Team:Heidelberg/Notebook/Materials
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Latest revision as of 20:49, 16 October 2014
MATERIALS
– and what else you might need ...
See below all the Materials we used. For the individual experiments visit our Notebook, the MidnightDoc, for a collection of our Methods please see Methods.
Kits
Kit | Supplier | Catalog Number |
---|---|---|
MinElute® PCR Purification Kit (250) | QIAGEN | 28006 |
Plasmid Plus Maxi Kit (25) | QIAGEN | 12963 |
Plasmid Plus Midi Kit (25) | QIAGEN | 12943 |
QIAEX II® Gel Extraction Kit (500) | QIAGEN | 20051 |
QIAGEN® Plasmid Plus Midi Kit (100) | QIAGEN | 12945 |
QIAquick® Gel Extraction Kit (250) | QIAGEN | 28706 |
QIAquick® PCR Purification Kit (250) | QIAGEN | 28106 |
QIAprep® Spin Miniprep Kit (250) | QIAGEN | 27106 |
QIAprep® Spin Miniprep Columns | QIAGEN | 27115 |
Protein Thermal Shift Starter Kit | 4462263 | Life Technologies |
EnzChek® Ultra Xylanase Assay Kit | Life Technologies | E33650 |
Marker
Marker | Supplier | Catalog Number |
---|---|---|
Quick-Load® 2-Log DNA Ladder (0.1-10.0 kb) | New England BioLabs | N3200S |
Quick-Load® 1 kb DNA Ladder | New England BioLabs | N0468S |
50 bp DNA Ladder | New England BioLabs | N3236S |
Gel loading solution | Sigma-Aldrich Chemie GmbH | G2526-5ML |
MagicMark XP Western Protein Standard | Life Technologies | LC5602 |
Novex® Sharp Pre-stained Protein Standard | Invitrogen | LC5800 |
SERVA Triple Color Protein Standard | SERVA Electrophoresis | 39258.01 |
Enzymes
Enzyme | Supplier | Catalog Number |
---|---|---|
DreamTaq Green PCR Master Mix (2X) | Thermo Fisher Scientific Biosciences GmbH | K1081 |
DreamTaq PCR MM | Fermentas Life Sciences | K1071 |
Gibson Assembly® Master Mix | New England Biolabs | E2611 S |
Lysozyme from Chicken Egg White | Sigma-Aldrich Chemie GmbH | L4919-500MG |
Phusion® Flash High-Fidelity PCR Master Mix | Biozym Scientific GmbH | F-548L |
Phusion® High-Fidelity PCR Master Mix | New England Biolabs | M0531 L |
T4 DNA Ligase | New England Biolabs GmbH | M0202 S |
2x PCR Master mix Solution (iTaq) | HISS DIAGNOSTICS GmbH | 25028 |
Sortase A (Staphylococcus aureus) | roboklon | E4400-01 |
TEV Protease | Th. Geyer GmbH | SA/T4455/000001 |
Xylanase, recombinant | Sigma-Aldrich Chemie GmbH | X2753-10G |
Human DNA (cytosine-5) Methyltransferase | NEB | M0230 S |
Antibodies
Antibody | Supplier | Catalog Number |
---|---|---|
Penta·His Antibody, BSA-free | QIAGEN | 34660 |
Anti-GFP | Roche | 11814460001 |
RFP antibody [5F8] | Chromotec | 5F8 |
Goat anti Mouse IgG | HRPO Dianova GmbH | 115-035-003 |
Rat IgG, HRP-linked whole antibody (from goat) | GE Healthcare | NA935 |
Restriction Enzymes
Enzyme | Supplier | Catalog Number |
---|---|---|
BamI | New England Biolabs | R3136 S |
BgIII | New England Biolabs | R0144 S |
BsaI-HF® | NEB | R3535 L |
BsmBI | NEB | R0580 L |
DpnI | New England Biolabs | R0176 S |
EcoRI | New England BioLabs | R0101S |
EcoRI-HF | New England Biolabs | R3101 |
HindIII-HF | New England Biolabs | R3104 S |
HpaII | NEB | R0171 S |
KpnI-HF | New England Biolabs | R3142 S |
MfeI-HF | New England Biolabs | R3589 S |
NheI-HF | New England BioLabs | R3131 S |
NotI-HF | New England BioLabs | R3189 S |
PacI | New England Biolabs | R0547 S |
PstI-HF | New England Biolabs | R3140 S |
PvuI-HF | New England BioLabs | R3150S |
SalI-HF | New England Biolabs | R3138 S |
Sau3AI | NEB | R0169 S |
SpeI-HF | New England BioLabs | R3133 S |
XbaI | New England BioLabs | R0145 S |
Bacterial Strains
Strain | Supplier | Catalog Number |
---|---|---|
E. coli DH10ß | New England Biolabs | C3019 |
E. coli Top10 | Invitrogen | C404010 |
E. coli Turbos | ||
E. coli MG1655 | ||
E. coli OneShot | ||
E. coli Bl21 (DE3) | ||
Bacillus subtilis | ||
Micrococcus lysodeikticus |
Antibiotics and Media Supplements
Antibiotic | Supplier | Catalog Number | Concentration stock solution | Dilution | Solvent |
---|---|---|---|---|---|
Ampicillin Anhydrous Crystalline | Sigma-Aldrich Chemie GmbH | A9393-5G | 100 mg/ml | 1:1000 | H2O |
Ampicillin Sodium Crystalline | Sigma-Aldrich Chemie GmbH | A9518-5G | 100 mg/ml | 1:1000 | H2O |
Chloramphenicol Crystalline | Sigma-Aldrich Chemie GmbH | C0378-5G | 30 mg/ml | 1:3000 | Ethanol |
Kanamycinsulfat Mixture of Componenta | Sigma-Aldrich Chemie GmbH | 60615-5G | 50 mg/ml | 1:1000 | H2O |
Tetracycline | Sigma-Aldrich Chemie GmbH | T7660 | 10 mg/ml | 1:1000 | Ethanol |
Propionic Acid Sodium Insect Cell*Culture | Sigma-Aldrich Chemie GmbH | P5436-100G | 100mM | 10mM | H2O |
Bacitracin | Sigma-Aldrich Chemie GmbH | B0125-50KU | - | - |
Media
Medium | Supplier | Catalog Number |
---|---|---|
SOC Outgrowth Medium | New England Biolabs GmbH | B9020 S |
LB BROTH BASE | Th. Geyer GmbH & Co KG | SA/L3022/001000 |
LB Broth Powder | Sigma-Aldrich Chemie GmbH | L3022-1KG |
M9 Minimal Salts | SERVA | 48505.01 |
Buffers
Buffer | Supplier | Catalog Number |
---|---|---|
NEBuffer Pack #4 (green) | New England Biolabs GmbH | B7004 S |
NEBuffer Pack #1 (yellow) | New England Biolabs GmbH | B7001 S |
NEBuffer Pack for T4 DNA Ligase | New England Biolabs GmbH | B0202 S |
NEBuffer Pack #2 (blue) | New England Biolabs GmbH | B7002 S |
NEBuffer Pack #3 (red) | New England Biolabs GmbH | B7003 S |
TAE - Buffer (50X) for Molecular Biology | VWR International GmbH | A4686.1000 |
Gel Loading Buffer | Sigma-Aldrich | G2526-5ML |
Tris Acetate-EDTA Buffer | Sigma-Aldrich | T9650-1L |
Other Chemicals
Chemical | Supplier | Catalog Number |
---|---|---|
Isopropyl B-D-Thiogalactopyranoside 1 piece | Sigma-Aldrich Chemie GmbH | I5502-1G |
Dimethyl Sulfoxide PCR Reagent | Sigma-Aldrich Chemie GmbH | D9170-1VL |
Glycerol Sigma Grade | Sigma-Aldrich Chemie GmbH | G9012-100ML |
5-Bromo-4-Chloro-3-Indolyl B-D-*Galactop | Sigma-Aldrich Chemie GmbH | B4252-100MG |
Bacteriological Agar | Sigma-Aldrich Chemie GmbH | A5306-250G |
L-Plus-Arabinose Crystalline | Sigma-Aldrich Chemie GmbH | A3256-25G |
Calciumchlorid Dihydrat | Th. Geyer GmbH & Co KG | SA/00223506/000500 |
Malt Extract from Starch Digestion | Sigma-Aldrich Chemie GmbH | M0383-100G |
D(+)-Saccharose, ACS, for Micro-Biology | Sigma-Aldrich Chemie GmbH | 84100-1KG |
Dimethyl Sulfoxide Plant Cell Culture*TE | D4540-100ML | |
Sodium Hydroxide Anhydrous Pellets | Th. Geyer GmbH & Co.KG | SA/S5881/000500 |
TRIZMA(R) Hydrochloride PH 3.5-5.0 | Sigma-Aldrich Chemie GmbH | T6666-50G |
L-Glutamine 200 MM Sterile | Sigma-Aldrich Chemie GmbH | G7513-20ML |
Ethanol 96% Denatured | Carl Roth GmbH & Co.KG | T171.3 |
Propanol-2 | Sigma-Aldrich Chemie GmbH | 59309-1L |
Natriumdodecylsulfat,SDS,99%, Ultra Pure | 13904 | |
Gold(III)-Chloride | Carl Roth GmbH & Co.KG | 5624.1 |
Pro-Leu | Sigma-Aldrich Chemie GmbH | P1130-1G |
