Team:Oxford/why do we need microcompartments
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- | <h1black> | + | <h1black>Increased reaction rate</h1black> |
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<h1>Due to increased concentration of metabolic enzymes</h1> | <h1>Due to increased concentration of metabolic enzymes</h1> | ||
- | + | Proteins can be targeted into microcompartments via an 18-amino acid leader sequence that forms a well-defined helical structure (Lawrence AD, 2014). We added this leader sequence to dcmA in both E. coli and P. putida. The resulting accumulation of dcmA in the microcompartments leads to an increased local concentration of the metabolic enzyme. This allows more rapid degradation of DCM, which can diffuse freely through the plasma membrane and into microcompartments. | |
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<h1>Prevents interference with other cell metabolic activity</h1> | <h1>Prevents interference with other cell metabolic activity</h1> | ||
- | + | Additionally, the products of DCM degradation are only produced within the confines of the microcompartment. This prevents HCl and formaldehyde from interfering with other metabolic processes in the cell. | |
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Revision as of 22:06, 20 September 2014
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