Team:BostonU/Training

(Difference between revisions)

Revision as of 20:05, 21 June 2014

Training Notebook

This notebook details the concepts, methods, and protocols learned throughout the iGEM process by all of the BU team members.
May
Week of May 12
• Learned about synthetic biology, iGEM, and MoClo. • Became familiar with lab part and BioBrick part nomenclature. • Learned how to use miniEugene from CIDAR lab members. • Developed plan for next two weeks that would guide through training of essential lab protocols - specifically plate streaking, minipreps, MoClo, sequencing, and colony PCR. • Began using miniEugene to design architecture of constructs. • Struck out plates with 15 L0 parts, 6 L1 parts, and 2 L2 parts and grew colonies in liquid cultures overnight.

Our Sponsors

@@ Line 15: / Line 15: @@
      <table width="100%" border="0" cellspacing="0" cellpadding="0">
        <tr>
-         <th colspan="2" scope="col"><header3>This notebook details the concepts, methods, and protocols learned throughout the iGEM process by all of the BU team members.</header3> </th>
+         <th colspan="2" scope="col">This notebook details the concepts, methods, and protocols learned throughout the iGEM process by all of the BU team members.</th>
        </tr>
        <tr>
@@ Line 22: / Line 22: @@
 <tr>
          <th colspan="2" scope="col"><h3>Week of May 12</h3></th>
+      </tr>
+<tr>
+        <th colspan="2" scope="col">•	Learned about synthetic biology, iGEM, and MoClo. <br>
+•	Became familiar with lab part and BioBrick part nomenclature.<br>
+•	Learned how to use miniEugene from CIDAR lab members.<br>
+•	Developed plan for next two weeks that would guide through training of essential lab protocols - specifically plate streaking, minipreps, MoClo, sequencing, and colony PCR.<br>
+•	Began using miniEugene to design architecture of constructs.<br>
+•	Struck out plates with 15 L0 parts, 6 L1 parts, and 2 L2 parts and grew colonies in liquid cultures overnight.<br>
+</th>
        </tr>
      </table>