Team:UFAM Brazil/Methods

From 2014.igem.org

(Difference between revisions)
Line 6: Line 6:
<head>
<head>
<style>
<style>
-
p a{color:#32CD32; font-size:20px;font-weight:bold;}
+
p a{color:#508c25; font-size:20px;font-weight:bold;}
 +
p a:hover, p a:visited{#32CD32 font-size:20px;}
</style>
</style>

Revision as of 15:17, 16 October 2014

Methods

Cell lines, DNA synthesis and Plasmids

Cryopreservation of bacteria

Chemically competent cells preparation

Preparation of plasmid DNA on a small scale

Rapid transformation using Sorbitol

Heat-Shock Transformation

BioBrick Restriction Digest

Biobrick ligation

LB culture medium + Mercury chloride (ML)

Cell growth quantification of mercury resistant Escherichia coli DH5α

Quantification of Mercury bioaccumulated by metal binding protein (MBP)

Quantification of Green Fluorescent Protein

Retrieved from "http://2014.igem.org/Team:UFAM_Brazil/Methods"