Nitric Acid 65% p.a. Iso | Carl Roth GmbH & Co.KG | X943.1 |
Mops, Sodium | Sigma-Aldrich Chemie GmbH | M9024-25G |
L-Glutamine | Sigma-Aldrich Chemie GmbH | G7513-100ML |
Chelating Resin | Sigma-Aldrich Chemie GmbH | C7901-50G |
Potassium Hydroxide in Platellets | 6751.3 | |
Hydrochloric Acid 37% | 4625.1 | |
Pyruvic Acid | Sigma-Aldrich Chemie GmbH | 107360-25G |
Fmoc-Orn(BOC)-OH 96.0 % | 47560-5G-F | |
Glycerol >99.5% | Sigma-Aldrich Chemie GmbH | G9012-1L |
Water Molecular Biology Reagent | Sigma-Aldrich Chemie GmbH | W4502-1L |
Acetonitrile | Sigma-Aldrich Chemie GmbH | 34967-1L |
Ascorbic Acid 99% | Sigma-Aldrich Chemie GmbH | A92902-100G |
Imidazol 99% | Carl Roth GmbH | 3899.4 |
1,4-Dithiothreit p.a. 25g | Carl Roth GmbH | 6908.2 |
Phenylmethylsulfonylfluorid | Carl Roth GmbH | 6367.1 |
S-Adenosyl-L-Methionine Chloride (SAM) | Sigma-Aldrich Chemie GmbH | A7007-5MG |
Boric Acid | Carl Roth GmbH & Co.KG | 6943.1 |
Fluorecine Isothiocyanate Isomer | Sigma-Aldrich Chemie Gmb | F7250-50MG |
Hydroxylammonium chloride | Sigma-Aldrich Chemie GmbH | 159417-100G |
Rotiphorese® Gel 40 (19:1) 250 ml | Carl Roth GmbH | 3030.2 |
Rotiphorese® Gel 30 (37,5:1) 1 l | Carl Roth GmbH | 3029.1 |
Albumin Bovine Fraction V Powder | Sigma-Aldrich Chemie GmbH | A9647-50G |
Peptidoglycan from Micrococcusluteus | Sigma-Aldrich Chemie GmbH | 53243-10MG-F |
Adenosine 5´-Triphosphate (ATP) | NEB | P0756 S |
Electrophoresis
Reagent | Supplier | Catalog Number | Concentration | Solvent |
---|---|---|---|---|
Agarose Molecular Biology Reagent | Th. Geyer GmbH & Co KG | SA/A9539/000050 | 0.5% | H2O |
Agarose for Routine Use | Sigma-Aldrich Chemie GmbH | A9539-100G | - | - |
Gel Loading Dye Purple | NEB | B7024 S |
Primers and Oligos
Name | Sequence |
---|---|
VF2 | tgccacctgacgtctaagaa |
VR | attaccgcctttgagtgagc |
pSB1C3_F_SspDnaE-N-taataat | GACGCAGGTACAATTAAATAATAATACTAGTAGCGGCCGCTGCAG |
pSB1C3_R_Flag-SspDnaE_C | CTTTAACCTTGTCATCATCGTCTTTGTAGTCCATCTAGTACTTTCCTGTGTGACTCTAGAAGCG |
SspDnaE-C_F_pSB1C3-Flag | TACTAGATGGACTACAAAGACGATGATGACAAGGTTAAAGTTATCGGTCGTCGATCCC |
SspDnaE-C_R_BsaI-ccdB | CTCCCTTATACACAGCCAGTGGTCTCGAATTGTTTGTTAAAACAGTTGGCGGCGATAG |
ccdB_F_SspDnaE-C-BsaI | TTAACAAACAATTCGAGACCACTGGCTGTGTATAAGGGAGCCTGAC |
ccdB_R_BsaI-SspDnaE-N | TTAAAATTTCGGTACCAAAACTGAGGCAGCTAGTGAGACCCGCGTGGATCCGGCTTAC |
SspDnaE-N_F_ccdB-BsaI | GGTCTCACTAGCTGCCTCAGTTTTGGTACCG |
SspDnaE-N_R_taataat-pSB1C3 | GCGGCCGCTACTAGTATTATTATTTAATTGTACCTGCGTCAAGTAATG |
SspDnaE-C_R_6His-BsaI-ccdB | GCCAGTGGTCTCGAATTGATGGTGATGGTGATGGTGTTTGTTAAAACAGTTGGCGGCGATAG |
ccdB_F_SspDnaE-C-6His-BsaI | CAAACACCATCACCATCACCATCAATTCGAGACCACTGGCTGTGTATAAGGGAGCCTGAC |
pSB1C3_F_NpuDnaE-N | CGGGTTGATAATTTGCCGAATTAATAATACTAGTAGCGGCCGCTG |
pSB1C3_R_FLAG-NpuDnaE-C | CTTGTCGTCATCGTCTTTGTAGTCCATCTAGTACTTTCCTGTGTGACTCTAGAAGC |
NpuDnaE-C_F_pSB1C3-FLAG | AGGAAAGTACTAGATGGACTACAAAGACGATGACGACAAGCATATGATCAAAATAGCCACACG |
NpuDnaE-C_R_BsaI-ccdB | GCTCCCTTATACACAGCCAGTGGTCTCGAATTGATTGAAACAATTAGAAGCTATGAAGCC |
ccdB_F_NpuDnaE-C-BsaI | TTTCAATCAATTCGAGACCACTGGCTGTGTATAAGGGAGCC |
ccdB_R_BasI-NpuDnaE-N | CTTAAACAGCTAGTGAGACCCGCGTGGATCCGGCTTAC |
NpuDnaE-N_F_BsaI-ccdB | TAGTAAGCCGGATCCACGCGGGTCTCACTAGCTGTTTAAGCTATGAAACGGAAATATTG |
NpuDnaE-N_R_pSB1C3 | CCGCTACTAGTATTATTAATTCGGCAAATTATCAACCC |
NpuDnaE-C_R_6His-BsaI-ccdB | TGGTCTCGAATTGATGATGGTGATGGTGATGATTGAAACAATTAGAAGCTATGAAGCC |
ccdB_F_NpuDnaE-C-6His-BsaI | TGTTTCAATCATCACCATCACCATCATCAATTCGAGACCACTGGCTGTGTATAAGGGAGCC |
BBa_E0040_F_EcoR1 | GAAAGGAATTCATGCGTAAAGGAGAAGAACTTTTCACTGGAG |
BBa_E0040_R_Spe1 | TAGTAACTAGTTTTGTATAGTTCATCCATGCCATGTGTAATCC |
DNMT1_P1_R_deg.seq.731-1602 | GCATCCAGRTTGCTSCC |
DNMT1_P2_R_deg.seq.731-1602 | CCACCACACAGCATYTC |
DNMT1_P3_F_both_seq.731-1602 | ATGTGGGACCCGGCAGC |
DNMT1_P4_F_deg.seq.731-1602 | CAGTAYACCTTTCATGATGTG |
LOV-I539E_mut_F | TTTTGGTCTCAGAGGCCGCCGCCAAGCGGGTGAAGCTGGAC |
LOV-I539E_mut_R | ttttggtctcacctcattttctgcagttttcttaatcagcatg |
LOV-C450A_mut_F | ttttggtctcagccaggtttctacaaggtcctgaaactgatc |
LOV-C450A_mut_R | aaaaggtctcttggcgtttcttcccaaaatttcttcac |
T7RBS BioBrick_F | AAACTACGCTTTAGTAGCTTAATAACTCTGATAGTGCTAGTGTAGATCTC |
T7RBS BioBrick_R | PLACEHOLDER |
T7RBS-IGEMHD_F | PLACEHOLDER |
T7RBS-IGEMHD_R | PLACEHOLDER |
pSBX-T7fwd | AATTTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCGAATTCGCGGCCGCTT |
pSBX-T7rev | CTAGAAGCGGCCGCGAATTCGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTA |
T7RBS-IGEMHD_F_v2 | PLACEHOLDER |
T7RBS-IGEMHD_R_v2 | PLACEHOLDER |
T7RBS BioBrick_F_v2 | AATTCGCGGCCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATA |
T7RBS BioBrick_R_v2 | CTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTCTAGAAGCGGCCGCG |
Bba_E0040_F_BsaI=EcoR1 | GAAAGGGTCTCGAATTCATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTC |
Bba_E0040_R_His6_BsaI=Spe1 | TAGTAGGTCTCACTAGTGTGGTGATGGTGATGATGTTTGTATAGTTCATCCATGCCATGTG |
BsaI=EcoRI_null_BsaI=Spe1 | GCAGCAGGTCTCAAATTATGCGACTAGAGAGACCGCTGCT |
BsaI=SpeI_null_BsaI=EcoRI | AGCAGCGGTCTCTCTAGTCGCATAATTTGAGACCTGCTGC |
1F-smt3_pSB1A3 | AATTCGCGGCCGCTTCTAGATGTCGGACTCAGAAGTCAATCAAGAAGC |
2R-smt3_Tev_BsaI_ccdb | TCTCTACCCTGGAAGTACAGGTTTTCGGATCCACCAATCTGTTCTCTGTGAGC |
2F-ccdb_F_smt3_Tev_BsaI | AGATTGGTGGATCCGAAAACCTGTACTTCCAGGGTAGAGACCACTGGCTGTGTATAAGGGAGCC |
3R-ccdb_R-sortase-his6 | GCCGCTACTAGTATTATTAGTGATGGTGATGGTGATGACCACCGGTTTCCGGAAGTGAGACCCGCGTGGATCCGGCTTACTAAAAGCC |
3F-pSB1A3_F_ccdb-sortase-his6 | GGTCATCACCATCACCATCACTAATAATACTAGTAGCGGCCGCTGCAGTCCG |
1R-pSB1A3_smt3 | TTCTTGATTGACTTCTGAGTCCGACATCTAGAAGCGGCCGCGAATTCC |
ccdB_F_BsmBI | AGAGACGACTGGCTGTGTATAAGGGAGCCTGAC |
ccdB_R_BsmBI | TGAGACGCGCGTGGATCCGGCTTACTAAAAG |
mRFP_F_BsaI | AGAGACCCAATACGCAAACCGCCTCTC |
mRFP_R_BsaI | TGAGACCTATAAACGCAGAAAGGCCCACC |
T7RBS-IGEMHDv3_F | AATTCGCGGCCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGATCGGTGAAATGCACGACTGATAGTA |
T7RBS-IGEMHDv3_R | CTAGTACTATCAGTCGTGCATTTCACCGATCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTCTAGAAGCGGCCGCG |
NpuDnaEN_F | GTGGGCCTTTCTGCGTTTATAGGTCTCATGCTTAAGCTATGAAACGGAAATATTGACAG |
NpuDnaEN_R | GCTCCCTTATACACAGCCAGTCGTCTCTACCACCATTCGGCAAATTATCAACCCGCATC |
NpuDnaEC_F | GCCGGATCCACGCGCGTCTCAGGTGGAATCAAAATAGCCACACGTAAATATTTAGGCAAAC |
NpuDnaEC_R | GAGAGGCGGTTTGCGTATTGGGTCTCTGTTGGAAGCTATGAAGCCATTTTTGAGTGC |
SspDnaEN_F | PLACEHOLDER |
SspDnaEN_R | PLACEHOLDER |
SspDnaEC_F | PLACEHOLDER |
SspDnaEC_R | PLACEHOLDER |
AssemBIyIntN-linker_F | TGGTTAGTAGGGTGGC |
AssemBIyIntN-linker_R | ATTAGCCACCCTACTA |
AssemBIyIntC-linker_F | GATGGGTGGCGGTGGC |
AssemBIyIntC-linker_R | CACCGCCACCGCCACC |
SB-prep-3P-1 | gccgctgcagtccggcaaaaaa |
SB-prep-2Ea | atgaattccagaaatcatccttagcg |
pSBX_F_ccdB-BsmBI | GCCGGATCCACGCGCGTCTCATAATAAATCGGTGAAATGCACGACTGATAG |
pSBX_R_BsmBI-ccdB | GCTCCCTTATACACAGCCAGTCGTCTCTCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTC |
