Team:Glasgow/Parts
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<style type="text/css"> | <style type="text/css"> | ||
- | + | #biobricktable {border-collapse:collapse; | |
- | border:1px solid black;} | + | border:1px solid black; |
+ | cell-padding:5px; | ||
+ | text-align:center;} | ||
- | th {background-color:# | + | #biobricktable th {background-color:#000099; |
color:#FFFFFF; | color:#FFFFFF; | ||
border: 1px solid black; | border: 1px solid black; | ||
font-family:'Calibri'; | font-family:'Calibri'; | ||
} | } | ||
- | + | #biobricktable td {background-color:#FFFFFF; | |
- | td {background-color:# | + | |
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font-family:'Calibri';} | font-family:'Calibri';} | ||
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a:link {text-decoration:none;} | a:link {text-decoration:none;} | ||
a:visited {text-decoration:none; | a:visited {text-decoration:none; | ||
- | color:# | + | color:#0000CC;} |
a:hover {text-decoration:underline;} | a:hover {text-decoration:underline;} | ||
+ | |||
+ | .submits {color:#FF0000;} | ||
+ | |||
+ | #motlink:link,visited {text-decoration:underline; | ||
+ | font-weight:bold;} | ||
+ | |||
+ | #motlink:hover {text-decoration:underline; | ||
+ | font-weight:bold; | ||
+ | color:#0000CC;} | ||
+ | |||
+ | #collection {border-collapse:collapse; | ||
+ | table-layout:auto; | ||
+ | border:1px solid black; | ||
+ | font-family:"Calibri","Arial"; | ||
+ | |||
+ | text-align:center;} | ||
+ | |||
+ | #collection td {background-color:white; | ||
+ | border:1px solid black;} | ||
+ | |||
+ | #collection th {border:1px solid black;} | ||
+ | |||
+ | #collection tr {border:1px solid black;} | ||
+ | |||
+ | .topH {background-color:#0000CC; | ||
+ | color:#FFFFFF;} | ||
+ | |||
+ | #fliCH {background-color:#00FF00;} | ||
+ | |||
+ | #motAH {background-color:#FFFF00;} | ||
+ | |||
+ | #motBH {background-color:#FF0000;} | ||
+ | |||
+ | #combo {background-color:#FF9900;} | ||
+ | |||
+ | .collectionlink:link,visited {text-decoration:underline; | ||
+ | color:#000000;} | ||
+ | |||
+ | |||
+ | .collectionlink:hover {text-decoration:underline; | ||
+ | color:#FFFFFF;} | ||
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<body> | <body> | ||
<div id="maincontent"> | <div id="maincontent"> | ||
+ | <h2 class="pageheading">Biobricks</h2> | ||
<p> | <p> | ||
- | The following table lists the biobricks we created. Many of these have additional characterisation information, available on their respective Registry pages.<br> | + | The following table lists the biobricks we created. Many of these have additional characterisation information, available on their respective Registry pages. <br>We have also put together a <a id="motlink" href="#motility">Motility Parts Collection</a><br> |
Some important parts: | Some important parts: | ||
<ul> | <ul> | ||
- | <li> | + | <li>BBa_B0032 - Weak Ribosome Binding Site</li> |
- | <li> | + | <li>BBa_B0034 - Strong Ribosome Binding Site</li> |
- | <li></li> | + | <li>BBa_J61002 - Plasmid Vector</li> |
- | + | <li>BBa_J23xxx - Promoter</li> | |
</ul> | </ul> | ||
</p> | </p> | ||
+ | <p>Please note that all parts highlighted in <font color="red">RED</font> have been submitted and received by the registry. | ||
+ | </p> | ||
+ | |||
- | |||
- | <table> | + | <table id="biobricktable"> |
<tr> | <tr> | ||
<th>Biobrick Number</th> | <th>Biobrick Number</th> | ||
Line 53: | Line 98: | ||
</tr> | </tr> | ||
- | <tr> | + | <tr class="submits" > |
- | <td><a href="http://parts.igem.org/Part:BBa_K1463000" target="_window"> | + | <td><a href="http://parts.igem.org/Part:BBa_K1463000" target="_window">BBa_K1463000</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
- | <td>Switch Biobrick with RFP and GFP. | + | <td>Switch Biobrick with RFP and GFP. BBa_K1463520, BBa_K1463560, BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0034, BBa_E0040</td> |
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
+ | <td>1744</td> | ||
<td></td> | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K1463001" target="_window">BBa_K1463001</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>Switch Biobrick with just GFP. BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0034, BBa_E0040</td> | ||
+ | <td>iGEM14_Glasgow</td> | ||
+ | <td>1013</td> | ||
<td></td> | <td></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><a href="http://parts.igem.org/Part: | + | <td><a href="http://parts.igem.org/Part:BBa_K1463002" target="_window">BBa_K1463002</a></td> |
+ | <td>Composite</td> | ||
