Team:Calgary/Notebook/ProtocolManual/General

From 2014.igem.org

(Difference between revisions)
(Created page with "{{Team:Calgary/ContentPage}} <html> <section class="Content Text Color-Dark"> <h1>General Protocols</h1> <p>Enter Text Here</p> </section> </html>")
Line 4: Line 4:
<section class="Content Text Color-Dark">
<section class="Content Text Color-Dark">
<h1>General Protocols</h1>
<h1>General Protocols</h1>
-
<p>Enter Text Here</p>
+
<p>
 +
<b>Agarose Gel Electrophoresis</b>
 +
<ol type=1>
 +
<li>Add TAE Buffer (100 mL) and Agarose (1g) to a flask [for a 1% gel]</li>
 +
<li>Cover with plastic wrap, poke hole in top. Then microwave flask until agarose is fully dissolved; avoid boiling</li>
 +
<li>Take flask to fume hood, and allow to cool to touch. Add SYBR safe (4 μL) to agarose, gently swirl to mix</li>
 +
<li>Gently pour agarose into assembled gel casting tray, removing any bubbles with a pipette tip</li>
 +
<li>Allow gel to solidify until translucent. Then transfer to running apparatus filled with TAE buffer</li>
 +
<li>Load samples containing 3 μL loading dye and ~10-15 μL of DNA</li>
 +
<li>Run gel at 110V until the dye is ~2/3 of the way down the gel (approx. 40 mins)</li>
 +
 
 +
</p>
</section>
</section>
</html>
</html>

Revision as of 18:34, 10 October 2014

General Protocols

Agarose Gel Electrophoresis

  1. Add TAE Buffer (100 mL) and Agarose (1g) to a flask [for a 1% gel]
  2. Cover with plastic wrap, poke hole in top. Then microwave flask until agarose is fully dissolved; avoid boiling
  3. Take flask to fume hood, and allow to cool to touch. Add SYBR safe (4 μL) to agarose, gently swirl to mix
  4. Gently pour agarose into assembled gel casting tray, removing any bubbles with a pipette tip
  5. Allow gel to solidify until translucent. Then transfer to running apparatus filled with TAE buffer
  6. Load samples containing 3 μL loading dye and ~10-15 μL of DNA
  7. Run gel at 110V until the dye is ~2/3 of the way down the gel (approx. 40 mins)

Retrieved from "http://2014.igem.org/Team:Calgary/Notebook/ProtocolManual/General"