"

From 2014.igem.org

Revision as of 00:53, 18 October 2014 (view source) Tolland (Talk | contribs) m (→Methods) ← Older edit		Revision as of 00:53, 18 October 2014 (view source) Tolland (Talk | contribs) (→Methods) Newer edit →
Line 44:		Line 44:
	File:14939024114 e7aa0d51ca b.jpg		File:14939024114 e7aa0d51ca b.jpg
	File:15536077496 9326a7fb0d b.jpg		File:15536077496 9326a7fb0d b.jpg
-	https://2014.igem.org/File:15557111841_44a7168e0f_b.jpg	+	File:15557111841_44a7168e0f_b.jpg
	</gallery>		</gallery>

---

Revision as of 00:53, 18 October 2014

Home	Project	Team	Policy & Practice	Protocols	Safety

Contents 1 Intro 2 Aim 3 Methods 4 Results

Intro

The basic proposal for juicyprint is the growth of cellulose by bacteria under the control of light. The 660nm light that the Cph1 protein is sensitive to inhibits the growth. Therefore we designed a system to generate the production of a pellicle sheet in the shape of a projected template of the negative image.

So a light pattern like this	should result in a pellicle growth pattern like this

Aim

The primary aim is to demonstrate a mechanical system which can be used to provide a light source and as a basic proof of concept.

This is a gallery of a series of images from basic prototypes and some initial tests

Methods

We were unable to fully test the system with the target system. Therefore we simulated the process with just a liquid target.

660nm light is a bright red colour;

Image courtesy of Beer Color Laboratories; http://www.beercolor.com/color_basics1.htm

We tested a number of light sources to be able to generate 660nm patterns easily.

Results

A thick 660nm camera filter gets very hot when placed near the projector for a period of time.

Therefore I settled on using a home-made red permanent marker pen to make a 35mm slide suitable for projection. The slide was much thinner and didn't absorb so much light and get hot.

London Biohackers