Team:Waterloo

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<title>Waterloo iGem 2014 Team</title>
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<h1>Staphylocide</h1>
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<h3 style="text-align: center">Delivery of an Antibiotic Resistance Gene Silencing Mechanism to a MRSA Population using Bacterial Conjugation</h3>
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  <h2 class="centerUpper blueOnGrey">Problem</h2>
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  <p>MRSA is sweeping the nation, an antibiotic-resistant scourge migrating from hospitals into communities. We need a hero to fight it and Waterloo iGEM has designed a synthetic bacterium that just might do the trick...</p>
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  <h2 class="centerUpper blueOnGrey">Solution</h2>
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  <img class="smallIcon floatRight" src="https://static.igem.org/mediawiki/2014/9/94/Waterloo_staphylocide_face.png" />
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  <p>...Staphylocide! Staphylocide delivers antibiotic resistance gene silencing <em>in vivo</em> and could one day be incorporated into a treatment that would help doctors defeat MRSA once and for all.</p>
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<p>&nbsp;</p>
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<h2 class="centerUpper blueOnGrey" style="margin-left: 2%; margin-right: 2%; margin-top: 80;"">Project Cornerstones</h2>
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<a href="https://2014.igem.org/Team:Waterloo/Deliver"><img src="https://static.igem.org/mediawiki/2014/9/98/Waterloo_deliver_icon.png" class="icon" /></a>
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<a href="https://2014.igem.org/Team:Waterloo/Silence"><img src="https://static.igem.org/mediawiki/2014/9/9e/Waterloo_silence_icon.png" class="icon" /></a>
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<a href="https://2014.igem.org/Team:Waterloo/Translate"><img src="https://static.igem.org/mediawiki/2014/7/7e/Waterloo_translate_icon.png" class="icon" /></a>
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<div style="text-align: center; width: 90%; margin: auto;"><p>Explore the three cornerstones of this year's project: <a href="https://2014.igem.org/Team:Waterloo/Deliver">delivering</a> the silencing <em>in vivo</em> using conjugation, <a href="https://2014.igem.org/Team:Waterloo/Silence">silencing</a> <em>mecA</em> expression using CRISPRi and sRNA and <a href="https://2014.igem.org/Team:Waterloo/Translate">translating</a> from the lab to the patients.</p></div>
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<h1 >WELCOME TO iGEM 2014! </h1>
 
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<b>2014 Project Description:<b>
 
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Waterloo’s 2014 iGEM project aims to remove antibiotic resistance in MRSA populations by using CRISPR and RNA interference systems that target mecA expression. The gene mecA in MRSA is responsible for it’s resistance against beta-lactam antibiotics. CRISPRi and RNAi systems can be incorporated onto a Staphylococcus conjugative plasmid, which would be able to replicate and transfer within a MRSA population, effectively disabling the antibiotic resistant phenotype in the population over time. A beta-lactam antibiotic can then be administered to kill the newly antibiotic-sensitive bacteria.
 
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To execute this idea, the project was divided into three milestones. The first milestone aims to model the ability of CRISPRi and RNAi to inhibit gene expression in the level one organism, Staphylococcus epidermidis. The second milestone aims to develop an effective conjugative plasmid in Staphylococcus to ensure a proper delivery mechanism for CRISPRi and/or RNAi. Measuring the effectiveness of the conjugative plasmid will allow us to apply our current mathematical models to determine the optimal time periods to apply the antibiotics to the population after the addition of the donor cells. The third milestone would be to evaluate and improve this system for MRSA populations.
 
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<p>Your team has been approved and you are ready to start the iGEM season!
 
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
 
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Waterloo&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
 
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<a href="https://2014.igem.org/Team:Waterloo"style="color:#000000">Home </a> </td>
 
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<a href="https://2014.igem.org/Team:Waterloo/Team"style="color:#000000"> Team </a> </td>
 
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Waterloo"style="color:#000000"> Official Team Profile </a></td>
 
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<a href="https://2014.igem.org/Team:Waterloo/Project"style="color:#000000"> Project</a></td>
 
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<a href="https://2014.igem.org/Team:Waterloo/Parts"style="color:#000000"> Parts</a></td>
 
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<a href="https://2014.igem.org/Team:Waterloo/Attributions"style="color:#000000"> Attributions </a></td>
 
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<p> Please be sure to keep these links, your audience will want to find your: </p>
 
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<li><a href="https://2014.igem.org/Team:Waterloo">Home</a> </li>
 
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<li><a href="https://2014.igem.org/Team:Waterloo/Team">Team</a> </li>
 
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Waterloo">Official Team Profile</a> </li>
 
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<li><a href="https://2014.igem.org/Team:Waterloo/Modeling">Modeling</a> </li>
 
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<li><a href="https://2014.igem.org/Team:Waterloo/Notebook">Notebook</a> </li>
 
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<li><a href="https://2014.igem.org/Team:Waterloo/Safety">Safety</a> </li>
 
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<p>There are a few wiki requirements teams must follow:</p>
 
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<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
 
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<li>All pages must be created under the team’s name space.</li>
 
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<li>As part of your documentation, keep the links from the menu to the left. </li>
 
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<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
 
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
 
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<tr><td colspan="3" > <h3> Tips  </h3></td></tr>
 
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<p>We are currently working on providing teams with some easy to use design templates.
 
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
 
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
 
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
 
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
 
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
 
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<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
 
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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Latest revision as of 02:33, 18 October 2014

Waterloo iGem 2014 Team

Staphylocide

Delivery of an Antibiotic Resistance Gene Silencing Mechanism to a MRSA Population using Bacterial Conjugation

Problem

MRSA is sweeping the nation, an antibiotic-resistant scourge migrating from hospitals into communities. We need a hero to fight it and Waterloo iGEM has designed a synthetic bacterium that just might do the trick...

Solution

...Staphylocide! Staphylocide delivers antibiotic resistance gene silencing in vivo and could one day be incorporated into a treatment that would help doctors defeat MRSA once and for all.

 

Project Cornerstones

Explore the three cornerstones of this year's project: delivering the silencing in vivo using conjugation, silencing mecA expression using CRISPRi and sRNA and translating from the lab to the patients.

Retrieved from "http://2014.igem.org/Team:Waterloo"