Team:KIT-Kyoto/Notebook/Labnote
From 2014.igem.org
(Difference between revisions)
Line 303: | Line 303: | ||
</table> | </table> | ||
<!--Transformation終わり--> | <!--Transformation終わり--> | ||
+ | |||
+ | <!-- タイトル --> | ||
+ | <h3>June 13, 2014</h3> | ||
+ | <!-- タイトル終わり --> | ||
+ | <!-- 本文開始 --> | ||
+ | <p class="sentence"> | ||
+ | <!-- 実験者名 --> | ||
+ | KOBAYASHI<br> | ||
+ | <!-- 実験者名終わり --> | ||
+ | <!--Colony Isolation--> | ||
+ | <strong>Colony Isolation</strong><br> | ||
+ | Select 6 colonies per plate from 4 plates from June 12th.<br> | ||
+ | <table class="materials"> | ||
+ | <tr> | ||
+ | <th rowspan="2">Host</th> | ||
+ | <th colspan="2">Sample</th> | ||
+ | <th rowspan="2">Section</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Vector</th> | ||
+ | <th>Insert</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BL21(DE3)pLysS</td> | ||
+ | <td>pGEX6P-2</td> | ||
+ | <td>CuMTSE1</td> | ||
+ | <td>1-a,1-b</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BL21(DE3)pLysS</td> | ||
+ | <td>pGEX6P-2</td> | ||
+ | <td>CuMTSE2</td> | ||
+ | <td>2-a,2-b</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BL21(DE3)pLysS</td> | ||
+ | <td>pGEX6P-2</td> | ||
+ | <td>CuMTS3</td> | ||
+ | <td>3-a,3-b</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BL21(DE3)pLysS</td> | ||
+ | <td>pGEX6P-2</td> | ||
+ | <td>CuMTSE62</td> | ||
+ | <td>6-a,6-b</td> | ||
+ | </tr> | ||
+ | |||
+ | </table> | ||
+ | <!--Colony Isolation--> | ||
+ | |||
+ | <br> | ||
+ | </p> | ||
+ | <!--本文終わり--> | ||
Revision as of 07:08, 2 October 2014
LabNote
June
June 10, 2014
OKAMOTO
Miniprep
Host | Sample | |
---|---|---|
Vector | Insert | |
BL21(DE3) | pGEX6P-2 | CuMTSE1 |
BL21(DE3) | pGEX6P-2 | CuMTSE2 |
BL21(DE3) | pGEX6P-2 | CuMTS3 |
BL21(DE3) | pGEX6P-2 | CuMTS62 |
Restriction Digest
Host | Sample | Enzyme | Buffer | |
---|---|---|---|---|
Vector | Insert | |||
BL21(DE3)PlysS | pGEX6P-2 | CuMTSE1 | EcoR1 | Buffer H |
BL21(DE3)PlysS | pGEX6P-2 | CuMTSE2 | EcoR1 | Buffer H |
BL21(DE3)PlysS | pGEX6P-2 | CuMTS3 | EcoR1 | Buffer H |
BL21(DE3)PlysS | pGEX6P-2 | CuMTSE62 | EcoR1 | Buffer H |
BL21(DE3)PlysS | pGEX6P-2 | CuMTSE1 | Sal1, Not1 | Buffer H |
BL21(DE3)PlysS | pGEX6P-2 | CuMTSE2 | Sal1, Not1 | Buffer H |
BL21(DE3)PlysS | pGEX6P-2 | CuMTS3 | Sal1, Not1 | Buffer H |
BL21(DE3)PlysS | pGEX6P-2 | CuMTSE62 | Sal1, Not1 | Buffer H |
June 11, 2014
KOBAYASHI
AGE
Lane | Sample/Marker | Enzyme | |
---|---|---|---|
Vector | Insert | ||
1 | λDNA-HindⅢ | ||
2 | pGEX6P-2 | CuMTSE1 | N.D |
3 | pGEX6P-2 | CuMTSE1 | EcoR1 |
4 | pGEX6P-2 | CuMTSE1 | Sal1,Not1 |
5 | pGEX6P-2 | CuMTSE2 | N.D |
6 | pGEX6P-2 | CuMTSE2 | EcoR1 |
7 | pGEX6P-2 | CuMTSE2 | Sal1, Not1 |
8 | pGEX6P-2 | CuMTS3 | N.D |
9 | pGEX6P-2 | CuMTS3 | EcoR1 |
10 | pGEX6P-2 | CuMTS62 | N.D |
11 | pGEX6P-2 | CuMTS62 | EcoR1 |
12 | pGEX6P-2 | CuMTS62 | Sal1,Not1 |
June 12, 2014
KOBAYASHI
Transformation
Host | Sample | |
---|---|---|
Vector | Insert | |
BL21(DE3)pLysS | pGEX6P-2 | CuMTSE1 |
BL21(DE3)pLysS | pGEX6P-2 | CuMTSE2 |
BL21(DE3)pLysS | pGEX6P-2 | CuMTS3 |
BL21(DE3)pLysS | pGEX6P-2 | CuMTS62 |
June 13, 2014
KOBAYASHI
Colony Isolation
Select 6 colonies per plate from 4 plates from June 12th.
Host | Sample | Section | |
---|---|---|---|
Vector | Insert | ||
BL21(DE3)pLysS | pGEX6P-2 | CuMTSE1 | 1-a,1-b |
BL21(DE3)pLysS | pGEX6P-2 | CuMTSE2 | 2-a,2-b |
BL21(DE3)pLysS | pGEX6P-2 | CuMTS3 | 3-a,3-b |
BL21(DE3)pLysS | pGEX6P-2 | CuMTSE62 | 6-a,6-b |
July
7/25
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
adfsfhao fhkna iehfalo difnzkl ncziehz odvnlkz dvaaa aaaaaaa aaa aaaaaa aaaaa aaaaakkkk kksks kksksk ksksk ksk ksks ksk ksksks kksksk skskksk s ksk skskksksksks kksksk ksk skks kks kskksksk
August
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
September
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
October
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a
a