Team:Tokyo Tech/Judging Form

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<title>Tokyo_Tech</title>
<title>Tokyo_Tech</title>
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<img src="https://static.igem.org/mediawiki/2014/thumb/b/b7/Tokyo_Tech_Logo.png/613px-Tokyo_Tech_Logo.png" height="150" /></h1></div>
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        <div align="right"><img src="https://static.igem.org/mediawiki/2014/b/b4/Tokyo_Tech_Logo2.png" height="150" /></div>
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<a href="https://2014.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2014/thumb/8/84/Tokyo_Tech_iGEM_logo.png/794px-Tokyo_Tech_iGEM_logo.png" width="90" /></a></div>
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<li><a href="#">Experiment</a>
<li><a href="#">Experiment</a>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay" style="width:400px; margin-left:-135px;">Prhl reporter assay </a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Plux and Prhl reporter assay" style="width:400px; margin-left:-135px;">Plux and Prhl reporter assay</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay" style="width:400px; margin-left:-135px;">Improved Prhl reporter assay</a></li>
        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/C4HSL-dependent_3OC12HSL_production" style="width:400px; margin-left:-135px;">C4HSL-dependent 3OC12HSL production</a></li>
        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/C4HSL-dependent_3OC12HSL_production" style="width:400px; margin-left:-135px;">C4HSL-dependent 3OC12HSL production</a></li>
        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/3OC12HSL-dependent_C4HSL_production" style="width:400px; margin-left:-135px;">3OC12HSL-dependent C4HSL production</a></li>
        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/3OC12HSL-dependent_C4HSL_production" style="width:400px; margin-left:-135px;">3OC12HSL-dependent C4HSL production</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Symbiosis_confirmation_by_co-culture" style="width:400px; margin-left:-135px;">Symbiosis confirmation by co-culture </a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Symbiosis_confirmation_by_co-culture" style="width:400px; margin-left:-135px;">Mutualism Confirmation ~Co-culture Assay~</a></li>
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        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Transporter_assay" style="width:400px; margin-left:-135px;">Transporter assay</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling">Modeling</a></li>
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<li><a href="#">Modeling</a>
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                          <ul>
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                              <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Overview"  style="width:400px; margin-left:-135px;">Overview</a></li>
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                              <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Growth Conditions For Company And Customer"  style="width:400px; margin-left:-135px;">Growth Conditions For Company And Customer</a></li>
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                              <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Analysis of C4HSL-dependent Switch" style="width:400px; margin-left:-135px;">Analysis of C4HSL-dependent Switch</a></li>
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                              <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Economic Wave"  style="width:400px; margin-left:-135px;">Economic Wave</a></li>
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                          </ul>
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                        </li>
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">Parts</a></li>
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">Parts</a></li>
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices" style="height:50px; padding-top:3px;">Policy and Practices</a></li>
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices" style="height:50px; padding-top:3px;">Policy and Practices</a></li>
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<h2 class="title">Judging Form</h2>
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      <h2 class="title">Judging Form</h2>
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                <span class="meta">What we have done</span>
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            <span class="meta">What we have archived from this competition</span>
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<p>Under Construction！</p>
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<p>&nbsp;</p>
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              <div id="gototop"><a href="#"><img src="https://static.igem.org/mediawiki/2014/5/55/Tokyo_Tech_Go-to-top-icon.png" height="50" /></a></div>
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                  <td width="445">&nbsp;</td>
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<div align="center"><img src="http://www.actmp2014.com/images/under_construction%20(1).png" /></div>
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                  <td width="445">&nbsp;</td>
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                  <td colspan="2"><div align="center" class="title-small">Judging Form  </div></td>
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                  <td colspan="2"><p class="info-18" style="text-indent:0px;">&gt; Original judging form is <a href="https://igem.org/2014_Judging_Form?id=1529">here</a>.</p></td>
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                </tr>
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                  <td>&nbsp;</td>
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                  <td>&nbsp;</td>
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                  <td colspan="2"><h2>Improved and New Parts</h2></td>
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                  <td colspan="2"><p class="info-18">We  constructed new Prhl promoter by improving the original Prhl promoter (<a href="http://parts.igem.org/Part:BBa_R0071">BBa_R0071</a>).</p></td>
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                  <td colspan="2">&nbsp;</td>
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                  <td colspan="2"><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_2-1-9.png"><img src="https://static.igem.org/mediawiki/2014/6/6f/Tokyo_Tech_2-1-9.png" width="500"  /></a></div></td>
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                  <td colspan="2"><div align="center"><strong>Fig. 7-5-1-1.</strong> Fluorescence  intensity detected by flow cytometer</div></td>
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                  <td colspan="2">&nbsp;</td>
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                </tr>
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                  <td colspan="2"><p class="info-18">By changing RhlR binding site and LuxR binding site, we  designed Prhl(RL) promoter (<a href="http://parts.igem.org/Part:BBa_K1529300">BBa_K1529300</a>),  Prhl(LR) promoter (<a href="http://parts.igem.org/Part:BBa_K1529310">BBa_K1529310</a>)  and Prhl(RR) promoter (<a href="http://parts.igem.org/Part:BBa_K1529320">BBa_K1529320</a>).We think (BBa_K1529300) is a improved
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part and (BBa_K1529310) and  (BBa_K1529320) are new parts.We are sure that we made a big contribution to the iGEM and iGEMer by the
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construction of new Prhl promoter.</p></td>
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                </tr>
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                <tr>
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                  <td colspan="2"><p class="info-18">Other new parts are Prhl(RL)-CmR-LasI(<a href="http://parts.igem.org/Part:BBa_K1529302">BBa_K1529302</a>) and Plux-CmR-RhlI(<a href="http://parts.igem.org/Part:BBa_K1529797">BBa_K1529797</a>
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).</p>
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                  <p class="info-18" style="text-indent:0px;">&gt; Jump to <a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">Parts.</a> </p></td>
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                  <td colspan="2">&nbsp;</td>
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                  <td colspan="2"><h2>Policy and Practice</h2></td>
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                  <td colspan="2"><p class="info-18">Our  team went beyond what is normally expected from iGEM teams in terms of policy  &amp; practices. We had much interactions to discuss modulate course of our  project with the general public.</p></td>
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                </tr>
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                <tr>
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                  <td colspan="2"><p class="info-18">For  example,　our team members and iGEM Tokyo_Tech 2013 veterans gave a  presentation at a workshop called “MUSE TALK”.  Managers, executives and technicians working in IT companies  participated in this event to learn about things outside their specialty.</p>
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                  <p class="info-18" style="text-indent:0px;">&gt; Jump to <a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices">Policy and Practices. </a></p></td>
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                  <td colspan="2">&nbsp;</td>
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                  <td colspan="2"><div align="center"><a href="https://2014.igem.org/File:Tokyo_Tech_MUSE1.jpg"><img src="https://static.igem.org/mediawiki/2014/d/d3/Tokyo_Tech_MUSE1.jpg" width="500"></a></div></td>
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                  <td colspan="2"><div align="center"><strong>Fig. 7-5-2-1. </strong>Our presentation at MUSE TALK</div></td>
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                  <td colspan="2">&nbsp;</td>
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                  <td colspan="2"><p class="info-18">After the presentation, many audiences were impressed with  the technology of synthetic biology, and acknowledged it as a research which  estimates future prospects.</p></td>
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                  <td colspan="2">&nbsp;</td>
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Latest revision as of 03:05, 18 October 2014