pSBX_F_mRFP-BsaI | GTGGGCCTTTCTGCGTTTATAGGTCTCATAATAAATCGGTGAAATGCACGACTGATAG |
pSBX_R_BsaI-mRFP | GAGAGGCGGTTTGCGTATTGGGTCTCTCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTC |
pSBX_F_taataa | TAATAAATCGGTGAAATGCACGACTGATAG |
pSBX_R_atg | CATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTC |
GFP_F_pSBX | TCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGATGCGTAAAGGAGAAGAACTTTTC |
GFP_R_pSBX | CTAGTACTATCAGTCGTGCATTTCACCGATTTATTATTTGTATAGTTCATCCATGCCATG |
Lambda-Lys_F_pSBX | TCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGGTAGAAATCAATAATCAACGTAAGGC |
Lambda-Lys_R_pSBX | CTAGTACTATCAGTCGTGCATTTCACCGATTTATTATACATCAATCTCTCTGACCGTTCCG |
1F-ccdb_F_pSB1A3-bsaI | ATTTCTGGAATTCGCGGCCGCTTCTAGATGTGAGACCACTGGCTGTGTATAAGGGAGCC |
1R-pSB1A3_R_bsaI-sortase-his-pSB1A3 | TTATACACAGCCAGTGGTCTCACATCTAGAAGCGGCCGCGAATTCC |
SspDnaX-S11C_F | TTAGTAAGCCGGATCCACGCGCGTCTCAGGTGGTGGCCTGCTGGTACACAACAGAGACCCAATACGCAAACCGCCTCTCCCC |
SspDnaX-S11C_R | GGGGAGAGGCGGTTTGCGTATTGGGTCTCTGTTGTGTACCAGCAGGCCACCACCTGAGACGCGCGTGGATCCGGCTTACTAA |
SspGyrB-S11C_F | TTAGTAAGCCGGATCCACGCGCGTCTCAGGTGGTGGTGTTTTCGTTCACAACAGAGACCCAATACGCAAACCGCCTCTCCCC |
SspGyrB-S11C_R | GGGGAGAGGCGGTTTGCGTATTGGGTCTCTGTTGTGAACGAAAACACCACCACCTGAGACGCGCGTGGATCCGGCTTACTAA |
TerDnaE3-S11C_F | TTAGTAAGCCGGATCCACGCGCGTCTCAGGTGGTGGTCTGATCGCGAGCAACAGAGACCCAATACGCAAACCGCCTCTCCCC |
TerDnaE3-S11C_R | GGGGAGAGGCGGTTTGCGTATTGGGTCTCTGTTGCTCGCGATCAGACCACCACCTGAGACGCGCGTGGATCCGGCTTACTAA |
NpuDnaEN_R_taataa-pSBX | CTAGTACTATCAGTCGTGCATTTCACCGATTTATTAATTCGGCAAATTATCAACCCGCATC |
NpuDnaEC_F_pSB-T7RBS | TCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGATCAAAATAGCCACACGTAAATATTTAGGC |
AssemBIyIntN-linker+H6_F | TGGTGGTCATCATCACCATCACCATTAG |
AssemBIyIntN-linker+H6_R | ATTACTAATGGTGATGGTGATGATGACC |
AssemBIyIntC-linker+H6_F | GATGCATCATCACCATCACCACGGT |
AssemBIyIntC-linker+H6_R | CACCACCGTGGTGATGGTGATGATG |
GFPn_1-157_R_KGG_TGCT_BsaI | CGTAGGTCTCGAGCACTTACCTCCTTTGTCTGCCATGATGTATACATTG |
GFPn_1-64_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCGAAAGTAGTGACAAGTGTTGGCC |
GFPn_1-64_1-157_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACCGTAAAGGAGAAGAACTTTTC |
GFPc_158-238_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACCAAAAGAATGGAATCAAAGTTAAC |
GFPc_65-238_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACGGTTATGGTGTTCAATGCTTTGC |
GFPc_65-238_158-238_R_H6_TAAT_BsaI | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGTTTGTATAGTTCATCCATGCCATGTG |
mCherryN_1-168_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCCTGCTTGATCTCGCCCTTC |
mCherryN_1-168_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACGTGAGCAAGGGCG |
mCherryC_169-236_R_H6_TAAT_BsaI | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGCTTGTACAGCTCGTCCATGC |
mCherryC_169-236_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACAGGCTGAAGCTGAAGGACG |
mRFPn_1-168_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCTTTCAGTTTCAGACGCATTTTGATTTC |
mRFPn_1-154_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCGTCTTCCGGGTACATACGTTCG |
mRFPn_1-154_1-168_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACGCTTCCTCCGAAGACG |
mRFPc_169-225_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACGACGGTGGTCACTACGACG |
mRFPc_155-225_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACGGTGCTCTGAAAGGTGAAATC |
mRFPc_155-225_169-225_R_H6_TAAT_BsaI | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGAGCACCGGTGGAGTG |
RenLucN_1-229_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCCTTAACGAGAGG |
RenLucN_1-229_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACGCTTCCAAGGTGTACG |
RenLucC_230-311_R_H6_TAAT_BsaI | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGTTACTGCTCGTTCTTCAGCACG |
RenLucC_230-311_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACAAGCCCGACGTCGTCC |
FireLucN_1-437_R_KGG_TGCT_BsaI | CGTAGGTCTCAAGCACTTACCTCCGCGGTCAACGATGAAG |
FireLucN_1-437_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACGAAGACGCCAAAAAC |
FireLucC_438-550_R_H6_TAAT_BsaI | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGCACGGCGATCTTTCC |
FireLucC_438-550_F_F_NWC_CAAC_BsaI | GCATGGTCTCACAACTGCTGGAACCTGAAGTCTCTGATTAAGTACAAAGGC |
PCA(t7rbs-npuc-mrfp-npun-igemhd)_F_prefix | CCCCGAATTCGCGGCCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATG |
PCA(t7rbs-npuc-mrfp-npun-igemhd)_R_SpeI | CGCTACTAGTACTATCAGTCGTGCATTTCACCG |
LambdaLys_F_BsaI_c-ext | CTGGTCTCACAACTGCTGGGAAGTAGAAATCAATAATCAACGTAAGGCGTTCC |
LambdaLys_R_HISTAG_n-ext_BsaI | CTGGTCTCTAGCATTTACCACGGCCGCTGTGATGATGATGATGATGGCTGCCTACATCAATCTCTCTGACCGTTCCG |
LambdaLys_R_RIGIDLINKER_n-ext_BsaI | CTGGTCTCTAGCATTTACCACGCGGTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCGCCACCTACATCAATCTCTCTGACCGTTCCG |
LambdyLys_R_FLEXLINKER_n-ext_BsaI | CTGGTCTCTAGCATTTACCACGTCCAGAACCACCACCAGAACCACCTACATCAATCTCTCTGACCGTTCCG |
Xyla_BB_F_RBS34_X | GCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTAGCACAGACTACTGGCAAAATTG |
Xyla_BB_R_SP | GGAAGCCTGCAGCGGCCGCTACTAGTATTATTACCACACTGTTACGTTAGAACTTCCAC |
Xyla_F_BsaI_C-ext | CTGGTCTCACAACTGCTGGGAAGCTAGCACAGACTACTGGCAAAATTG |
Xyla_F_BsaI_Destroyed-C-ext_His | CTGGTCTCACAACGGCTGGGAAGCTAGCACAGACTACTGGCAAAATTG |
Xyla_R_BsaI_N-ext | CTGGTCTCTAGCATTTACCACGCCACACTGTTACGTTAGAACTTCCAC |
Xyla_R_BsaI_N-ext_His | CTGGTCTCTAGCATTTACCACGATGATGGTGATGGTGATGCCACACTGTTACGTTAGAACTTCCAC |
2GateNinsert_F_X-T7RBS | CCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGAGAGACCCAATACGCAAACC |
2GateNinsert_R_SP | CCGGACTGCAGCGGCCGCTACTAGTATTTATTATGAGACGCGCGTGGATC |
2GateCinsert_F_X-T7RBS | CGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGAGAGACGACTGGCTGTGTATAAGG |
2GateCinsert_R_SP | CCGGACTGCAGCGGCCGCTACTAGTATTATTATGAGACCTATAAACGCAGAAAGG |
GFP^C_F_ | TATGGTGTTCAATGCTTTGCGAG |
GFP^C_R_H6 | GTGGTGATGGTGATGATGTTTGTATAGTTCATCCATGCCATGTG |
NpuDnaE^C_F_T7RBS-H6 | GGAGATACTAGATGCATCATCACCATCACCACATCAAAATAGCCACACGTAAATATTTAGGC |
NpuDnaE^C_R_GFP^C | CTCGCAAAGCATTGAACACCATAACAATTAGAAGCTATGAAGCCATTTTTGAGTG |
pSB_F_GFP^C-H6 | AACTATACAAACATCATCACCATCACCACTAATAATACTAGTAGCGGCCGCTGC |
pSB-T7RBS_R_H6 | GATGGTGATGATGCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTC |
GFP^N_F_T7RBS-H6 | GGAGATACTAGATGCATCATCACCATCACCACCGTAAAGGAGAAGAACTTTTCACTGGA |
GFP^N_R_NpuDnaE^N | CAAAGTAGTGACAAGTGTTGGCCATG |
NpuDnaE^N_F_GFP^N | TGGCCAACACTTGTCACTACTTTGTGTTTAAGCTATGAAACGGAAATATTGAC |
NpuDnaE^N_R_H6 | GGTGATGATGATTCGGCAAATTATCAACCCGCATC |
pSB_F_NpuDnaE^N-H6 | GATAATTTGCCGAATCATCATCACCATCACCACTAATAATACTAGTAGCGGCCGCTGC |
NpuDnaE^C_R_SN->AG-sfGFP^C | GCAAAGCACTGAACACCATAGCAACCAGCAGCTATGAAGCCATTTTTGAGTGC |
NpuDnaE^C_F_H6 | CATCATCATATCAAAATAGCCACACGTAAATATTTAGGC |
NpuDnaE^C_R_sfGFP | GCAAAGCACTGAACACCATAGCAATTAGAAGCTATGAAGCCATTTTTGAGTG |
