Team:Oxford/how much can we degrade
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<div style="background-color:white; border-bottom-left-radius:10px;border-radius:10px; padding-left:10px;padding-right:10px;min-width:300px;margin-top:-50px;"> | <div style="background-color:white; border-bottom-left-radius:10px;border-radius:10px; padding-left:10px;padding-right:10px;min-width:300px;margin-top:-50px;"> | ||
- | <a href=" | + | <a href="https://static.igem.org/mediawiki/2014/3/3d/OxigemLAB_BOOK.pdf" target="_blank"><img src="https://static.igem.org/mediawiki/2014/5/50/OxigemLabbook.png" style="position:absolute;width:6%;margin-left:84%;margin-top:-13%;z-index:10;"></a> |
<a href="https://static.igem.org/mediawiki/2014/1/16/Oxigem_LAB_PROTOCOLS.pdf" target="_blank"><img src="https://static.igem.org/mediawiki/2014/a/a4/OxigemProtocols.png" style="position:absolute;width:6%;margin-left:91%;margin-top:-13%;z-index:10;"></a> | <a href="https://static.igem.org/mediawiki/2014/1/16/Oxigem_LAB_PROTOCOLS.pdf" target="_blank"><img src="https://static.igem.org/mediawiki/2014/a/a4/OxigemProtocols.png" style="position:absolute;width:6%;margin-left:91%;margin-top:-13%;z-index:10;"></a> | ||
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(what are Gompertz functions?)</a>. An example output growth curve of the model is shown here. | (what are Gompertz functions?)</a>. An example output growth curve of the model is shown here. | ||
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- | The scaling of the growth rate of the Gompertz function comes directly from growth curves of the DM4 bacteria that we obtained in the lab. | + | The scaling of the growth rate of the Gompertz function comes directly from growth curves of the DM4 bacteria that we obtained in the lab. |
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<div class="white_news_block2"> | <div class="white_news_block2"> | ||
<h1blue2>Using buffers to reduce the pH change of our system</h1blue2> | <h1blue2>Using buffers to reduce the pH change of our system</h1blue2> | ||
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+ | We have investigated the effect of using buffers in the aqueous part of our system.<br> | ||
+ | As a first approximation, we model our system of bacteria turning over DCM, producing HCl, as a chemical system in which HCl immediately enters the 'bulk' (extracellular) solution; in this system we have a single buffer (HEPES) to reduce the drop in pH, maximising the amount of DCM the entire system can degrade before the pH drops below a toxic level. | ||
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Derivation of the Van Slyke equation: | Derivation of the Van Slyke equation: | ||
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- | + | The numerical solution to this differential equation was confirmed by reducing the n interval by a factor of 100, which gave the same result. | |
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Latest revision as of 09:55, 15 January 2015