pSC101 (~5 plasmids/cell) origins were selected to replace the high copy origin in DVL1 and DVL2.<br><br>All protocols used in this notebook are found in our <a href="https://2014.igem.org/Team:BostonU/Protocols">protocols</a> section.</th> | <th scope="col">This notebook details the process undertaken to replace the high copy pMB1 origin in our existing MoClo Level 1 and Level 2 destination vectors (named DVL1 and DVL2, respectively) with lower copy origins. Namely, the ColE1 (~50 plasmids/cell), p15A (~10 plasmids/cell), and pSC101 (~5 plasmids/cell) origins were selected to replace the high copy origin in DVL1 and DVL2.<br><br>All protocols used in this notebook are found in our <a href="https://2014.igem.org/Team:BostonU/Protocols">protocols</a> section.</th> | ||
Revision as of 17:07, 23 September 2014
Phase I - Build and test basic parts. | |
|---|---|
General Chimera Workflow |
Case Study: BU Priority Encoder |
| This notebook details the process undertaken to replace the high copy pMB1 origin in our existing MoClo Level 1 and Level 2 destination vectors (named DVL1 and DVL2, respectively) with lower copy origins. Namely, the ColE1 (~50 plasmids/cell), p15A (~10 plasmids/cell), and pSC101 (~5 plasmids/cell) origins were selected to replace the high copy origin in DVL1 and DVL2. All protocols used in this notebook are found in our protocols section. |
blehbleh |