sfGFP^C_F_NpuDnaE^C | GCTATGGTGTTCAGTGCTTTGCTC |
sfGFP^C_R_H6 | GTGGTGGTGGTGGTGTTTGTACAGTTCATCCATACCATGC |
pSB_F_H6-sfGFP^C | GTACAAACACCACCACCACCACCACTAATAATACTAGTAGCGGCCGCTGC |
pSB-T7RBS_R_NpuDnaE^C-H6 | CGTGTGGCTATTTTGATATGATGATGATGATGATGCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTC |
NpuDnaE^N_F_sfGFP^N | GACGCTGTGTTTAAGCTATGAAACGGAAATATTGACAGTAG |
NpuDnaE^N_R_H6 | GATGATTCGGCAAATTATCAACCCGCATC |
sfGFP^N_F_T7RBS-H6 | GAGATACTAGATGCACCACCACCACCACCACCGTAAAGGCGAAGAGCTGTTC |
sfGFP^N_R_NpuDnaE^N | CGTTTCATAGCTTAAACACAGCGTCGTTACCAGAGTCG |
pSB_F_NpuDnaE^N-H6 | GGGTTGATAATTTGCCGAATCATCATCATCATCATCATTAATAATACTAGTAGCGGCCGCTGCAG |
pSB-T7RBS_R_H6 | GTGGTGGTGGTGCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTC |
sfGFP^N_R_C->G-NpuDnaE^N | CATAGCTTAAACCCAGCGTCGTTACCAGAGTCG |
NpuDnaE^N_F_sfGFP-C>-G | CGACGCTGGGTTTAAGCTATGAAACGGAAATATTGACAGTAG |
pSBX1C3-Ter_F_KpnI | CTAGGGTCTACGTGATCGAGGTACCTACTAGAGCCAGGCATCAAATAAAA |
pSBX1C3-GFP_R_Ext_IntN_BamHI | GATCGAGGATCCCGCCAGAGAGATCAGAGAGTCACCAGAGATGCAACCAGATTCACGCAGTTTGTATAGTTCATCCATGCCATG |
BamHI-MTS_bs_minD-KpnI-**_F | GATCCCCTTTACAGGTGCTTGAAGAGCAAAACAAAGGAATGATGGCTAAGATTAAGTCATTTTTCGGAGTAAGATCTTAATAAGGTAC |
BamHI-MTS_bs_minD-KpnI-**_R | CTTATTAAGATCTTACTCCGAAAAATGACTTAATCTTAGCCATCATTCCTTTGTTTTGCTCTTCAAGCACCTGTAAAGGG |
SspDnaBC_F_M+H6 | GCCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGCATCATCACCATCACCACAGCACAGGAAAAAGAGTTTCTATTAAAGATT |
SspDnaBC_R_N->A+SGG | GCCGGACTGCAGCGGCCGCTACTAGTATTATTACCCGCCTGAGGCATGGACAATGATGTCATTGGC |
SspDnaBC_F_M-noH6 | GCCGCTTCTAGAGAATAATTTTGTTTAACTTTAAGAAGGAGATACTAGATGAGCACAGGAAAAAGAGTTTCTATTAAAGATT |
CPEC-GFP_F_MTS | GCAAAACAAAGGAATGATGGCTAAGATTAAGTCATTTTTCGGAGTAAGATCTAATACTAGAGCCAGGCATCAAATAAAACGAAAG |
CPEC-GFP_R_Next_Nint | GCCAGAGAGATCAGAGAGTCACCAGAGATGCAACCAGATTCACGCAGTTATTATTTGTATAGTTCATCCATGCCATGTGTAATC |
CPEC_GFP_Insert_oligo_F | CTCTGGTGACTCTCTGATCTCTCTGGCGCCTTTACAGGTGCTTGAAGAGCAAAACAAAGGAATGATGGCTAAGATTAAGT |
dnmt1insert_F_BsaI | ATGTTCGGTCTCAGGGTGGCGGTGGCAAAGATCGCATTAGCTGGCTGGGTCAG |
dnmt1insert_G_BsaI | ATGTTCGGTCTCATGCGCTGCTCAGCAGACACAGTTTAATTTC |
mRFP_F_BsmBIBsaI | ACTTACGGTCTCACGCAAGAGACGCAATACGCAAACCGCCTCTC |
mRFP_R_BsmBIBsaI | GCTTACGGTCTCAGAAGAGAGACGTATAAACGCAGAAAGGCCCACC |
LambdaLys_R_BsaI | CTGGTCTCTTACATCAATCTCTCTGACCGTTCCG |
ccdB_F_BsmBI-BsaI | CTCGTCTCATGTAAGAGACCACTGGCTGTGTATAAGGGAGCCTGAC |
ccdB_R_BsaI-BsmBI | CTCGTCTCAAGCAAGAGACCCGCGTGGATCCGGCTTACTAAAAG |
CPEC_GFP_Insert_oligo_R | ACTTAATCTTAGCCATCATTCCTTTGTTTTGCTCTTCAAGCACCTGTAAAGGCGCCAGAGAGATCAGAGAGTCACCAGAG |
Smt3_F_p2Zv2Npu_flag | GATGGACTACAAAGACGATGACGACAAGTCGGACTCAGAAGTCAATCAAGAAGC |
Smt3_R_p2Zv2Npu | GGCTATTTTGATGGATCCACCAATCTGTTCTCTGTGAG |
p2Zv2Npu_F_Smt3 | GGTGGATCCATCAAAATAGCCACACGTAAATATTTAGGC |
p2Zv2Npu_R_flag_Smt3 | CTTGTCGTCATCGTCTTTGTAGTCCATCTAGTATCTCCTTCTTAAAGTTAAACAAAATTATTCTC |
h6-linker_F | TGTAGGCAGCCATCATCATCATCATCACAGCGGCCGTGGTAAA |
h6-linker_R | AGCATTTACCACGGCCGCTGTGATGATGATGATGATGGCTGCC |
flex-linker_F | TGTAGGTGGTTCTGGTGGTGGTTCTGGACGTGGTAAA |
flex-linker_R | AGCATTTACCACGTCCAGAACCACCACCAGAACCACC |
rigid-linker_F | TGTAGGTGGCGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGCACCGCGTGGTAAA |
rigid-linker_R | AGCATTTACCACGCGGTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCGCCACC |
ccdB-pSBX4C5-mRFP(N)_F | AGAGACGACTGGCTGTGTATAAGGG |
ccdB-pSBX4C5-mRFP-(N)_R | TGAGACCTATAAACGCAGAAAGGC |
mRFP-pSBX4C5-ccdB-(C)_F | AGAGACCCAATACGCAAACCG |
mRFP-pSBX4C5-ccdB-(C)_R | TGAGACGCGCGTGGATC |
NpuDnaEN_F_C->G-BsaI-mRFP | GGCCTTTCTGCGTTTATAGGTCTCAGGCTTAAGCTATGAAACGGAAATATTGACA |
NpuDnaEN_R BsmBI_ccdB | CCCTTATACACAGCCAGTCGTCTCTACCACCATTCGGCAAATTATCAACCCG |
Npu DnaEC_F_BsmBI_ccdB | GATCCACGCGCGTCTCAGGTGGAATCAAAATAGCCACACGTAAATATTTAGG |
NpuDnaEC_R SN->AG-BsaI-mRFP | CGGTTTGCGTATTGGGTCTCTGCCTGCAGCTATGAAGCCATTTTTGAGTG |
Universal-S11N_F_BsaI_mRFP | GGCCTTTCTGCGTTTATAGGTCTC |
Universal-S11N_R BsmBI_ccdB | CCCTTATACACAGCCAGTCGTC |
Ssp DnaX-S11N_F_C->G_BsaI_mRFP | GGCCTTTCTGCGTTTATAGGTCTCAGGCTTAACCGGCGACAGCC |
Ssp DnaX-S11C_F_BsmBI_ccdB | GATCCACGCGCGTCTCAGGTGGTGGCCTGCTGGTA |
Ssp DnaX-S11C_R_BsaI_mRFP | CGGTTTGCGTATTGGGTCTCTGTTGTGTACCAGCAGGCCACCACC |
Ssp DnaX-S11C_R | CGGTTTGCGTATTGGGTCTCTGCCTGCTACCAGCAGGCCACCACC |
Ssp GyrB-S11N_F_C->G_BsaI_mRFP | GGCCTTTCTGCGTTTATAGGTCTCAGGCTTCAGCGGTGACACCCTG |
Ssp GyrB-S11C_F_BsmBI_ccdB | GATCCACGCGCGTCTCAGGTGGTGGTGTTTTCGTT |
Ssp GyrB-S11C_R_BsaI_mRFP | CGGTTTGCGTATTGGGTCTCTGTTGTGAACGAAAACACCACCACCTGAG |
Ssp GyrB-S11C_R_BsaI_mRFP | CGGTTTGCGTATTGGGTCTCTGCCTGCAACGAAAACACCACCACCTGAG |
Ter DnaE3-S11N_F_C->G_BsaI_mRFP | GGCCTTTCTGCGTTTATAGGTCTCAGGCTTAACTTACGAAACTGAGATCATGACC |
Ter DnaE3-S11C_F_BsmBI_ccdB | GATCCACGCGCGTCTCAGGTGGTGGTCTGATCGCG |
Ter DnaE3-S11C_R_BsaI_mRFP | CGGTTTGCGTATTGGGTCTCTGTTGCTCGCGATCAGACCACCACC |
Ter DnaE3-S11C_R | CGGTTTGCGTATTGGGTCTCTGCCTGCCGCGATCAGACCACCACC |
dnmt1insert_R_Flag_BsaI | GGTCTCAGAAGCTTGTCGTCATCGTCTTTGTAGTCTGCGCTGCTCAGCAGACACAG |
DNMT1_Substrate_R_1stMedC | GAAGCTGGGACTTCCGGGAGCTTC[5MedC]GATCTGAGAGTGCAA |
DNMT1_Substrate_F_1stMedC | TTGCACTCTCAGAT[5MedC]GGAAGCTCCCGGGAAGTCCCAGCTTC |
DNMT1_Substrate_F_2MedC | TTGCACTCTCAGAT[5MedC]GGAAGCTCC[5MedC]GGGAAGTCCCAGCTTC |
DNMT1_Substrate_F | TTGCACTCTCAGATCGGAAGCTCCCGGGAAGTCCCAGCTTC |
DNMT1_Substrate_R | GAAGCTGGGACTTCCGGGAGCTTCCGATCTGAGAGTGCAA |
DNMT1_Substrate_R_2MedC | GAAGCTGGGACTTC[5MedC]GGGAGCTTC[5MedC]GATCTGAGAGTGCAA |
VF2-C7T | TGCCACTTGACGTCTAAGAA |
sfGFP_T65C_F | CGACGCTGTGTTATGGTGTTCAGTGCTTTGCTC |
sfGFP_T65C_R | GAACACCATAACACAGCGTCGTTACCAGAGTCG |
rfp_F_BsaI | ACTTAACGCAAGAGACCCAATACGCAAACCGCCTCTCC |
rfp_R_BsaI | ACTTTCGAAGTGAGACCTATAAACGCAGAAAGGCCCACCC |
LiLaLy_F_BsaI | CTGGTCTCATAATAAATCGGTGAAATGCACGACTG |
LiLaLy_R_IasB | CTGGTCTCTTACATCAATCTCTCTGACCGTTCCG |
H6+exteins-insert_F | TGTAGGCAGCCATCATCATCATCATCACAGCGGCCGTGGTAAATGCTGGGAA |
H6+exteins-insert_R | ATTATTCCCAGCATTTACCACGGCCGCTGTGATGATGATGATGATGGCTGCC |
XylaLin_F_BsmBI | CTCGTCTCATAATAATACTAGTAGCGGCCGCTG |
XylaLin_R_IBmsB | CTCGTCTCTCCACACTGTTACGTTAGAACTTCCAC |
H6insert(Xyla)_F | GTGGGGCAGCCATCACCATCACCATCATAGTGGT |
H6insert(Xyla)_R | ATTAACCACTATGATGGTGATGGTGATGGCTGCC |
H6insert+exteins(Xyla)_F | GTGGCATCACCATCACCATCATCGTGGTAAATGCTGGGAA |
H6insert+exteins(Xyla)_R | ATTATTCCCAGCATTTACCACGATGATGGTGATGGTGATG |
H6Smt3_F_BBprefix | CGCTTCTAGATGGGCAGCAGCCATCATC |
mRFPexp_F_H6-TEVc | CACAGCAGCGGCGAAAACCTGTACTTCCAGGGTAGAGACCAATACGCAAACCGCCTCTC |
mRFPexp_F_Smt3-TEVc-BsaI | GTGGATCCGAAAACCTGTACTTCCAGGGTAGAGACCAATACGCAAACCGCCTCTC |
mRFPexp_R_BBsuffix-mockSortAc | GCTACTAGTATTATTAGGTTTCCGGAAGTGAGACCTATAAACGCAGAAAGGCCCACC |
pSB**3_F_mockSortAc | GGTCTCACTTCCGGAAACCTAATAATACTAGTAGCGGCCGCTGCAGTCC |
pSB**3_R_Smt3 | GCTGCTGCCCATCTAGAAGCGGCCGCGAATTC |
pSB**3_R_TEVc-H6 | GGTTTTCGCCGCTGCTGTGATGATGATGATGATGACTACTGCCCATCTAGAAGCGGCCGCGAATTC |
