LabNote

June

June 10, 2014

OKAMOTO
Miniprep

Host	Sample
	Vector	Insert
BL21(DE3)	pGEX6P-2	CuMTSE1
BL21(DE3)	pGEX6P-2	CuMTSE2
BL21(DE3)	pGEX6P-2	CuMTS3
BL21(DE3)	pGEX6P-2	CuMTS62

Restriction Digest

Host	Sample		Enzyme	Buffer
	Vector	Insert
BL21(DE3)PlysS	pGEX6P-2	CuMTSE1	EcoR1	Buffer H
BL21(DE3)PlysS	pGEX6P-2	CuMTSE2	EcoR1	Buffer H
BL21(DE3)PlysS	pGEX6P-2	CuMTS3	EcoR1	Buffer H
BL21(DE3)PlysS	pGEX6P-2	CuMTSE62	EcoR1	Buffer H
BL21(DE3)PlysS	pGEX6P-2	CuMTSE1	Sal1, Not1	Buffer H
BL21(DE3)PlysS	pGEX6P-2	CuMTSE2	Sal1, Not1	Buffer H
BL21(DE3)PlysS	pGEX6P-2	CuMTS3	Sal1, Not1	Buffer H
BL21(DE3)PlysS	pGEX6P-2	CuMTSE62	Sal1, Not1	Buffer H

June 11, 2014

KOBAYASHI
AGE

Lane	Sample/Marker		Enzyme
	Vector	Insert
1	λDNA-HindⅢ
2	pGEX6P-2	CuMTSE1	N.D
3	pGEX6P-2	CuMTSE1	EcoR1
4	pGEX6P-2	CuMTSE1	Sal1,Not1
5	pGEX6P-2	CuMTSE2	N.D
6	pGEX6P-2	CuMTSE2	EcoR1
7	pGEX6P-2	CuMTSE2	Sal1, Not1
8	pGEX6P-2	CuMTS3	N.D
9	pGEX6P-2	CuMTS3	EcoR1
10	pGEX6P-2	CuMTS62	N.D
11	pGEX6P-2	CuMTS62	EcoR1
12	pGEX6P-2	CuMTS62	Sal1,Not1

June 12, 2014

KOBAYASHI
Transformation

Host	Sample
	Vector	Insert
BL21(DE3)pLysS	pGEX6P-2	CuMTSE1
BL21(DE3)pLysS	pGEX6P-2	CuMTSE2
BL21(DE3)pLysS	pGEX6P-2	CuMTS3
BL21(DE3)pLysS	pGEX6P-2	CuMTS62

June 13, 2014

KOBAYASHI
Colony Isolation
Select 6 colonies per plate from 4 plates from June 12th.

Host	Sample		Section
	Vector	Insert
BL21(DE3)pLysS	pGEX6P-2	CuMTSE1	1-a,1-b
BL21(DE3)pLysS	pGEX6P-2	CuMTSE2	2-a,2-b
BL21(DE3)pLysS	pGEX6P-2	CuMTS3	3-a,3-b
BL21(DE3)pLysS	pGEX6P-2	CuMTSE62	6-a,6-b

July

7/25

a
a
a
a
a




a
a
a
a
a




a
a
a
a
a

adfsfhao fhkna iehfalo difnzkl ncziehz odvnlkz dvaaa aaaaaaa aaa aaaaaa aaaaa aaaaakkkk kksks kksksk ksksk ksk ksks ksk ksksks kksksk skskksk s ksk skskksksksks kksksk ksk skks kks kskksksk

August

a
a
a
a
a



a
a
a
a
a




a
a
a
a
a




a
a
a
a
a




a
a
a
a
a

September

a
a
a
a
a



a
a
a
a
a




a
a
a
a
a




a
a
a
a
a




a
a
a
a
a

October

a
a
a
a
a



a
a
a
a
a




a
a
a
a
a




a
a
a
a
a




a
a
a
a
a