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Team:Paris Saclay/Project
From 2014.igem.org
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<li>A - The chassis coli Odor free</li> | <li>A - The chassis coli Odor free</li> | ||
| + | <p> | ||
Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene. | Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene. | ||
| - | In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but it wasn't the right strain. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all. | + | In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but it wasn't the right strain. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all. |
| + | <p> | ||
| + | <a href="https://static.igem.org/mediawiki/2014/f/fc/OdorFree.jpg"><img src="img/montagne_mini.jpg" alt="Preparation of the coli odor free chassis" /></a> | ||
| + | </p> | ||
<li>B - Construction of the fusion protein (color)</li> | <li>B - Construction of the fusion protein (color)</li> | ||
<li>C - Salicylate Inducible Suppressing System</li> | <li>C - Salicylate Inducible Suppressing System</li> | ||
Revision as of 14:48, 4 August 2014
| Page under construction. Our team is working hard and excited to develop a very good Wiki. | ||
The different parts of our project: |
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Escherichia coli stinks because of the tnaA gene which produces an enzyme that transforms the L-tryptophan into indole, responsible for the stench. If we want our lemon to smell like one, we have to delete this gene. In the lab, we already had a strain in which the tnaA was replaced by a kanamycin resistance, but it wasn't the right strain. So we switched the tnaA sequence with the kanamycin resistance in our bacterium by phage transduction. After the recombination, we used a flipase to delete the kanamycin resistance. The remaining bacterium doesn't smell at all.
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