+ | <td>Switch Biobrick with just GFP weaker RBS. BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0032, BBa_E0040</td> | ||
+ | <td>iGEM14_Glasgow</td> | ||
+ | <td>1014</td> | ||
<td></td> | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K1463030" target="_window">BBa_K1463030</a></td> | ||
+ | <td>Basic</td> | ||
<td>Switch nucleotide spacer</td> | <td>Switch nucleotide spacer</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>11</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 73: | Line 136: | ||
<tr> | <tr> | ||
<td><a href="http://parts.igem.org/Part:BBa_K1463040" target="_window">BBa_K1463040</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1463040" target="_window">BBa_K1463040</a></td> | ||
- | <td></td> | + | <td>Basic</td> |
- | <td> | + | <td>φC31 attP site</td> |
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>54</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 82: | Line 145: | ||
<tr> | <tr> | ||
<td><a href="http://parts.igem.org/Part:BBa_K1463041" target="_window">BBa_K1463041</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1463041" target="_window">BBa_K1463041</a></td> | ||
- | <td></td> | + | <td>Basic</td> |
- | <td> | + | <td>φC31 attB site</td> |
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>55</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
- | <tr> | + | <tr class="submits"> |
<td><a href="http://parts.igem.org/Part:BBa_K1463050" target="_window">BBa_K1463050</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1463050" target="_window">BBa_K1463050</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>K1463040, K1463030, K1463501, B0010, K1463041</td> | <td>K1463040, K1463030, K1463501, B0010, K1463041</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>267</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 102: | Line 165: | ||
<td></td> | <td></td> | ||
<td>GvpA RBS</td> | <td>GvpA RBS</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>8</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
- | <tr> | + | <tr class="submits"> |
<td><a href="http://parts.igem.org/Part:BBa_K1463205" target="_window">BBa_K1463205</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1463205" target="_window">BBa_K1463205</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>B0034, K737016</td> | <td>B0034, K737016</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>237</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 120: | Line 183: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J61002, J23100, B0034, K737016</td> | <td>J61002, J23100, B0034, K737016</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>3226</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 129: | Line 192: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>K1463100, K737016</td> | <td>K1463100, K737016</td> | ||
+ | <td>iGEM14_Glasgow</td> | ||
+ | <td>233</td> | ||
<td></td> | <td></td> | ||
- | <td></td> | + | </tr> |
+ | |||
+ | |||
+ | <tr class="submits"> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K1463225" target="_window">BBa_K1463225</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>GvpC(K737017) and B0032</td> | ||
+ | <td>iGEM14_Glasgow</td> | ||
+ | <td>523</td> | ||
<td></td> | <td></td> | ||
</tr> | </tr> | ||
+ | |||
+ | |||
+ | |||
<tr> | <tr> | ||
Line 138: | Line 214: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>B0034, K737016, B0032, K737017</td> | <td>B0034, K737016, B0032, K737017</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>764</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 146: | Line 222: | ||
<tr> | <tr> | ||
- | <td><a href="http://parts.igem.org/Part: | + | <td><a href="http://parts.igem.org/Part:BBa_K1463345" target="_window">BBa_K1463345</a></td> |
<td>Composite</td> | <td>Composite</td> | ||
<td>J61002, J23100, B0034, K737016, B0032, K737017</td> | <td>J61002, J23100, B0034, K737016, B0032, K737017</td> | ||
+ | <td>iGEM14_Glasgow</td> | ||
+ | <td>3767</td> | ||
<td></td> | <td></td> | ||
- | <td></td> | + | </tr> |
+ | |||
+ | <tr class="submits"> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K1463350" target="_window">BBa_K1463350</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td>K1463100, K737016, B0032, K737017</td> | ||
+ | <td>iGEM14_Glasgow</td> | ||
+ | <td>764</td> | ||
<td></td> | <td></td> | ||
</tr> | </tr> | ||
+ | |||
+ | |||
+ | |||
<tr> | <tr> | ||
<td><a href="http://parts.igem.org/Part:BBa_K1463400" target="_window">BBa_K1463400</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1463400" target="_window">BBa_K1463400</a></td> | ||
<td></td> | <td></td> | ||
- | <td> | + | <td>φC31 Gp3</td> |
