Team:Tokyo Tech/Judging Form

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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/C4HSL-dependent_3OC12HSL_production" style="width:400px; margin-left:-135px;">C4HSL-dependent 3OC12HSL production</a></li>
        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/C4HSL-dependent_3OC12HSL_production" style="width:400px; margin-left:-135px;">C4HSL-dependent 3OC12HSL production</a></li>
        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/3OC12HSL-dependent_C4HSL_production" style="width:400px; margin-left:-135px;">3OC12HSL-dependent C4HSL production</a></li>
        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/3OC12HSL-dependent_C4HSL_production" style="width:400px; margin-left:-135px;">3OC12HSL-dependent C4HSL production</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Symbiosis_confirmation_by_co-culture" style="width:400px; margin-left:-135px;">Mutualism Confirmation ~Co-culture Assay~</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Symbiosis_confirmation_by_co-culture" style="width:400px; margin-left:-135px;">Mutualism Confirmation ~Co-culture Assay~</a></li>
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  </ul>
  </ul>
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                 <tr>
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                   <td colspan="2"><div align="center" class="title-small">Judging Form  </div></td>
                   <td colspan="2"><div align="center" class="title-small">Judging Form  </div></td>
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                  <td colspan="2"><p class="info-18" style="text-indent:0px;">&gt; Original judging form is <a href="https://igem.org/2014_Judging_Form?id=1529">here</a>.</p></td>
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                   <td colspan="2"><h2>Parts Improvement</h2></td>
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                   <td colspan="2"><h2>Improved and New Parts</h2></td>
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                   <td colspan="2"><p class="info-18">We  construted new Prhl promoter by improving original Prhl promoter (<a href="http://parts.igem.org/Part:BBa_R0071">BBa_R0071</a>).</p></td>
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                   <td colspan="2"><p class="info-18">We  constructed new Prhl promoter by improving the original Prhl promoter (<a href="http://parts.igem.org/Part:BBa_R0071">BBa_R0071</a>).</p></td>
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                   <td colspan="2"><div align="center">Fig. 7-5-1. Fluorescence  intensity detected by flow cytometer</div></td>
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                   <td colspan="2"><div align="center"><strong>Fig. 7-5-1-1.</strong> Fluorescence  intensity detected by flow cytometer</div></td>
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                   <td colspan="2"><p class="info-18">By changing RhlR binding site and LuxR binding site, we  designed Prhl(RL) promoter (<a href="http://parts.igem.org/Part:BBa_K1529300">BBa_K1529300</a>),  Prhl(LR) promoter (<a href="http://parts.igem.org/Part:BBa_K1529310">BBa_K1529310</a>)  and Prhl(RR) promoter (<a href="http://parts.igem.org/Part:BBa_K1529320">BBa_K1529320</a>).</p></td>
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                   <td colspan="2"><p class="info-18">By changing RhlR binding site and LuxR binding site, we  designed Prhl(RL) promoter (<a href="http://parts.igem.org/Part:BBa_K1529300">BBa_K1529300</a>),  Prhl(LR) promoter (<a href="http://parts.igem.org/Part:BBa_K1529310">BBa_K1529310</a>)  and Prhl(RR) promoter (<a href="http://parts.igem.org/Part:BBa_K1529320">BBa_K1529320</a>).We think (BBa_K1529300) is a improved
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part and (BBa_K1529310) and  (BBa_K1529320) are new parts.We are sure that we made a big contribution to the iGEM and iGEMer by the
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construction of new Prhl promoter.</p></td>
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                   <td colspan="2"><p class="info-18">We think we made a big contribution to the iGEM and iGEMer  by the construction of new Prhl promoter.</p>
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                   <td colspan="2"><p class="info-18">Other new parts are Prhl(RL)-CmR-LasI(<a href="http://parts.igem.org/Part:BBa_K1529302">BBa_K1529302</a>) and Plux-CmR-RhlI(<a href="http://parts.igem.org/Part:BBa_K1529797">BBa_K1529797</a>
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).</p>
                   <p class="info-18" style="text-indent:0px;">&gt; Jump to <a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">Parts.</a> </p></td>
                   <p class="info-18" style="text-indent:0px;">&gt; Jump to <a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">Parts.</a> </p></td>
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                   <td colspan="2"><div align="center">Fig. 7-5-2. Our presentation at MUSE TALK</div></td>
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                   <td colspan="2"><div align="center"><strong>Fig. 7-5-2-1. </strong>Our presentation at MUSE TALK</div></td>
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                   <td colspan="2">&nbsp;</td>
                   <td colspan="2">&nbsp;</td>
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                  <td colspan="2"><p class="info-18" style="text-indent:0px;">&gt; Original judging form is <a href="https://igem.org/2014_Judging_Form?id=1529">here</a>.</p></td>
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               </table>
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               <p>&nbsp;</p>
               <p>&nbsp;</p>

Latest revision as of 03:05, 18 October 2014

Tokyo_Tech

Judging Form

What we have archived from this competition
   
Judging Form

> Original judging form is here.
   

Improved and New Parts

We constructed new Prhl promoter by improving the original Prhl promoter (BBa_R0071).
 
Fig. 7-5-1-1. Fluorescence intensity detected by flow cytometer
 

By changing RhlR binding site and LuxR binding site, we designed Prhl(RL) promoter (BBa_K1529300), Prhl(LR) promoter (BBa_K1529310) and Prhl(RR) promoter (BBa_K1529320).We think (BBa_K1529300) is a improved part and (BBa_K1529310) and (BBa_K1529320) are new parts.We are sure that we made a big contribution to the iGEM and iGEMer by the construction of new Prhl promoter.

Other new parts are Prhl(RL)-CmR-LasI(BBa_K1529302) and Plux-CmR-RhlI(BBa_K1529797 ).

> Jump to Parts.

 

Policy and Practice

Our team went beyond what is normally expected from iGEM teams in terms of policy & practices. We had much interactions to discuss modulate course of our project with the general public.

For example,　our team members and iGEM Tokyo_Tech 2013 veterans gave a presentation at a workshop called “MUSE TALK”. Managers, executives and technicians working in IT companies participated in this event to learn about things outside their specialty.

> Jump to Policy and Practices.

 
Fig. 7-5-2-1. Our presentation at MUSE TALK
 

After the presentation, many audiences were impressed with the technology of synthetic biology, and acknowledged it as a research which estimates future prospects.
 

 

 

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