Team:Oxford/how much can we degrade
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+ | <div class="row"> | ||
+ | <a href="#hide2" class="hide" id="hide2"><div class="orange_news_block2"> | ||
+ | <h1black>How much would the pH change by?</h1black> | ||
+ | <img src="https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png" style="float:right;position:relative; width:2%;" /> | ||
+ | </div></a> | ||
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+ | <a href="#show2" class="show" id="show2"><div class="orange_news_block2"> | ||
+ | <h1black>How much would the pH change by?</h1black> | ||
+ | </div></a> | ||
+ | <div class="list"> | ||
+ | <div class="white_news_block"> | ||
+ | <h1blue2>Calculating the pH change</h1blue2> | ||
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+ | The degradation of DCM by DcmA produces hydrochloric acid (HCl) according to the reaction below: | ||
+ |  | ||
+ | There is a resulting pH change as a result of this. Because we are dealing with an organic system which cannot tolerate excessive acidity (pH<6), we must track the anticipated HCl production and resulting pH change. | ||
+ | The following relationships were used: | ||
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+ | These equations were then simulated using a series of linked functions on MatLab and the results are displayed below: | ||
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+ | </div> | ||
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+ | <div class="white_news_block"> | ||
+ | <h1>Summary:</h1> | ||
+ | As you can see from the above graph, the native bacteria (Methylobacterium extorquens DM4) will not be able to degrade a large volume of DCM. This bacteria will not be suitable for our purposes as the solution to chlorinated waste disposal. There are several reasons for this, these include: | ||
+ | <br><br> | ||
+ | <li>The degradation of DCM is a stress response for the DM4 bacteria.</li> | ||
+ | <li>The DM4 bacteria grow very slowly (time to grow up a culture is approximately 2 weeks) and are not very robust to external conditions at all.</li> | ||
+ | <li>The DM4 bacteria are very vulnerable to the toxic intermediates of this reaction.</li> | ||
+ | <li>DM4 are not very well known characterised or known about.</li> | ||
+ | <br><br> | ||
+ | However, using synthetic biology, we can dramatically increase the amount of chlorinated solvents that certain bacteria can degrade. This is because: | ||
+ | <br><br> | ||
+ | <li>We will remove the stress response dependence for the degradation of chlorinated solvents. Also, due to this being a reaction driven by the removal of product, we will be able to significantly increase the rate of degradation.</li> | ||
+ | <li>The bacteria that we’re using are E-coli and pseudomonas strains. These bacteria are very fast growing (relatively) and are much more robust to changes in the external conditions.</li> | ||
+ | <li>These strains are less vulnerable to our toxic intermediates.</li> | ||
+ | <br><br> | ||
+ | This model proves the power of computer modelling and shows the importance of using synthetic biology to solve global problems. | ||
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+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
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Revision as of 21:49, 21 September 2014
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