Modelling & Optimization

The modelling component of our project was aimed at optimizing the system and creating a 3D visual to demonstrate the way the device operates. With quantitative modelling, we characterized the reporter using the plate reader. With the plate reader, we measured cell count and absorbance over night for E. coli transformed with RFP (Red Fluorescent Protein), E. coli transformed with lacZ, and B. subtilis transformed with RFP. In order to make our diagnostic test as fast as possible, our other goal with modelling was to quantify the experiments performed by biology students on the team to determine most optimal conditions for B. subtilis transformation and growth. Modelling and simulations also allowed us to determine the best way to put our physical device together. 3D animation made using Autodesk Maya software was used to explain how the device works. Creating 3D animation allows to visualize how different parts of the system work as well as how they are connected together. A visual also makes it easier for people from different backgrounds to understand what is happening in the device. Our modelling team also created a 3D animation of our device. It shows the physical outline of the device as well as an animation of the biological system. The animation was created using Autodesk Maya 2013 software and ePMV plug in. It serves as a visual to everything taking place in our device making it easier to understand even for general public.