Smt3_R_TEVc-BsaI | CTGGAAGTACAGGTTTTCGGATCCACCAATCTGTTCTCTGTGAG |
mRFPexp_F_TEVc-BsaI | CAGTAGTGAGAATCTTTACTTCCAGGGTTGAGACCAATACGCAAACCGCCTCTCC |
mRFPexp_R_BBsuffix-H6-SortAc | CTAGTATTATTAGTGATGGTGATGGTGATGACCACCAGTTTCAGGAAGTGAGACCTATAAACGCAGAAAGGCCCACC |
pSB**3_R_TEVc-BsaI | CAACCCTGGAAGTAAAGATTCTCACTACTGCCCATCTAGAAGCGGCCGCGAATTCC |
eGFP_F_BsaI-G5RM | CAGAACGGTCTCAGGGTGGAGGAGGAGGAAGAATGGTGAGCAAGGGCGAGGAGC |
eGFP_R_BsaI | CAGAACGGTCTCAGAAGCTTGTACAGCTCGTCCATGCC |
ccdB_F_BsaI-BsmBI | CAGAACGGTCTCACGCATGAGACGACTGGCTGTGTATAAGGGAGCCTGAC |
ccdB_R_BsaI-BsmBI | CAGAACGGTCTCAGAAGTGAGACGCGCGTGGATCCGGCTTAC |
DNMT1lnk_0901/1f-FLAG_F | CGCAGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTGATTATAAAGATGATGATGATAAA |
DNMT1lnk_0901/1f-FLAG_R | CCAGTTTATCATCATCATCTTTATAATCACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACC |
DNMT1lnk_0825/1r-FLAG-p1F | CGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAA |
DNMT1lnk_0825/1r-FLAG-p1R | TTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGC |
DNMT1lnk_0825/1r-FLAG-p2F | AAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGCAGCAAGCGGAGCAGCAGCAGAAGCAGCAGCAAAAGATTATAAAGATGATGATGATAAA |
DNMT1lnk_0825/1r-FLAG-p2R | CCAGTTTATCATCATCATCTTTATAATCTTTTGCTGCTGCTTCTGCTGCTGCTCCGCTTGCTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGC |
EX-BsaI-A-NN-IasB-S_F | AATTCGCGGCCGCTTCTAGAGGTCTCCGATGCCTGCCTGCTAGAGACCTGTCTA |
EX-BsaI-A-NN-IasB-S_R | CTAGTAGACAGGTCTCTAGCAGGCAGGCATCGGAGACCTCTAGAAGCGGCCGCG |
EX-BsaI-CC-O-IasB-S_F | AATTCGCGGCCGCTTCTAGAGGTCTCCCAACCCTGCCTAATAGAGACCTGTCTA |
EX-BsaI-CC-O-IasB-S_R | CTAGTAGACAGGTCTCTATTAGGCAGGGTTGGGAGACCTCTAGAAGCGGCCGCG |
SsrA-Ctag-BsaI-Cext_BsaI_F | CAACTGCTGGGAAGGAGGAGGTGCTGCTAACGACGAAAACTACGCTCTGGCTGCT |
SsrA-Ctag-BsaI-Cext_BsaI_R | ATTAAGCAGCCAGAGCGTAGTTTTCGTCGTTAGCAGCACCTCCTCCTTCCCAGCA |
MTS_bs_minD_C_term_F | CAACTGCTGGGAAGGAGGAGGTCCTTTACAGGTGCTTGAAGAGCAAAACAAAGGAATGATGGCTAAGATTAAGTCATTTTTCGGAGTAAGATCT |
MTS_bs_minD_C_term_R | ATTAAGATCTTACTCCGAAAAATGACTTAATCTTAGCCATCATTCCTTTGTTTTGCTCTTCAAGCACCTGTAAAGGACCTCCTCCTTCCCAGCA |
myristoilation-Ntag_F | GATGGGCATCGGCAAGTCCAAGGGCGGAGGCAGGGGCAAG |
myristoilation-Ntag_R | AGCACTTGCCCCTGCCTCCGCCCTTGGACTTGCCGATGCC |
NLS-Ntag_F | GATGCCCAAGAAAAAGCGCAAGGTAGGCGGAGGCAGGGGCAAG |
NLS-Ntag_R | AGCACTTGCCCCTGCCTCCGCCTACCTTGCGCTTTTTCTTGGG |
OmpA_linker-BsaI_F | GGCCTTGGTCTCAGATGCATATGAAAGCTACTAAACTGGTACTGG |
OmpA_linker-BsaI_R | GGCCTTGGTCTCGAGCACTTGCCCCTAGAGCTCCCTCCTCCAGAAC |
pel_B-Ntag_F | GATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCCGGCGGAGGCAGGGGCAAG |
pel_B-Ntag_R | AGCACTTGCCCCTGCCTCCGCCGGCCATCGCCGGCTGGGCAGCGAGGAGCAGCAGACCAGCAGCAGCGGTCGGCAGCAGGTATTT |
PKI_NES-Ntag_F | GATGTTGGCTTTGAAATTGGCTGGTTTGGATATTGGCGGAGGCAGGGGCAAG |
PKI_NES-Ntag_R | AGCACTTGCCCCTGCCTCCGCCAATATCCAAACCAGCCAATTTCAAAGCCAA |
DNMT1_F_BsaI=CAAC-CWE | ATGTTCGGTCTCACAACTGCTGGGAAAAAGATCGCATTAGCTGGCTGGGTCAG |
ccdB_R_BsmBI=ATCA-H5SGRGK-TGCT=BsaI | CAGAACGGTCTCAAGCATTTACCACGGCCGCTGTGATGATGATGATGATTGAGACGCGCGTGGATCCGGCTTAC |
DMMT1_R_H6SGRGT-TGCT=BsaI | CAGAACGGTCTCAAGCAGGTACCACGGCCGCTGTGATGATGATGATGATGTGCGCTGCTCAGCAGACACAG |
SSpDnaBC_F_ccdB-CN | ATCCACGCGCGTCTCAGGTGGTAGCACAGGAAAAAGAGTTTCTATTAAAG |
SSpDnaBC_R_CC-mRFP | CGGTTTGCGTATTGGGTCTCTGTTGTGGACAATGATGTCATTGGC |
SSpDnaBC_R_CC*-mRFP | CGGTTTGCGTATTGGGTCTCTGCCTGCGACAATGATGTCATTGGC |
SspDnaBN_F_mRFP-NN | GCCTTTCTGCGTTTATAGGTCTCATGCTTATCTGGTGACTCTCTGATCTCTCTGG |
SspDnaBN_F_mRFP-NN* | GCCTTTCTGCGTTTATAGGTCTCAGGCTTATCTGGTGACTCTCTGATCTCTCTGG |
SspDnaBN_R_NC-ccdB | CCTTATACACAGCCAGTCGTCTCTACCACCACGGGACGCCAGAGAGATCAGAGAGTCACCAG |
CBD_His_BsaI_f | ATGTATGGTCTCAGATGCATCATCACCATCACCACGGTGGAGGTACCACAAATCCTGGTGTATC |
CBD_Nextein_BsaI_r | AAGTTCGGTCTCGAGCCACCAGATTCACGCAGTTGAAGCTGCCACAAGGC |
DNMT1_F_BsaI=GGGT-G | ATGTTCGGTCTCAGGGTGGTAAAGATCGCATTAGCTGGCTGGGTCAG |
AsLOV2-mutagenesis_middle_PstI_F | GAAACTGTAGGTTTCTACAAGGTCCTGAAAC |
AsLOV2_mutagenesis_middle_PstI_R | AGGACCTTGTAGAAACCTACAGTTTCTTCC |
ccdB_R_BsmBI=ATCA-H5SGRGT-TGCT=BsaI | CAGAACGGTCTCAAGCAGGTACCACGGCCGCTGTGATGATGATGATGATTGAGACGCGCGTGGATCCGGCTTAC |
DNMT1lnk_0901/1f-FLAG_R_v2 | GAAGTTTATCATCATCATCTTTATAATCACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACC |
DNMT1lnk_0915/4r-FLAG_p2F | AAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGCAAAAACCGCAGCAGCAGAAGCAGCAGCAAAAGATTATAAAGATGATGATGATAAA |
DNMT1lnk_0915/3f_p1F | CGCAGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGT |
DNMT1lnk_0915/3f_p1R | GCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACC |
DNMT1lnk_0915/3f_p2F | GGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCC |
DNMT1lnk_0915/3f_p2R | TGATGGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACC |
rigid+exteins-insert_F | TGTAGGTGGCGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGCACCGCGTGGTAAATGCTGGGAA |
rigid+exteins-insert_R | ATTATTCCCAGCATTTACCACGCGGTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCGCCACC |
DNMT1lnk_0915/4r-FLAG_p2R | GAAGTTTATCATCATCATCTTTATAATCTTTTGCTGCTGCTTCTGCTGCTGCGGTTTTTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGC |
DNMT1lnk_0915/2r_p1F | CGCAGCGGAAGCGGCGGCGAAAAACGTGCTGGCGGAAGCGGCGGCGAAAGAAGCGGCGGCGAAAGAAGCGGCGGCGA |
DNMT1lnk_0915/2r_p1R | TTCTTTCGCCGCCGCTTCTTTCGCCGCCGCTTCTTTCGCCGCCGCTTCCGCCAGCACGTTTTTCGCCGCCGCTTCCGC |
DNMT1lnk_0915/2r_p2F | AAGAAGCGGCGGCGAAAGAAGCGGCGGCGAAAGCGAACGTGCTGGCGGAAGCGGCGGCGAAAGAAGCGGCGGCGAAAC |
DNMT1lnk_0915/2r_p2R | TGATGTTTCGCCGCCGCTTCTTTCGCCGCCGCTTCCGCCAGCACGTTCGCTTTCGCCGCCGCTTCTTTCGCCGCCGC |
DNMT1lnk_0915/6f_p1F | CGCAGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGG |
DNMT1lnk_0915/6f_p1R | GCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACCGCTACCACCACC |
DNMT1lnk_0915/6f_p2F | TAGCGGTGGTGGTAGCGGTGGTGGTAGCGGTGGTGGTAGCGATTATAAAGATGATGATGATAAA |
DNMT1lnk_0915/6f_p2R | GAAGTTTATCATCATCATCTTTATAATCGCTACCACCACCGCTACCACCACCGCTACCACCACC |
DNMT1lnk_0915/1r_p1F | CGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAAAC |
DNMT1lnk_0915/1r_p1R | GCACGTTTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGC |
DNMT1lnk_0915/1r_p2F | GTGCTGGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAA |
DNMT1lnk_0915/1r_p2R | TTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCCA |
DNMT1lnk_0915/1r_p3F | AAGAAGCAGCAGCAAAAGCTAACGTGCTGGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAA |
DNMT1lnk_0915/1r_p3R | GAAGTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCCAGCACGTTAGCTTTTGCTGCTGC |
Backbone-H6_R | GATCCGTGGTGGTGGTGGTGG |
H6-Backbone_F | GGTGGTCATCATCATCATCATCATTAATAATACTAGTAG |
LOV-6_R_SspDnaXC | GCAGTTGTGTACCAGCAGGCCTTCTTTCGCCGCTTCATCAATATTTTGTGCAGTTTTCTTAATCAGCATGAC |
LOV-12_R_BsaIIasB | CGAGACCACCTTATTGGTCTCCAATATTTTGTGCAGTTTTCTTAATCAGCATGAC |
LOV-12_R_SspDnaXC | GCAGTTGTGTACCAGCAGGCCAATATTTTGTGCAGTTTTCTTAATCAGCATGAC |
sfGFPC_F_BsaIIasB | GGAGACCAATAAGGTGGTCTCGTGCTATGGTGTTCAGTGCTTTGC |