Tokyo_Tech

Judging Form

What we have archived from this competition
   
Judging Form

> Original judging form is here.
   

Improved and New Parts

We constructed new Prhl promoter by improving the original Prhl promoter (BBa_R0071).
 
Fig. 7-5-1-1. Fluorescence intensity detected by flow cytometer
 

By changing RhlR binding site and LuxR binding site, we designed Prhl(RL) promoter (BBa_K1529300), Prhl(LR) promoter (BBa_K1529310) and Prhl(RR) promoter (BBa_K1529320).We think (BBa_K1529300) is a improved part and (BBa_K1529310) and (BBa_K1529320) are new parts.We are sure that we made a big contribution to the iGEM and iGEMer by the construction of new Prhl promoter.

Other new parts are Prhl(RL)-CmR-LasI(BBa_K1529302) and Plux-CmR-RhlI(BBa_K1529797 ).

> Jump to Parts.

 

Policy and Practice

Our team went beyond what is normally expected from iGEM teams in terms of policy & practices. We had much interactions to discuss modulate course of our project with the general public.

For example,　our team members and iGEM Tokyo_Tech 2013 veterans gave a presentation at a workshop called “MUSE TALK”. Managers, executives and technicians working in IT companies participated in this event to learn about things outside their specialty.

> Jump to Policy and Practices.

 
Fig. 7-5-2-1. Our presentation at MUSE TALK
 

After the presentation, many audiences were impressed with the technology of synthetic biology, and acknowledged it as a research which estimates future prospects.
 

 

 

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