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Team:Evry/Overview/Project
From 2014.igem.org
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| - | For | + | For our third participation to the iGEM competition, we decided to use <b><FONT COLOR=#666699>a new chassis </FONT></b>to the competition, which is able to work in depth of oceans through a forceful duo: <b>sponge and bacterium</b>. |
| - | Sponge can filtrate up to | + | Sponge can filtrate up to 20m<sup>3</sup> of water/day/kg making it one of the most powerful filtrating system alive. |
We focused on the sponge <b><FONT COLOR=#FFCC33>Spongia Officinalis</FONT></b>, which lives in symbiosis with the bacterium: <i><FONT COLOR=#006633>Pseudovibrio denitrificans</font></i>. | We focused on the sponge <b><FONT COLOR=#FFCC33>Spongia Officinalis</FONT></b>, which lives in symbiosis with the bacterium: <i><FONT COLOR=#006633>Pseudovibrio denitrificans</font></i>. | ||
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Revision as of 14:35, 16 October 2014
Overview - Project
Water is the most precious and fragile resource on Earth. However seas and oceans are polluted by a wide range of compounds stemming from human activities.
Faced with this issue, our team wants to heighten awareness about environmental pollution and particularly its devastating consequences on the aquatic life.
In this line of thinking we are driven to design a bioremediation tool based on the considerable filtration capacity of sponges to decrease the marine pollution and protect aquatic ecosystems.
Our project is based on the engineering of this bacterium in the microbiome in order to develop a filtrating system allowing to sense and even degrade pollutants in water
The Sponge Patrol Project
Chassis
Transposons
Target
Chassis
For our third participation to the iGEM competition, we decided to use a new chassis to the competition, which is able to work in depth of oceans through a forceful duo: sponge and bacterium.
Sponge can filtrate up to 20m3 of water/day/kg making it one of the most powerful filtrating system alive.
We focused on the sponge Spongia Officinalis, which lives in symbiosis with the bacterium: Pseudovibrio denitrificans.
Pseudovibrio denitrificans is a marine bacterium not yet characterize and has never been used in iGEM.
Likewise, we add Spongia Officinalis, a sponge with impressive features and capacities.
This is why we dedicate a part introducing them.
Transposons
Our synthetic system is Pseudovibrio denitrificans, however its origin from depth oceans make hard its transformation.
In order to be able to transform our bacterium and insert genes of interest, we have introduce a new format : the transposon.
This tool allows to insert genes easily into unknown bacteria.
Indeed, our construct is compound of a universal ORI.
Target
This year, our team objective is to develop a filtrating system which could allow to sense and could even degrade pollutants in the future.
For this project, we have chosen to work on elements most great classes of marine pollutants belonging to most important classes of pollutants of the marine environment.
Hence, we have chosen to focus on phenols, PCBs, nitrite and heavy metals.