sfGFPC_F_SspDnaXC | GCCTGCTGGTACACAACTGCTATGGTGTTCAGTGCTTTGC |
sfGFPC_R_H6 | ATGATGATGATGATGATGACCACCTTTGTACAGTTCATCCATACCATGCG |
sfGFPN_F_H6 | CACCACCACCACGGATCACGTAAAGGCGAAGAGCTGTTC |
sfGFPN_R_SspDnaXN | CTGGCTGTCGCCGGTTAAGCACAGCGTCGTTACCAGAG |
sfGFPN_R_SspDnaXN* | CTGGCTGTCGCCGGTTAAACCCAGCGTCGTTACCAG |
SspDnaXN_F | TTAACCGGCGACAGCC |
SspDnaXC*-sfGFP_F_H6 | CACCACCACCACGGATCAGGCCTGCTGGTAGCTGGTTGCTATGGTGTTCAGTGCTTTGC |
SspDnaXC-sfGFP_F_H6 | CACCACCACCACGGATCAGGCCTGCTGGTACACAACTGCTATGGTGTTCAGTGCTTTGC |
SspDnaXN_R_H6 | ATGATGATGATGATGATGACCACCGTTTGCAACGAAG |
mRFP2-168_F_H6 | CACCACCACCACGGATCAGCTTCCTCCGAAGACGTTATC |
mRFP2-168_R_SspDnaXN | CTGGCTGTCGCCGGTTAAGCATTCGTCGATACCAGATCCTTTCAGTTTCAGACGCATTTTG |
mRFP2-168_R_SspDnaXN* | CTGGCTGTCGCCGGTTAAACCTTCGTCGATACCAGATCCTTTCAGTTTCAGACGCATTTTG |
mRFP169-225_F_-6-SspDnaXC-Ext | GCCTGCTGGTACACAACTGCCACACCGACGGTGGTCACTACGACGC |
mRFP169-225_F_-12-SspDnaXC-Ext-Jalpha | GCCTGCTGGTACACAACTGCCACACCGCGAACCTGGACGGTGGTCACTACGACGC |
mRFP169-225_F_BsaIIasB | GGAGACCAATAAGGTGGTCTCGGGTGGTCACTACGACGCTGAAG |
mRFP169-225_F_H6-SspDnaXC | CACCACCACCACGGATCAGGCCTGCTGGTACACAACTGCCACACCGACGGTGGTCACTACGACGC |
mRFP169-255_F_H6-SspDnaXC* | CACCACCACCACGGATCAGGCCTGCTGGTAGCTGGTTGCCACACCGACGGTGGTCACTACGACGC |
mRFP169-255_R_H6 | ATGATGATGATGATGATGACCACCAGCACCGGTGGAGTGACG |
LOV_F_H6 | CACCACCACCACGGATCATTGGCTACTACACTTGAACGTATTG |
GFPc*158-238_F_NWC_CAAC_BsaI | GCATGGTCTCAAGGCTGCTGGAACCAAAAGAATGGAATCAAAGTTAACTTC |
GFPc*_R_H6_TAAT=IasB | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGTTTGTATAGTTCATCCATGCCATG |
GFPn*1-157_R_KGG_GGCT=IasB | CGTAGGTCTCGAGCCCTTACCTCCTTTGTCTGCCATGATGTATACATTG |
GFPn*_F_H6_GATG_BsaI | GCATGGTCTCAGATGCATCATCACCATCACCACCGTAAAGGAGAAGAACTTTTCACTG |
mCherryC*169-236_F_BsaI=AGGC-NWC | GCATGGTCTCAAGGCTGCTGGAACAGGCTGAAGCTGAAGGACG |
mCherryC*_R_H6-TAAT=IasB | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGCTTGTACAGCTCGTCCATGC |
mCherryN*1-168_R_KGG-GGCT=IasB | CGTAGGTCTCAAGCCCTTACCTCCCTGCTTGATCTCGCCCTT |
mCherryN*_F_BsaI=GATG-H6 | GCATGGTCTCAGATGCATCATCACCATCACCACGTGAGCAAGGGCGAGG |
mRFPc*155-225_F_BsaI=AGGC-NWC | GCATGGTCTCAAGGCTGCTGGAACGGTGCTCTGAAAGGTGAAATC |
mRFPc*169-225_F_BsaI=AGGC-NWC | GCATGGTCTCAAGGCTGCTGGAACGACGGTGGTCACTACGACG |
mRFPc*_R_H6_TAAT=IasB | CGTAGGTCTCTATTAGTGGTGATGGTGATGATGAGCACCGGTGGAGTG |
mRFPn*1-154_R_KGG-GGCT=IasB | CGTAGGTCTCAAGCCCTTACCTCCGTCTTCCGGGTACATACGTTC |
mRFPn*1-168_R_KGG-GGCT=IasB | CGTAGGTCTCAAGCCCTTACCTCCTTTCAGTTTCAGACGCATTTTG |
mRFPn*_F_H6_BsaI=GATG | GCATGGTCTCAGATGCATCATCACCATCACCACGCTTCCTCCGAAGACGTTATC |
mRFP_F_BsmBI=GATG=IasB | AATTATCGTCTCAGATGAGAGACCCAATACGCA |
mRFP_R_BsaI=GGTG=IBmsB | TAATATCGTCTCACACCTGAGACCTATAAACGCAG |
mRFP_F_BsmBI=TGGT=IasB | AATTATCGTCTCATGGTAGAGACCCAATACGCAA |
mRFP_R_BsaI=TAAT=IBmsB | TAATATCGTCTCAATTATGAGACCTATAAACGCAGAAAGG |
bad1_F1 | TGTAGGTGGTGCAGAAGCAGCTGCTAAAGAAGCAGCAGCTAAAGTTAACCTGACCGCTGCTGAAGCT |
bad1_R1 | TGCTGCAGCTTCAGCAGCGGTCAGGTTAACTTTAGCTGCTGCTTCTTTAGCAGCTGCTTCTGCACCACC |
bad1_F2 | GCAGCAAAAGAAGCTGCAGCTAAAGTGAACCTGACCGCAGCAGAAGCTGCTGCAAAACGCGGTACC |
bad1_R2 | AGCAGGTACCGCGTTTTGCAGCAGCTTCTGCTGCGGTCAGGTTCACTTTAGCTGCAGCTTCTTT |
may1_F1 | TGTAGGTGGTGCAGAAGCTGCTGCTAAAGAAGCTGCTGCAAAAGCAGCAGCTGCACATCCGGAAGCTGCAGAAGCTGC |
may1_R1 | TTTGCTGCAGCTTCTGCAGCTTCCGGATGTGCAGCTGCTGCTTTTGCAGCAGCTTCTTTAGCAGCAGCTTCTGCACCACC |
may1_F2 | AGCAAAAGAAGCTGCAGCTAAAGCTAAAACCGCTGCTGAAGCTGCAGCAAAAGAAGCTGCAGCGAAAGCACGCGGTACC |
may1_R2 | AGCAGGTACCGCGTGCTTTCGCTGCAGCTTCTTTTGCTGCAGCTTCAGCAGCGGTTTTAGCTTTAGCTGCAGCTTCT |
sgt1_F | TGTAGGTGGTGCAGAAGCAGCTGCTAAAGAAGCTGCTGCAAAACGCGGTACC |
sgt1_R | AGCAGGTACCGCGTTTTGCAGCAGCTTCTTTAGCAGCTGCTTCTGCACCACC |
sgt2_F | TGTAGGTGGTGCTGAAGCTGCAGCAAAAGCTGCAGCACATCCGGAAGCTGCAGAAGCTGCAGCTAAACGCGGTACC |
sgt2_R | AGCAGGTACCGCGTTTAGCTGCAGCTTCTGCAGCTTCCGGATGTGCTGCAGCTTTTGCTGCAGCTTCAGCACCACC |
sho1_F | TGTAGGTGGTCGCGGTACC |
sho1_R | AGCAGGTACCGCGACCACC |
sho2_F | TGTAGGTGGTGCAGAAGCAGCTGCTAAACGCGGTACC |
sho2_R | AGCAGGTACCGCGTTTAGCAGCTGCTTCTGCACCACC |
LacI_F_VR | CAGCTCACTCAAAGGCGGTAATACTTTACGGCTAGCTCAGTCCTAGG |
LacI_R_pSB*C3 | GTTATCCCCTGATTCTGTGGATAACCTTATTACTGCCCGCTTTCCAGTC |
pSB*C3_F | GGTTATCCACAGAATCAGGGG |
pSB*C3_R | GTATTACCGCCTTTGAGTGAGC |
mRFP169-225_F_GSGIDECHT | GATCTGGTATCGACGAATGCCACACCGACGGTGGTCACTACGACGC |
mRFP169-225_F_GSGIDECHT-Jalpha | GATCTGGTATCGACGAATGCCACACCGCGAACCTGGACGGTGGTCACTACGACGC |
mRFP2-168_R_GSGIDECHT | GGTGTGGCATTCGTCGATACCAGATCCTTTCAGTTTCAGACGCATTTTG |
DNMT1lnk_0917/1r_p1F | CGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGTAAATC |
DNMT1lnk_0917/1r_p1R | GTAAGATTTACTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGC |
DNMT1lnk_0917/1r_p2F | TTACAGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAA |
DNMT1lnk_0917/1r_p2R | TTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCTGCT |
DNMT1lnk_0917/1r_p3F | AAGAAGCAGCAGCAAAAGCTGTAAATCTTACAGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAA |
DNMT1lnk_0917/1r_p3R | GAAGTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCTGCTGTAAGATTTACAGCTTTTGCTGCTGC |
DNMT1lnk_0917/2r_p1F | CGCAGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGTAAATCTTACAGCAGCAGAAG |
DNMT1lnk_0917/2r_p1R | GCTGCTTCTGCTGCTGTAAGATTTACTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGC |
DNMT1lnk_0917/2r_p2F | CAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGAAGCAGCAGCAAA |
DNMT1lnk_0917/2r_p2R | TTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTTTTGCT |
DNMT1lnk_0917/2r_p3F | AGAAGCAGCAGCAAAAGCAGTAAATCTTACAGCAGCAGAAGCAGCAGCAAAAGCAC |
DNMT1lnk_0917/2r_p3R | TGATGTGCTTTTGCTGCTGCTTCTGCTGCTGTAAGATTTACTGCTTTTGCTGCTGC |
mRFPexp_R_BsmBI=ATCA-H5SGRGT-TGCT=BsaI | CAGAACGGTCTCAAGCAGGTACCACGGCCGCTGTGATGATGATGATGATGGAGACGTATAAACGCAGAAAGGCCCACC |
mut-noPstIT-3A-GateNpuDnaE(C)SN->AG_F | GGCTTCATAGCAGCAGGCAGAGACCCAATAC |
mut-noPstIT-3A-GateNpuDnaE(C)SN->AG_R | CTGCCTGCTGCTATGAAGCCATTTTTGAGTG |
SspDnaE(N)_F_mRFP-NN | GCCTTTCTGCGTTTATAGGTCTCATGCTTAAGTTTTGGTACCGAAATTTTAACC |
SspDnaE(N)_R_NC-ccdB | CCTTATACACAGCCAGTCGTCTCTACCACCACGGGATTTAATTGTACCTGCGTCAAGTAATG |
SSpDnaE(C)_F_ccdB-CN | ATCCACGCGCGTCTCAGGTGGTGTTAAAGTTATCGGTCGTCGATC |
SSpDnaE(C)_R_CC-mRFP | CGGTTTGCGTATTGGGTCTCTGTTGGCGGCGATAGC |
univ#1long_F | TGTAGGTGGTGCAGAAGCAGCAGCTAAAGAAGCT |
univ#1long_R | AGCTGCAGCTTCTTTAGCTGCTGCTTCTGCACCACC |
univ#1short_F | TGTAGGTGGTGCAGAAGCTGCT |
univ#1short_R | CTTTGCAGCAGCTTCTGCACCACC |
univ#3_F | GCTGAAGCAGCTGCTAAAGAAGCAGCAGCTAAAGAAGCTGCTGCTAAAGAAGCTGCAGCTAAAGAAGCAGCTGCT |
univ#3_R | TGCCTTAGCAGCTGCTTCTTTAGCTGCAGCTTCTTTAGCAGCAGCTTCTTTAGCTGCTGCTTCTTTAGCAGCTGC |
bad2#2_F | GCAAAGGTGAACCTGACAGCT |
bad2#2_R | TTCAGCAGCTGTCAGGTTCAC |
bad2#4_F | AAGGCAAAAACCGCAGCTGAAGCTGCTGCAAAAGAAGCTGCAGCTAAAGAAGCTGCTGCAAAAGCTCGTGGTACC |
bad2#4_R | AGCAGGTACCACGAGCTTTTGCAGCAGCTTCTTTAGCTGCAGCTTCTTTTGCAGCAGCTTCAGCTGCGGTTTT |
