Team:Glasgow/Parts

From 2014.igem.org

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</p>
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<a href="https://2014.igem.org/wiki/index.php?title=Team:Glasgow/Parts&action=edit">Click here to edit</a>
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<table id="biobricktable">
<table id="biobricktable">
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<th id="motBH" colspan="2"><a class="collectionlink" href="http://parts.igem.org/partsdb/uses.cgi?part=BBa_K1463750" target="_window">Contain MotB</a></th>
<th id="motBH" colspan="2"><a class="collectionlink" href="http://parts.igem.org/partsdb/uses.cgi?part=BBa_K1463750" target="_window">Contain MotB</a></th>
<th id="motAH" colspan="2"><a class="collectionlink" href="http://parts.igem.org/partsdb/uses.cgi?part=BBa_K1463700" target="_window">Contain MotA</a></th>
<th id="motAH" colspan="2"><a class="collectionlink" href="http://parts.igem.org/partsdb/uses.cgi?part=BBa_K1463700" target="_window">Contain MotA</a></th>
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<th id="fliCH" colspan="2"><a class="collectionlink" href="" target="_window">Contain FliC</a></th>
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<th id="fliCH" colspan="2"><a class="collectionlink" href="http://parts.igem.org/partsdb/uses.cgi?part=BBa_K1463600" target="_window">Contain FliC</a></th>
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<tr>
<tr>
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<td>BBa_K1463750 </td>
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<td><a href="http://parts.igem.org/Part:BBa_K1463750" target="_window">BBa_K1463750</a> </td>
<td>Basic MotB part</td>
<td>Basic MotB part</td>
<td>BBa_K1463700</td>
<td>BBa_K1463700</td>
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<tr>
<tr>
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<td>BBa_K1463751 </td>
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<td><a href="http://parts.igem.org/Part:BBa_K1463751" target="_window">BBa_K1463751</a> </td>
<td>Composite B0032, K1463750</td>
<td>Composite B0032, K1463750</td>
<td>BBa_K1463701 </td>
<td>BBa_K1463701 </td>
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<tr>
<tr>
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<td>BBa_K1463752 </td>
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<td><a href="http://parts.igem.org/Part:BBa_K1463752" target="_window">BBa_K1463752</a> </td>
<td>Composite J23100, B0032, K1463750</td>
<td>Composite J23100, B0032, K1463750</td>
<td>BBa_K1463702</td>
<td>BBa_K1463702</td>
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<td>BBa_K1463753 </td>
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<td><a href="http://parts.igem.org/Part:BBa_K1463753" target="_window">BBa_K1463753</a> </td>
<td>Composite J23103, B0032, K1463750</td>
<td>Composite J23103, B0032, K1463750</td>
<td>BBa_K1463703</td>
<td>BBa_K1463703</td>
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<tr>
<tr>
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<td>BBa_K1463754  </td>
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<td><a href="http://parts.igem.org/Part:BBa_K1463754" target="_window">BBa_K1463754</a> </td>
<td>Composite J23106, B0032, K1463750</td>
<td>Composite J23106, B0032, K1463750</td>
<td>BBa_K1463704 </td>
<td>BBa_K1463704 </td>
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<td>BBa_K1463755</td>
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<td><a href="http://parts.igem.org/Part:BBa_K1463755" target="_window">BBa_K1463755</a></td>
<td> Composite J23112, B0032, K1463750 </td>
<td> Composite J23112, B0032, K1463750 </td>
<td>BBa_K1463705 </td>
<td>BBa_K1463705 </td>
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<td>BBa_K1463756 </td>
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<td><a href="http://parts.igem.org/Part:BBa_K1463756" target="_window">BBa_K1463756</a> </td>
<td>Composite J23116, B0032, K1463750</td>
<td>Composite J23116, B0032, K1463750</td>
<td>BBa_K1463706 </td>
<td>BBa_K1463706 </td>
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<td colspan="2">BBa_K1463771 </td>
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<td colspan="2"><a href="http://parts.igem.org/Part:BBa_K1463771" target="_window" >BBa_K1463771</a> </td>
<td colspan="2">Composite J23112, B0032, K1463700, B0032, K1463750</td>
<td colspan="2">Composite J23112, B0032, K1463700, B0032, K1463750</td>
<td></td>
<td></td>
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</tr>
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<td colspan="2">BBa_K1463772  </td>
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<td colspan="2"><a href="http://parts.igem.org/Part:BBa_K1463772" target="_window" >BBa_K1463772</a> </td>
<td colspan="2">Composite J23106, B0032, K1463700, B0032, K1463750</td>
<td colspan="2">Composite J23106, B0032, K1463700, B0032, K1463750</td>
<td></td>
<td></td>
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<td colspan="2">BBa_K1463773  </td>
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<td colspan="2"><a href="http://parts.igem.org/Part:BBa_K1463773" target="_window" >BBa_K1463773</a> </td>
<td colspan="2">Composite J23116, B0032, K1463700, B0032, K1463750</td>
<td colspan="2">Composite J23116, B0032, K1463700, B0032, K1463750</td>
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<td></td>
<td></td>
<td></td>
<td></td>
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<td colspan="2">BBa_K1463760</td>
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<td colspan="2"><a href="http://parts.igem.org/Part:BBa_K1463760" target="_window">BBa_K1463760</a></td>
<td colspan="2">MotA and MotB with B0032 RBS</td>
<td colspan="2">MotA and MotB with B0032 RBS</td>
<td></td>
<td></td>
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<td colspan="2">BBa_K1463774</td>
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<td colspan="2"><a href="http://parts.igem.org/Part:BBa_K1463774" target="_window">BBa_K1463774</a></td>
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<td colspan="2">MotA and MotB under J23100 Promoter with BOO32 RBS.</td>
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<td colspan="2">MotA and MotB under J23100 Promoter with B0032 RBS.</td>
<td></td>
<td></td>
<td></td>
<td></td>
</tr>
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</table>
</table>
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<a class="editlink" href="https://2014.igem.org/wiki/index.php?title=Team:Glasgow/Parts&action=edit">Edit</a>
</div>
</div>

