Team:KIT-Kyoto/Notebook/Labnote

From 2014.igem.org

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</table>
</table>
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<!--Miniprep終わり-->
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<br>
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<strong>Restriction Digest</strong>
<strong>Restriction Digest</strong>
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<br>
<br>
 +
<!--Restriction Digest-->
 +
<strong>Restriction Digest</strong>
 +
<table class="materials">
 +
<tr>
 +
<th rowspan="2">Lane</th>
 +
<th colspan="2">Sample/Marker</th>
 +
<th rowspan="2">Insert</th>
 +
<th rowspan="2">Enzyme</th>
 +
<th rowspan="2">Buffer</th>
 +
</tr>
 +
<tr>
 +
<th>Host</th>
 +
<th>Vector</th>
 +
</tr>
 +
<tr>
 +
<td>1</td>
 +
<td colspan="5">1kb Ladder</td>
 +
<tr>
 +
<td>2</td>
 +
<td colspan="5">λDNA-HindⅢ</td>
 +
<tr>
 +
<td>3</td>
 +
<td>1-a</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>4</td>
 +
<td>1-b</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>5</td>
 +
<td>2-a</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2/td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>6</td>
 +
<td>2-b</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>7</td>
 +
<td>3-a</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>8</td>
 +
<td>3-b</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<td>9</td>
 +
<td>6-a</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS62</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
<tr>
 +
<td>10</td>
 +
<td>6-b</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS62</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>11</td>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>12</td>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2/td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>13</td>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>14</td>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS62</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
</table>
 +
<!--Ristriction Digest-->
 +
<br>
 +
Applied λDNA-HindⅢ to compare and contrast AGE of July 14th and June 11th.
</p>
</p>
<!--本文終わり-->
<!--本文終わり-->

Revision as of 07:53, 2 October 2014

LabNote

June

June 10, 2014

OKAMOTO
Miniprep

Host Sample
Vector Insert
BL21(DE3) pGEX6P-2 CuMTSE1
BL21(DE3) pGEX6P-2 CuMTSE2
BL21(DE3) pGEX6P-2 CuMTS3
BL21(DE3) pGEX6P-2 CuMTS62

Restriction Digest
Host Sample Enzyme Buffer
Vector Insert
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 Sal1, Not1 Buffer H

June 11, 2014

KOBAYASHI
AGE

Lane Sample/Marker Enzyme
Vector Insert
1 λDNA-HindⅢ
2 pGEX6P-2 CuMTSE1 N.D
3 pGEX6P-2 CuMTSE1 EcoR1
4 pGEX6P-2 CuMTSE1 Sal1,Not1
5 pGEX6P-2 CuMTSE2 N.D
6 pGEX6P-2 CuMTSE2 EcoR1
7 pGEX6P-2 CuMTSE2 Sal1, Not1
8 pGEX6P-2 CuMTS3 N.D
9 pGEX6P-2 CuMTS3 EcoR1
10 pGEX6P-2 CuMTS62 N.D
11 pGEX6P-2 CuMTS62 EcoR1
12 pGEX6P-2 CuMTS62 Sal1,Not1

June 12, 2014

KOBAYASHI
Transformation

Host Sample
Vector Insert
BL21(DE3)pLysS pGEX6P-2 CuMTSE1
BL21(DE3)pLysS pGEX6P-2 CuMTSE2
BL21(DE3)pLysS pGEX6P-2 CuMTS3
BL21(DE3)pLysS pGEX6P-2 CuMTS62

June 13, 2014

KOBAYASHI
Colony Isolation
Select 6 colonies per plate from 4 plates from June 12th.

Host Sample Section
Vector Insert
BL21(DE3)pLysS pGEX6P-2 CuMTSE1 1-a,1-b
BL21(DE3)pLysS pGEX6P-2 CuMTSE2 2-a,2-b
BL21(DE3)pLysS pGEX6P-2 CuMTS3 3-a,3-b
BL21(DE3)pLysS pGEX6P-2 CuMTSE62 6-a,6-b

June 14, 2014

ONISHI
Miniprep

Host Sample
Vector Insert
1-a pGEX6P-2 CuMTSE1
1-b pGEX6P-2 CuMTSE1
2-a pGEX6P-2 CuMTSE2
2-b pGEX6P-2 CuMTSE2
3-a pGEX6P-2 CuMTS3
3-b pGEX6P-2 CuMTS3
6-a pGEX6P-2 CuMTS62
6-b pGEX6P-2 CuMTS62

Miniprep
Host Sample
Vector Insert
BL21(DE3) pGEX6P-2 CuMTSE1
BL21(DE3) pGEX6P-2 CuMTSE2
BL21(DE3) pGEX6P-2 CuMTS3
BL21(DE3) pGEX6P-2 CuMTS62

Restriction Digest
Host Sample Enzyme Buffer
Vector Insert
1-a pGEX6P-2 CuMTSE1 EcoR1 Buffer H
1-b pGEX6P-2 CuMTSE1 EcoR1 Buffer H
2-a pGEX6P-2 CuMTSE2 EcoR1 Buffer H
2-b pGEX6P-2 CuMTSE2 EcoR1 Buffer H
3-a pGEX6P-2 CuMTS3 EcoR1 Buffer H
3-b pGEX6P-2 CuMTS3 EcoR1 Buffer H
6-a pGEX6P-2 CuMTS62 EcoR1 Buffer H
6-b pGEX6P-2 CuMTS62 EcoR1 Buffer H
BL21(DE3) pGEX6P-2 CuMTSE1 EcoR1 Buffer H
BL21(DE3) pGEX6P-2 CuMTSE2 EcoR1 Buffer H
BL21(DE3) pGEX6P-2 CuMTS3 EcoR1 Buffer H
BL21(DE3) pGEX6P-2 CuMTS62 EcoR1 Buffer H

Restriction Digest
Lane Sample/Marker Insert Enzyme Buffer
Host Vector
1 1kb Ladder
2 λDNA-HindⅢ
3 1-a pGEX6P-2 CuMTSE1 EcoR1 Buffer H
4 1-b pGEX6P-2 CuMTSE1 EcoR1 Buffer H
5 2-a pGEX6P-2 CuMTSE2/td> EcoR1 Buffer H
6 2-b pGEX6P-2 CuMTSE2 EcoR1 Buffer H
7 3-a pGEX6P-2 CuMTS3 EcoR1 Buffer H
8 3-b pGEX6P-2 CuMTS3 EcoR1 Buffer H
9 6-a pGEX6P-2 CuMTS62 EcoR1 Buffer H
10 6-b pGEX6P-2 CuMTS62 EcoR1 Buffer H
11 BL21(DE3) pGEX6P-2 CuMTSE1 EcoR1 Buffer H
12 BL21(DE3) pGEX6P-2 CuMTSE2/td> EcoR1 Buffer H
13 BL21(DE3) pGEX6P-2 CuMTS3 EcoR1 Buffer H
14 BL21(DE3) pGEX6P-2 CuMTS62 EcoR1 Buffer H

Applied λDNA-HindⅢ to compare and contrast AGE of July 14th and June 11th.

July

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