bad3#2_F | GCAAAGGCATCTCTGCCGGCAGCT |
bad3#2_R | TTCAGCAGCTGCCGGCAGAGATGC |
bad3#4_F | AAGGCAAACGTGCTGGCAGAAGCAGCTGCTAAAGCACGTGGTACC |
bad3#4_R | AGCAGGTACCACGTGCTTTAGCAGCTGCTTCTGCCAGCACGTT |
bad4#2_F | GCAGCTAAAGAAGCTGCTGCTAAAGAAGCAGCTGCTAAAGAAGCAGCAGCTAAAGCTGTGAACCTGACCGCA |
bad4#2_R | TTCAGCTGCGGTCAGGTTCACAGCTTTAGCTGCTGCTTCTTTAGCAGCTGCTTCTTTAGCAGCAGCTTCTTT |
bad4#4_F | AAGGCAGTGAACCTGACCGCAGCTGAAGCTGCAGCTAAAGAAGCAGCTGCTAA |
bad4#4_R | GCTTCTTTAGCAGCTGCTTCTTTAGCTGCAGCTTCAGCTGCGGTCAGGTTCAC |
bad4#5_F | AGAAGCAGCTGCTAAAGAAGCAGCTGCTAAAGAAGCAGCTGCTAAAGCACGTGGTACC |
bad4#5_R | AGCAGGTACCACGTGCTTTAGCAGCTGCTTCTTTAGCAGCTGCTTCTTTAGCAGCT |
may2#2_F | GCAAAGGCTAAAACCGCTGCTGAAGCAGCAGCTAAAGAAGCAGCTGCGAAACGTGGTACC |
may2#2_R | AGCAGGTACCACGTTTCGCAGCTGCTTCTTTAGCTGCTGCTTCAGCAGCGGTTTTAGC |
may3#2_F | GCAGCTAAAGCTACCGGTGATCTGGCTGCAGAAGCAGCTGCAAAAGAAGCTGCTGCAAAAGCTCGCGGTACC |
may3#2_R | AGCAGGTACCGCGAGCTTTTGCAGCAGCTTCTTTTGCAGCTGCTTCTGCAGCCAGATCACCGGTAGCTTT |
ord1#2_F | GCAGCTAAAGCTACCGGTGATCTGGCTGCTGAAGCAGCAGCTAAAGCTGCACGTGGTACC |
ord1#2_R | AGCAGGTACCACGTGCAGCTTTAGCTGCTGCTTCAGCAGCCAGATCACCGGTAGCTTT |
ord2#2_F | GCAAAGGCTGCTGCAGCTGATGGTACCCTGGCAGAAGCTGCTGCTAAAGCAGCTCGTGGTACC |
ord2#2_R | AGCAGGTACCACGAGCTGCTTTAGCAGCAGCTTCTGCCAGGGTACCATCAGCTGCAGCAGC |
ord3#2_F | GCAGCTAAAGCTAGCCTGCCGGCTGCTGCAGAAGCTGCTGCAAAAGAAGCTGCAGCTAAACGTGGTACC |
ord3#2_R | AGCAGGTACCACGTTTAGCTGCAGCTTCTTTTGCAGCAGCTTCTGCAGCAGCCGGCAGGCTAGCTTT |
ord4#2_F | GCAGCTAAAGCTACCGGTGATCTGGCAGCAGAAGCTGCAGCTAAACGTGGTACC |
ord4#2_R | AGCAGGTACCACGTTTAGCTGCAGCTTCTGCTGCCAGATCACCGGTAGCTTT |
ord5#2_F | GCAAAGGCTGCTACCGGTGATCTGGCAGCAGAAGCAGCTGCTAAAGCACGTGGTACC |
ord5#2_R | AGCAGGTACCACGTGCTTTAGCAGCTGCTTCTGCTGCCAGATCACCGGTAGCAGC |
ord6#2_F | GCAAAGGCTAGCCTGCCGGCTGCAGCTGAAGCTGCAGCGAAAGAAGCAGCAGCTAAAGCTCGCGGTACC |
ord6#2_R | AGCAGGTACCGCGAGCTTTAGCTGCTGCTTCTTTCGCTGCAGCTTCAGCTGCAGCCGGCAGGCTAGC |
sgt3#2_F | GCAAAGGTGAACCTGACCGCTGCTGAAGCTGCAGCTAAACGTGGTACC |
sgt3#2_R | AGCAGGTACCACGTTTAGCTGCAGCTTCAGCAGCGGTCAGGTTCAC |
sgt4#2_F | GCAAAGGCTGCAGCACATCCGGAAGCTGCTGAAGCAGCTGCAAAAGCACGTGGTACC |
sgt4#2_R | AGCAGGTACCACGTGCTTTTGCAGCTGCTTCAGCAGCTTCCGGATGTGCTGCAGC |
sho3#2_F | GCAAAGGCTACCGGTGATCTGGCAGAAGCAGCTGCTAAACGTGGTACC |
sho3#2_R | AGCAGGTACCACGTTTAGCAGCTGCTTCTGCCAGATCACCGGTAGC |
rigid_RGT_F | TGTAGGTGGCGCAGAAGCAGCAGCAAAAGAAGCAGCAGCAAAAGCAGCACCGCGTGGTACC |
rigid_RGT_R | AGCAGGTACCACGCGGTGCTGCTTTTGCTGCTGCTTCTTTTGCTGCTGCTTCTGCGCCACC |
ccdB_R_BsmBI=ATCA-H5SGRGT-TGCT=BsaI | CAGAACGGTCTCAAGCAGGTACCACGGCCGCTGTGATGATGATGATGATTGAGACGCGCGTGGATCCGGCTTAC |
ccdB_R_BsmBI=ATCA-H5SGRGT-iGEMHD | CGTGCATTTCACCGATTTATTAGGTACCACGGCCGCTGTGATGATGATGATGATTGAGACGCGCGTGGATCCGGCTTAC |
Smt3_F_T7RBS | TTTGTTTAACTTTAAGAAGGAGATACTAGATGTCGGACTCAGAAGTCAATCAAGAAG |
DNMT1_F_Smt3-CWE | GTGGATGCTGGGAAAAAGATCGCATTAGCTGGCTGG |
Smt3_R_CWE-DNMT1 | TCTTTTTCCCAGCATCCACCAATCTGTTCTCTGTGAGC |
DNMT1_R_H6SGRGT-iGEMHD | CGTGCATTTCACCGATTTATTAGGTACCACGGCCGCTGTGATGATGATGATGATGTGCGCTGCTCAGCAGACAC |
DNMT1_R_RFC10suffix | TGCAGCGGCCGCTACTAGTATTATTATGCGCTGCTCAGCAGAC |
DNMT1/BB1_R_DNMT1/BB2 | CATACTGACCTGCTTGCAGAACACCAAAGG |
DNMT1/BB2_F_DNMT1/BB1 | GGTGTTCTGCAAGCAGGTCAGTATGGTGTTGC |
DNMT1/BB2_R_DNMT1/BB3 | CATCATGAAAGGTATATTGCAGTTTATGTGCAATAAC |
DNMT1/BB3_F_DNMT1/BB2 | GCACATAAACTGCAATATACCTTTCATGATGTG |
pSB**3_F_TAATAA | TAATAATACTAGTAGCGGCCGCTGCAGTCC |
Cas9CtrlSplit1_Fw | TGCTTCAACGGCGATAGCCTGCACGAG |
Cas9CtrlSplit1_Re | GTTGAAGCAGGACACCTGGGCTTTCTGG |
Cas9CtrlSplit2_Fw | GCCAGAGAGTACTGCTTCAACCAGACCACCCAGAAGG |
Cas9CtrlSplit2_Re | CTGGTTGAAGCAGTACTCTCTGGCCATTTCGATC |
Cas9Split1C_F_CFN_NpuDnaEC | CAACTGCTTCAACGGCGATAGTCTGCACGAGCAC |
Cas9Split1N_R_NpuDnaEN | CATAGCTCAAGCAGGACACCTGGGCTTTCTGGATG |
Cas9Split2C_F_CF_NpuDnaEC | CCAACTGCTTCAACCAGACCACCCAGAAGGG |
Cas9Split2N_R_Y_NpuDnaEN | CATAGCTCAAGCAGTACTCTCTGGCCATTTCGATC |
mCherry_Fw_BamHI | ATCGCGGATCCGGCACCATGGTGAGCAAGGGCGAGGAG |
mCherry_Rv_HindIII | GCGATAAGCTTCTACTTGTACAGCTCGTCCATGCCGC |
NpuDnaECSplit1&2C_F_NLS_Flag | AGACGATGACGATAAGCCAAAGAAGAAGCGGAAGGTCGGTGGTATCAAAATAGCCACACGTAAATATTTAGGC |
NpuDnaECSplit1C_R_CFN_Cas9Split1C | CTATCGCCGTTGAAGCAGTTGGAAGCTATGAAGCCATTTTTG |
NpuDnaECSplit2C_R_CF_Cas9Split2C | GTGGTCTGGTTGAAGCAGTTGGAAGCTATGAAGCCATTTTTG |
NpuDnaENSplit1&2N_R_NLS | CTCCGTGGATACCGACCTTCCGCTTCTTCTTTGGGGCACCACCATTCGGCAAATTATCAACCC |
NpuDnaENSplit1N_F_Cas9Split1N | CCAGGTGTCCTGCTTGAGCTATGAAACGG |
NpuDnaENSplit2N_Fw_Y_Cas9Split2N | GCCAGAGAGTACTGCTTGAGCTATGAAACGG |
PCDna3.1Split1&2C_R_Flag_NLS | CTTCTTTGGCTTATCGTCATCGTCTTTGTAATCCATGGTGGCGGATCCACTAG |
PCDna3.1Split1N_F_NLS | GAAGGTCGGTATCCACGGAGTCCCAGCAGCCTGAAAGCTTAAGTTTAAACCGCTGATCAGC |
PCDna3.1Split2N_F_NLS | GAAGGTCGGTATCCACGGAGTCCCAGCAGCCTGAAAGCTTAAGTTTAAACCGCTG |
FS_47_Cas9#49535_BamHI_fw | ATCGCGGATCCGCCACCATGGATTAC |
FS_48_Cas9#49535_HindIII_rv | GCGATAAGCTTTTTCTTTTTCTTAGCTTGACCAGC |
LOV-muta-PstI-middle2_F | GTGAAGAAATTTTGGGAAGAAACTGTAGGTTTCTACAAGGTCCTG |
LOV-muta-PstI-middle2_R | CAGTTTCAGGACCTTGTAGAAACCTACAGTTTCTTCCCAAAATTTC |
Plasmids
Name | Date | Brief Description | Genotype | Plasmid Map | GenBank-File |
---|---|---|---|---|---|
pJJDuet30 | https://www.addgene.org/11962/ | ||||
pSFBAD09 | https://www.addgene.org/11963/ | ||||
pHYRSF1 | https://www.addgene.org/34549/ | ||||
pHYRSF1-02 | https://www.addgene.org/24765/ | ||||
pGBMCS-SortA | https://www.addgene.org/21931/ |
Instruments
Instrument | Type | Manufacturer |
---|---|---|
Table Centrifuge | Microfuge® 18 Centrifuge | Beckman CoulterTM |
Table Centrifuge | Microfuge® 22R Centrifuge | Beckman CoulterTM |
Centrifuge | Allegra X-12R | Beckman CoulterTM |
Shaker | VORTEXGENIE 2 | Scientific Industries, SITM |
Heating plate (magnet stirrer) | MR Hei-Standard | Heidolph |
Heatblock | QBD4 | Grant |
Heatblock (shakeing function) | Thermomixer comfort | Eppendorf |
PCR-Machine | MyCyclerTM thermo cycler | BioRad |
PCR-Machine | T100 Therma Cycler | BioRad |
UV-Chamber | Transluminator | Vilber Lourmat |
Scale (fine) | PioneerTM PA114C | OHAUS |
Scale | PioneerTM PA4101C | OHAUS |
FACS | Cytomics FC 500 MPL | Beckman Coulter |
Fridge | KTP 1750 Premium | Liebherr |
Freezer | GP 1366 Premium | Liebherr |
Hood | Tischabzug | Wesemann® Laboreinrichtung |
Draw-Off Pump | Vacuhand control | Vacubrand |
Incubator | HT Multitron Version 2 | INFORS |
Plate Reader | Infinite M200 | Tecan |
Computer | Sun Microsystems | |
UV/VIS Spectrometer | Ultrospec 3300 pro | Amersham Biosciences |
Photometer | NanoDrop® ND-1000 Spectrophotometer | peQLab Biotechnologie GmbH |
Photometer | NanoVue | General Electric |
Ice Machine | MF22 | SCOTSMAN® |
Gel Electrophoresis Chamber | Mupid®-One | Advance |
Mini-PROTEAN Tetra Cell | 165-8001 | BioRad |
Cell Density Meter | Ultrospec10 | Amersham Biosciences |
PH-Meter | PH-Meter 765 Calimatic | Knick |
