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- | <td colspan="2"><p class="info-18">In the modeling (Fig. 3-1-1-1.), the better mutualism between Company and Customer requires that the expression of LasI under the control of Prhl promoter (<a href="http://parts.igem.org/Part:BBa_R0071">BBa_R0071</a>) is the same level as the expression of RhlI under the control of Plux promoter (<a href="http://parts.igem.org/Part:BBa_R0062">BBa_R0062</a>). Thus, we firstly tested the expression level of Prhl promoter and Plux promoter through the reporter assay (Fig. 3-1-1-2.). </p></td> | + | <td colspan="2"><p class="info-18">In the modeling (Fig. 3-1-1-1), the better mutualism between Company and Customer requires that the expression of LasI under the control of Prhl promoter (<a href="http://parts.igem.org/Part:BBa_R0071">BBa_R0071</a>) is the same level as the expression of RhlI under the control of Plux promoter (<a href="http://parts.igem.org/Part:BBa_R0062">BBa_R0062</a>). Thus, we firstly tested the expression level of Prhl promoter and Plux promoter through the reporter assay (Fig. 3-1-1-2). </p></td> |
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| <strong>Fig. 3-1-1-1.</strong> The phase diagram of the intensity of Plux and Prhl promoter </div></td> | | <strong>Fig. 3-1-1-1.</strong> The phase diagram of the intensity of Plux and Prhl promoter </div></td> |
| <td width="488"><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_Plux_and.png"><img src="https://static.igem.org/mediawiki/2014/c/cf/Tokyo_Tech_Plux_and.png" width="500" height="190" align="middle" /></a></div> | | <td width="488"><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_Plux_and.png"><img src="https://static.igem.org/mediawiki/2014/c/cf/Tokyo_Tech_Plux_and.png" width="500" height="190" align="middle" /></a></div> |
- | <div align="center"><strong>Fig. 3-1-1-2.</strong> Plux and Prhl Reporter Assay flow chart</div></td> | + | <div align="center"><strong>Fig. 3-1-1-2.</strong> Plux and Prhl reporter assay flow chart</div></td> |
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- | <td colspan="2" class="info-18"><p class="info-18">Our purpose is to confirm the expression level of Prhl promoter (<a href="http://parts.igem.org/Part:BBa_R0071">BBa_R0071</a>) and Plux promoter (<a href="http://parts.igem.org/Part:BBa_R0062">BBa_R0062</a>). We prepared two plasmids sets shown in below (Fig. 3-1-2-1.). We measured fluorescence intensity by GFP expression when we added signaling molecules.</p> | + | <td colspan="2" class="info-18"><p class="info-18">Our purpose is to confirm the expression level of Prhl promoter (<a href="http://parts.igem.org/Part:BBa_R0071">BBa_R0071</a>) and Plux promoter (<a href="http://parts.igem.org/Part:BBa_R0062">BBa_R0062</a>). We prepared two plasmids sets shown in below (Fig. 3-1-2-1). We measured the fluorescence intensity by GFP expression when we added signaling molecules.</p> |
| <p class="info-18">We prepared four conditions shown in below.</p> | | <p class="info-18">We prepared four conditions shown in below.</p> |
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- | <td colspan="2"><div align="center"><strong>Fig. 3-1-2-1. </strong>Reporter plasmids </div> | + | <td colspan="2"><div align="center"><strong>Fig. 3-1-2-1. </strong>Reporter Plasmids </div> |
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| <div> </div> | | <div> </div> |
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- | <td colspan="2"><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_Fig3-1-4-1.png"><img src="https://static.igem.org/mediawiki/2014/d/d6/Tokyo_Tech_Fig3-1-4-1.png" width="400" height="122" /></a></div> | + | <td colspan="2"><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_Fig3-1-4-1.png"><img src="https://static.igem.org/mediawiki/2014/d/d6/Tokyo_Tech_Fig3-1-4-1.png" width="500"/></a></div> |
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- | <td colspan="2"><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_Fig3-1-4-2.png"><img src="https://static.igem.org/mediawiki/2014/3/37/Tokyo_Tech_Fig3-1-4-2.png" width="400" height="123" /> </a></div> | + | <td colspan="2"><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_Fig3-1-4-2.png"><img src="https://static.igem.org/mediawiki/2014/3/37/Tokyo_Tech_Fig3-1-4-2.png" width="500" /> </a></div> |
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| <p class="info-18"> | | <p class="info-18"> |
- | 11. Measure fluorescence intensity with a flow cytometer (We used BD FACSCaliburTM Flow Cytometer of Becton, Dickenson and Company). </p> | + | 11. Measure the fluorescence intensity with a flow cytometer (We used BD FACSCaliburTM Flow Cytometer of Becton, Dickenson and Company). </p> |
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