Team:Concordia/Notebook
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<span id="note">Note: Coverslips for counting chambers are specially made and are thicker than those for conventional microscopy, since they must be heavy enough to overcome the surface tension of a drop of liquid.</span></p></li> | <span id="note">Note: Coverslips for counting chambers are specially made and are thicker than those for conventional microscopy, since they must be heavy enough to overcome the surface tension of a drop of liquid.</span></p></li> | ||
<li><p>Place the coverslip over the counting surface prior to putting on the cell suspension.</p></li> | <li><p>Place the coverslip over the counting surface prior to putting on the cell suspension.</p></li> | ||
- | <li><p>Introduce the suspension into one of the V-shaped wells with a pasteur or other type of pipet. | + | <li><p>Introduce the suspension into one of the V-shaped wells with a pasteur or other type of pipet.<br /> |
<span id="note">Note: The area under the coverslip fills by capillary action. Enough liquid should be introduced so that the mirrored surface is just covered. </span></p></li> | <span id="note">Note: The area under the coverslip fills by capillary action. Enough liquid should be introduced so that the mirrored surface is just covered. </span></p></li> | ||
<li><p>Place the counting chamber on the microscope stage and bring the counting grid is into focus at low power.</p></li> | <li><p>Place the counting chamber on the microscope stage and bring the counting grid is into focus at low power.</p></li> |
Revision as of 13:58, 17 October 2014
Growth Curve and Cell Count
Cell count via Hemocytonomer
Clean the cover glass mounting support and the coverslip of the hemocytometer with a lens paper and some ethanol.
Note: Coverslips for counting chambers are specially made and are thicker than those for conventional microscopy, since they must be heavy enough to overcome the surface tension of a drop of liquid.Place the coverslip over the counting surface prior to putting on the cell suspension.
Introduce the suspension into one of the V-shaped wells with a pasteur or other type of pipet.
Note: The area under the coverslip fills by capillary action. Enough liquid should be introduced so that the mirrored surface is just covered.Place the counting chamber on the microscope stage and bring the counting grid is into focus at low power.
Antibiotic Spot Test
Gibson Assembly
Transformation and Genomic DNA Extraction
Cryofreeze
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