Team:Evry/Notebook

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<!--*************************************** WEEK XX ****************************************************************-->
 
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<h1 id="week2">Week 2: 28<sup>th</sup> July - 3<sup>th</sup> August</h1>
 
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<h2>Friday, 1th August</h2>
 
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<b><div align="center">Protocol for transformation </b><br/></div>
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We transformed TOP 10 E. coli with plasmid of conjugation. This plasmid contains erythromycin like resistance cassette. I plated them on LB-Agar with erythomycin antibiotic.
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<h2>Sunday, 3st August</h2>
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<b><div align="center">Transformed cells </b><br/></div>
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The cells transformed groth in the plate with erythromycin. I take one colonie in 3mL LB with erythromycin.
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<b><div align="center">Tests culture pseudovibrio with medium </b><br/></div>
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I take 1mL culture in M9 (aa and 3% NaCL)to 3 differents medium :<br/>
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9mL MB<br/>
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9mL M9<br/>
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9mL 2xYT<br/>
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9mL LB<br/>
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<h1 id="week1">Week 1: 4<sup>th</sup>August - 10<sup>rd</sup> August</h1>
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<h2>Monday, 4th August</h2>
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<b><div align="center">Tests culture pseudovibrio with medium </b><br/></div>
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MB : the cells growth<br/>
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M9 : the cells growth<br/>
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2xYT : the cells growth<br/>
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LB : the cells don't growth<br/>
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<b><div align="center">Plasmid DNA puriication </b><br/></div>
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A culture of E.coli transformed with plasmide of conjugation. A plasmid purification is realised with kit NucleoSpin.
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After that 94,5ng.L-1 is purified.
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<h2>Tuesday, 5th August</h2>
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A culture of DH5a pir transformed with pNK2 (plasmid use to the transposas) in LB with ampicillin growth.
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After that the plasmid s purified.
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Latest revision as of 17:24, 24 August 2014

IGEM Evry 2014

Notebook



Contacts

Retrieved from "http://2014.igem.org/Team:Evry/Notebook"