Team:Groningen

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Starting in 2008 the University of Groningen has attended the iGEM (international Genetically Engineered Machine) competition every year. This year the team of Groningen is making a bandage containing <i>Lactococcus lactis</i>. <i>L. Lactis</i> is generally known as the lactic acid producing bacterium used in the food industry such as in the production of yoghurt. This bacterium is able to protect the wound against several bacteria that can cause painful infections in burn wounds.
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Infections in burn wounds are currently only treatable with antibiotics. An increase in antibiotic resistance makes it harder every day to fight these bacteria. In our system <i>L. Lactis</i> produces a so-called lantibiotic, nisin. Nisin is effective against a group of gram-positive bacteria such as <i>Pseudomonas Aeruginosa</i> and resistance against nisin is hardly ever found and if found, the resistance does not last.
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Beside nisin the <i>L. lactis</i> will be able to produce the infection preventing molecules (IPMs) AiiA and DspB. AiiA will disrupt the communication mechanism of the harmful bacteria, this way the bacteria will not cause any trouble because it 'thinks' it is alone.  DspB is a molecule that prevents the harmful bacteria to form a layer (biofilm) on the wound. Additionally we want to try to make the bandage 'active' (producing nisin, DspB and AiiA) only when harmful bacteria are present in the wound. The bandage targets <i>Staphyolococcus Aureus</i> and <i>Pseudomonas Aeruginosa</i> specifically, two bacteria that are a problem in burn wound centres.
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The design of the bandage is important as well. <i>L. lactis</i> should not be able to get out of the bandage, but the IPMs should be able to reach the wound. Besides containing <i>L. Lactis</i> the bandage should allow sufficient oxygen to reach the wound.
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Finally, the whole package needs to be able to be stored for quite a while and still work. Therefore <i>L. lactis</i> will be stored as a powder and can be activated with water when the bandage is needed.
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We are also investigating the possibilities of having L. Lactis produce growth factors to aid in wound healing and to link the detection to the production of a chromoprotein to show when the bandage detects harmful bacteria.
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    <ul>
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      <li><a target="content" href="https://2014.igem.org/Team:Groningen/home"style="color:#000000">Home</a></li>
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      <li><a target="content" href="https://2014.igem.org/Team:Groningen/Team"style="color:#000000"> Team</a></li>
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      <li><a target="content" href="https://igem.org/Team.cgi?year=2014&team_name=Groningen"style="color:#000000"> Official Team Profile</a></li>
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      <li><a target="content" href="https://2014.igem.org/Team:Groningen/Project"style="color:#000000"> Project</a></li>
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      <li><a target="content" href="https://2014.igem.org/Team:Groningen/Parts"style="color:#000000"> Parts</a></li>
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      <li><a target="content" href="https://2014.igem.org/Team:Groningen/Modeling"style="color:#000000"> Modelling</a></li>
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      <li><a target="content" href="https://2014.igem.org/Team:Groningen/Notebook"style="color:#000000"> Notebook</a></li>
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      <li><a target="content" href="https://2014.igem.org/Team:Groningen/Safety"style=" color:#000000"> Safety </a></li>
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      <li><a target="content" href="https://2014.igem.org/Team:Groningen/Attributions"style="color:#000000"> Attributions </a></li>
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      <li><a target="content" href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="30px"></a></li>
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Latest revision as of 07:25, 16 October 2014

 
 
> LactoAid
 
 
Abstract
Background
 
 
> Project
 
 
The Bandage
Detection
Secretion
Toolbox
Parts
Model-Based Design
Characterization
Interlab Measurement Study
 
 
> Team
 
 
Members
Supervisors
Official Team Page
Acknowledgements
Sponsors
 
 
> Notebook
 
 
Secretion
Detection
The Bandage
Toolbox
Characterization
Vector
Protocols
 
 
> Policy & Practice
 
 
Events
Future Perspectives
Martini Hospital
Media Coverage
Collaboration
 
 
> Awards
 
 
Checklist
Prizes
 
 
> iGEM
 
 
 
 
 
 
 
 
 
Home > Overview
 
 
 
 
 
 
 
 
We are still looking for sponsors
 
 
We are still looking for sponsors
 
Please contact our acquisition team via Anna Lauxen if you want to sponsor us. You can reach her via annalauxen@igemgroningen.com.
 
 
 
 
Project
 
 
Project
 
Read everything about our project.
 
 
 
 
Building manual
 
 
Building manual
 
Problem making the paper toy on the flyer? Click here!
 
 
 
 
Abstract (Click to see all 11 languages):
 
 
Abstract (Click to see all 11 languages):
 
Infections caused by Staphylococcus aureus and Pseudomonas aeruginosa often pose problems for burn wound treatments. We developed a new kind of bandage that prevents these infections and reduces the use of antibiotics, thereby lowering the risk of developing antibiotic resistance. The bandage consists of a hydrogel that contains genetically engineered Lactococcus lactis with nutrients. The engineered strain of L. lactis detects the quorum sensing molecules of the two pathogens in the wound and subsequently produces the antimicrobial nisin as well as some other Infection-Preventing-Molecules (IPMs). These IPMs are the anti-biofilm protein Dispersin B and the quorum quenching protein AHLase. The gel is placed between two layers, a top layer to allow diffusion of gases and a bottom layer to contain the bacteria within the bandage. Hydrating the gel by breaking adjacent water pockets initiates the growth of the bacteria, thereby activating the bandage.
 
 
 
 
Policy and Practice
 
 
Policy and Practice
 
We reached out to the public and spread amazement and inspiration. Apart from that, we considered the ethical implications and future of our project and constructively thought about GMO regulation.
 
 
 
 
View our medal checklist
 
 
View our medal checklist
 
A quick link to the medal requirements we fulfilled.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
© 2014 iGem Groningen, Design: Randy Wind; Art: Andries de Vries, Freek de Wijs and Bente Lauxen; Photography: Bente Lauxen; Coding: Randy Wind and Anco Gietema.
 
 
 

Retrieved from "http://2014.igem.org/Team:Groningen"