Latest revision as of 02:48, 18 October 2014

Bubble Test Page








Biobricks

The following table lists the biobricks we created. Many of these have additional characterisation information, available on their respective Registry pages.
We have also put together a Motility Parts Collection
Some important parts:

  • BBa_B0032 - Weak Ribosome Binding Site
  • BBa_B0034 - Strong Ribosome Binding Site
  • BBa_J61002 - Plasmid Vector
  • BBa_J23xxx - Promoter

Please note that all parts highlighted in RED have been submitted and received by the registry.

Biobrick Number Type Description Designer Length (BP) Design Notes
BBa_K1463000 Composite Switch Biobrick with RFP and GFP. BBa_K1463520, BBa_K1463560, BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0034, BBa_E0040 iGEM14_Glasgow 1744
BBa_K1463001 Composite Switch Biobrick with just GFP. BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0034, BBa_E0040 iGEM14_Glasgow 1013
BBa_K1463002 Composite Switch Biobrick with just GFP weaker RBS. BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0032, BBa_E0040 iGEM14_Glasgow 1014
BBa_K1463030 Basic Switch nucleotide spacer iGEM14_Glasgow 11
BBa_K1463040 Basic φC31 attP site iGEM14_Glasgow 54
BBa_K1463041 Basic φC31 attB site iGEM14_Glasgow 55
BBa_K1463050 Composite K1463040, K1463030, K1463501, B0010, K1463041 iGEM14_Glasgow 267
BBa_K1463100 GvpA RBS iGEM14_Glasgow 8
BBa_K1463205 Composite B0034, K737016 iGEM14_Glasgow 237
BBa_K1463207 Composite J61002, J23100, B0034, K737016 iGEM14_Glasgow 3226
BBa_K1463210 Composite K1463100, K737016 iGEM14_Glasgow 233
BBa_K1463225 Composite GvpC(K737017) and B0032 iGEM14_Glasgow 523
BBa_K1463340 Composite B0034, K737016, B0032, K737017 iGEM14_Glasgow 764
BBa_K1463345 Composite J61002, J23100, B0034, K737016, B0032, K737017 iGEM14_Glasgow 3767
BBa_K1463350 Composite K1463100, K737016, B0032, K737017 iGEM14_Glasgow 764
BBa_K1463400 φC31 Gp3 iGEM14_Glasgow 738
BBa_K1463401 Composite B0034, K1463400 iGEM14_Glasgow 756
BBa_K1463501 Promoter J23100 promoter in reverse iGEM14_Glasgow 38
BBa_K1463520 Basic E1010 RFP in reverse iGEM14_Glasgow 705
BBa_K1463560 Basic Strong reverse RBS iGEM14_Glasgow 10
BBa_K1463600 Basic FliC iGEM14_Glasgow 1497
BBa_K1463601 Composite B0034, K1463600 iGEM14_Glasgow 1515
BBa_K1463602 Composite J23116, B0032, B0034, K1463600 iGEM14_Glasgow 1579
BBa_K1463603 Composite J23106, B0032, B0034, K1463600 iGEM14_Glasgow 1579
BBa_K1463604 Composite J23100, B0032, B0034, K1463600 iGEM14_Glasgow 1579
BBa_K1463700 Basic MotA 888
BBa_K1463701 Composite B0032, K1463700 iGEM14_Glasgow 907
BBa_K1463702 Composite J23100, B0032, K1463700 iGEM14_Glasgow 950
BBa_K1463703 Composite J23103, B0032, K1463700 iGEM14_Glasgow 950
BBa_K1463704 Composite J23106, B0032, K1463700 iGEM14_Glasgow 950
BBa_K1463705 Composite J23112, B0032, K1463700 iGEM14_Glasgow 950
BBa_K1463706 Composite J23116, B0032, K1463700 iGEM14_Glasgow 950