Incubator | Heraeus | Thermo Scientific |
Lyophilizer | Gefriertrocknungsanlage ALPHA 1-2 LD Plus Best. Nr. 101521 | Christ |
Ultra Sonification Stick | Sonoplus Gm 2070 (2002) | Bandelin |
Vacuum Manifold | Qiavac 24 Plus | QIAGEN |
Gas Cartridge Ventil CV470 | Burner | 13883 |
NuPAGE(R) Novex 3-8% Tris-Acetate Gel 1 | SDS-Gel | Life Technologies GmbH |
Lab Materials
Material | Name | Manufacturer |
---|---|---|
Bottle 50 ml, 100 ml, 200 ml, 500 ml, 1000 ml | ||
Beaker 50 ml, 100 ml, 200 ml, 500 ml 1000 ml | ||
Conical Flask 500 ml, 1000 ml, 100 ml | ||
Conical Flask 300 ml | ||
Single Channel Pipette | Pipetman® P2, P20, P200, P1000 | Gilson |
Multichannel Pipette | ||
Multistep Pipette | ||
Pipette | ||
Scalpels | Bayha | 13569 |
HISTRAP HP 5 X 1 ML, 5 EA | Th. Geyer GmbH | AM/17524701/000001 |
neoScrew-Microtubes 1,5 ml | NEOLAB GMBH | 290174576 |
CM SEPHADEX | Sigma-Aldrich Chemie GmbH | C50120-10G |
Dialysis Tubing | 1784.1 | Carl Roth GmbH |
Amicon Ultra 15ml 30K 8pk | UFC903008 | Merck Chemicals GmbH |
0.2 ml PCR Tube, Flat Cap, Natural | I1402-8200 | Starlab GmbH |
KLEENEX KIMWIPES | Kimberly-Clark | 4254 |
Microcentrifuge Tubes | ||
Centrifugetubes | ||
PCR 8-Strips | ||
Gloves Nitril Gr. L | Starlab powderfree | Starlab |
Gloves Nitril Gr. M | Starlab powderfree | Starlab |
Gloves Nitril Gr. S | Starlab powderfree | Starlab |
Filtertips | ||
Inoculating Loop | ||
Disposal Bags | ||
Flasks | ||
Plate | ||
Petri Dishes | P5731-500EA | Sigma-Aldrich Chemie GmbH |
Petri Dishes | N221.2 | Carl Roth GmbH & Co.KG |
Petri Dishes | 10558071 | Fischer Scientific |
TipOne® Pipette Tip | S1110-1700 | Starlab GmbH |
TipOne® Pipette Tip, 1000µl, Graduated | S1111-2721 | Starlab GmbH |
NeoBox-81 6er Set, je 1 x Transparent, g | 22916 | |
NeoLab-Marker for Reaction-Flaks | 19079 | |
Gene Pulser/MicroPulser Cuvettes, 0.1 cm | 165-2089 | |
NeoLab-Paper Scissors, 23 cm Long, Even | 23272 | |
TipOne® Pipette Tip, 200µl | S1110-1700 | Starlab GmbH |
TipOne® Pipette Tip, 10µl, Graduated, Re | S1111-3700 | Starlab GmbH |
Pipette, 5 ml | 606180 | Greiner bio-one GmbH |
Micro Plates, 96 well | 655101 | Greiner bio-one GmbH |
Ring out of Plumbum with Vinyl Coating, 57 mm In | 310161013 | NEOLAB GmbH |
Reaction Tube,S.L.1.5 ml,Colorless. | 12682 | Eppendorf, Fisher Scientific GmbH |
Reaction Tube,S.L.,2 ml, Colorless | 12776 | Eppendorf |
NeoTape-Writing Tape, 13 mm, Gray | 280126114 | NEOLAB GMBH |
NeoTape-Writing Tape, 25 mm, Salmon-Colored | 280126229 | NEOLAB GMBH |
10 ml Serological Pipette, Filter, Sterile | E4860-1011 | Starlab GmbH |
Gloves Latex + Alovera L | 14089 | |
Gloves Latex + Alovera M | 14088 | |
Gloves Latex + Alovera S | 14087 | |
CYTOONE 96W F-BTM TC PLT | CC7682-7596 | Starlab Gmbh |
PH-Stripes | WHAT10362000 | PANPEHA PLUS |
PCR Plates 96 well | GK96HIGH | Kisker Biotec |
100 Run24Barcode | 20110099-100 | GATC Biotech AG |
Adhesive PCR Film | 82-0558-A | Peqlab GmbH |
Tube Conical, Polypropylen, 50 ml | 352070 | NEOLAB GMBH |
Gene Pulser/MicroPulser Cuvettes, 0.1 cm | 165-2089 | Bio-Rad Laboratories GmbH |
0.2 ml 8-Tube Strips Without Caps, Nature | TBS-0201 | Bio-Rad Laboratories GmbH |
Round-Bottom Tubule, Polypropylen, 14 ml | 352059 | NEOLAB GMBH |
Optical Flat 8-Cap Strips | TCS-0803 | Bio-Rad Laboratories GmbH |
Inoculation Loop 10 µl, Blue, Sterile | 2900254437 | |
Corning Serological Pipette 50 ml | 14303 | |
Weighing Dish 500 ST | 1884.1 | Carl Roth GmbH & Co.KG |
Filter Paper | Z274836-1PAK | Sigma-Aldrich Chemie GmbH |
Inoculation Loop 10 µl | 2900254437 | NEOLAB GMBH |
Immobilon-PSQ Membran, PVDF | ISEQ00010 | Merck Milipore |
Further Recipies and Stocks
Ampicillin Stock Solution
Stock | Ampicillin 100 mg / ml |
Amount | 50 ml |
Storage | -24°C freezer |
Notes | Use in 1:1000 dilution |
Chloramphenicol Stock Solution
Stock | Chloramphenicol 30 mg / ml |
Amount | 10 ml |
Storage | -24°C freezer |
Notes | Solved in 100% ethanol. Use in 1:3000 dilution |
E.Coli BAP 1 Glycerol Stock
Stock | E. coli BAP1 glycerol stock |
Amount | 2 x 1.3 ml |
Storage | -80°C freezer |
E.Coli BAP1, Competent
Stock | E. coli BAP1 |
Amount | 18 x 100 µl |
Storage | -80°C freezer |
Notes | Grows extremely fast. Be careful with miniPreps, at least in cultures with ampicillin it tends to degrade all available ampicillin and then lose the respective plasmid. |
E. coli BAP1-pKD46 Glycerol Stock
What | E. coli BAP1-pKD46 glycerol stock (Ampr) |
Amount | 2 x 1.3 ml |
Storage | -80°C freezer |
Notes | Grow at 30°C only! Growth at 37°C will lead to loss of pKD46 plasmid. |
E.Coli BAP1-pLF03 Glycerol Stock
Stock | E. coli BAP1-pLF03 glycerol stock (Ampr) |
Amount | 1 x 1.3 ml |
Storage | -80°C freezer |
Notes | Might have low amount of plasmid-carrying bacteria due to long culturing time (all Amp in medium cleaved) |
E. Coli DH5a-pCP20 Glycerol Stock
Stock | E. coli DH5a-pCP20 glycerol stock (Ampr, Cmr) |
Amount | 2 x 1.3 ml |
Storage | -80°C freezer |
Notes | Grow at 30°C only! Growth at 37°C will lead to loss of pCP20 plasmid. |
Heat-Shock Competent E. Coli TOP10
Stock | E. coli TOP10, Heat-shock competent |
Amount | 200 x 100 µl |
Storage | -80°C freezer |
Notes | Verified on 2013-06-07. |
![](https://static.igem.org/mediawiki/2013/2/20/TOP10_test_2013-06-07.jpg)
E. Coli TOP10-BBa I746200/pSB1AK3 Glycerol Stock
Stock | E. coli TOP10-(BBa I746200 in pSB1AK3) (Ampr, Kanr) |
Amount | 1 x 1.3 ml |
Storage | -80°C freezer |
E. Coli TOP10-BBa J04450/pSB3C5 Glycerol Stock
Stock | E. coli TOP10-(BBa_J04450 in pSB3C5) glycerol stock (Cmr) |
Amount | 1 x 1.3 ml |
Storage | -80°C freezer |
E. Coli TOP10-pIK1 Glycerol Stock
Stock | E. coli TOP10-pIK1 glycerol stock (Cmr) |
Amount | 1 x 1.3 ml |
Storage | -80°C freezer |
E. Coli TOP10-pKD46 Glycerol Stock
Stock | E. coli TOP10-pKD46 glycerol stock (Ampr) |
Amount | 2 x 1.3 ml |
Storage | -80°C freezer |
Notes | Grow at 30°C only! Growth at 37°C will lead to loss of pKD46 plasmid. |
E. Coli TOP10-pMM65 Glycerol Stock
Stock | E. coli TOP10-pMM65 glycerol stock (Kanr) |
Amount | 1 x 1.3 ml |
Storage | -80°C freezer |
IPTG Stock Solution
Stock | IPTG 23.8 mg / ml |
Amount | 10 ml |
Storage | -20°C freezer |
Notes | Use 0.1 to 1 mM |
Kanamycin Stock Solution
Stock | Kanamycin 50 mg / ml |
Amount | 10 ml |
Storage | -24°C freezer |
Notes | Use in 1:1000 dilution |
M9 Medium
Name | M9 Minimal Salts 5x, Powder |
Amount | 1 l |
Storage | room temperature |
Notes | close tightly, hygroscopic |
- Add 200 ml of sterile M9 salt solution to 750 ml sterile, distilled H2O (45-50°C)
- Add sterile 20 ml 20% Glucose-solution, 2 ml 1 M MgSO4 and (optionally) 1 M CaCal
Reactivation Medium
for 1L
- 5.0 g Peptone
- 3.0 g Meat extract
- 1000.0 ml Distilled water