BBa_K1463750 Basic MotB iGEM14_Glasgow 927
BBa_K1463751 Composite B0032, K1463750 iGEM14_Glasgow 946
BBa_K1463752 Composite J23100, B0032, K1463750 iGEM14_Glasgow 989
BBa_K1463753 Composite J23103, B0032, K1463750 iGEM14_Glasgow 989
BBa_K1463754 Composite J23106, B0032, K1463750 iGEM14_Glasgow 989
BBa_K1463755 Composite J23112, B0032, K1463750 iGEM14_Glasgow 989
BBa_K1463756 Composite J23116, B0032, K1463750 iGEM14_Glasgow 989
BBa_K1463770 Composite J23103, B0032, K1463700, B0032, K1463750 iGEM14_Glasgow 1904
BBa_K1463771 Composite J23112, B0032, K1463700, B0032, K1463750 iGEM14_Glasgow 1904
BBa_K1463772 Composite J23106, B0032, K1463700, B0032, K1463750 iGEM14_Glasgow 1904
BBa_K1463773 Composite J23116, B0032, K1463700, B0032, K1463750 iGEM14_Glasgow 1904
BBa_K1463805 Composite J61002, J23103, E1010 iGEM14_Glasgow 3703
BBa_K1463806 Composite J61002, J23106, E1010 iGEM14_Glasgow 3703
BBa_K1463807 Composite J61002, J23112, E1010 iGEM14_Glasgow 3703
BBa_K1463808 Composite J61002, J23116, E1010 iGEM14_Glasgow 3703

Motility Parts Collection

The motility parts collection is the culmination of our work on bacterial motility genes - MotA, MotB and FliC. Our project plan required that, for the bacteria to float, these genes would need switched off. Therefore, work had to be done to:

  • Knock out the genes from the bacteria
  • Be able to recover the motility
  • Make the parts suitable for placement on one side of the recombinase switch
Contain MotB Contain MotA Contain FliC
Part Number Description Part Number Description Part Number Description
BBa_K1463750 Basic MotB part BBa_K1463700 Basic MotA BBa_K1463600 The basic FliC part
BBa_K1463751 Composite B0032, K1463750 BBa_K1463701 Composite B0032, K1463700 BBa_K1463601 Composite B0034, K1463600
BBa_K1463752 Composite J23100, B0032, K1463750 BBa_K1463702 Composite J23100, B0032, K1463700 BBa_K1463602 Composite J23116, B0032, B0034, K1463600
BBa_K1463753 Composite J23103, B0032, K1463750 BBa_K1463703 Composite J23103, B0032, K1463700 BBa_K1463603 Composite J23106, B0032, B0034, K1463600
BBa_K1463754 Composite J23106, B0032, K1463750 BBa_K1463704 Composite J23106, B0032, K1463700 BBa_K1463604 Composite J23100, B0032, B0034, K1463600
BBa_K1463755 Composite J23112, B0032, K1463750 BBa_K1463705 Composite J23112, B0032, K1463700
BBa_K1463756 Composite J23116, B0032, K1463750 BBa_K1463706 Composite J23116, B0032, K1463700
Contain MotA and MotB
Part Number Description
BBa_K1463770 Composite J23103, B0032, K1463700, B0032, K1463750
BBa_K1463771 Composite J23112, B0032, K1463700, B0032, K1463750
BBa_K1463772 Composite J23106, B0032, K1463700, B0032, K1463750
BBa_K1463773 Composite J23116, B0032, K1463700, B0032, K1463750
BBa_K1463760 MotA and MotB with B0032 RBS
BBa_K1463774 MotA and MotB under J23100 Promoter with B0032 RBS.
Edit

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