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>738</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 168: | Line 256: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>B0034, K1463400</td> | <td>B0034, K1463400</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>756</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 178: | Line 266: | ||
<td>Promoter</td> | <td>Promoter</td> | ||
<td>J23100 promoter in reverse</td> | <td>J23100 promoter in reverse</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>38</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 188: | Line 276: | ||
<td>Basic</td> | <td>Basic</td> | ||
<td>E1010 RFP in reverse</td> | <td>E1010 RFP in reverse</td> | ||
+ | <td>iGEM14_Glasgow</td> | ||
+ | <td>705</td> | ||
<td></td> | <td></td> | ||
- | <td></td> | + | </tr> |
+ | |||
+ | |||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K1463560" target="_window">BBa_K1463560</a></td> | ||
+ | <td>Basic</td> | ||
+ | <td> Strong reverse RBS</td> | ||
+ | <td>iGEM14_Glasgow</td> | ||
+ | <td>10</td> | ||
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 197: | Line 295: | ||
<td>Basic</td> | <td>Basic</td> | ||
<td>FliC</td> | <td>FliC</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>1497</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
- | <tr> | + | <tr class="submits"> |
<td><a href="http://parts.igem.org/Part:BBa_K1463601" target="_window">BBa_K1463601</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1463601" target="_window">BBa_K1463601</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>B0034, K1463600</td> | <td>B0034, K1463600</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>1515</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
- | <tr> | + | <tr class="submits"> |
<td><a href="http://parts.igem.org/Part:BBa_K1463602" target="_window">BBa_K1463602</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1463602" target="_window">BBa_K1463602</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J23116, B0032, B0034, K1463600</td> | <td>J23116, B0032, B0034, K1463600</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>1579</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
- | <tr> | + | |
+ | <tr class="submits"> | ||
<td><a href="http://parts.igem.org/Part:BBa_K1463603" target="_window">BBa_K1463603</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1463603" target="_window">BBa_K1463603</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
<td> J23106, B0032, B0034, K1463600</td> | <td> J23106, B0032, B0034, K1463600</td> | ||
+ | <td>iGEM14_Glasgow</td> | ||
+ | <td>1579</td> | ||
<td></td> | <td></td> | ||
- | <td></td> | + | </tr> |
+ | |||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K1463604" target="_window">BBa_K1463604</a></td> | ||
+ | <td>Composite</td> | ||
+ | <td> J23100, B0032, B0034, K1463600</td> | ||
+ | <td>iGEM14_Glasgow</td> | ||
+ | <td>1579</td> | ||
<td></td> | <td></td> | ||
</tr> | </tr> | ||
- | <tr> | + | <tr > |
<td><a href="http://parts.igem.org/Part:BBa_K1463700" target="_window">BBa_K1463700</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1463700" target="_window">BBa_K1463700</a></td> | ||
<td>Basic</td> | <td>Basic</td> | ||
<td>MotA</td> | <td>MotA</td> | ||
<td></td> | <td></td> | ||
- | <td></td> | + | <td>888</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
- | + | <tr class="submits"> | |
- | <tr> | + | |
<td><a href="http://parts.igem.org/Part:BBa_K1463701" target="_window">BBa_K1463701</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1463701" target="_window">BBa_K1463701</a></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>B0032, K1463700</td> | <td>B0032, K1463700</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>907</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 253: | Line 360: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J23100, B0032, K1463700</td> | <td>J23100, B0032, K1463700</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>950</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 263: | Line 370: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J23103, B0032, K1463700</td> | <td>J23103, B0032, K1463700</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>950</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 273: | Line 380: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J23106, B0032, K1463700</td> | <td>J23106, B0032, K1463700</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>950</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 283: | Line 390: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J23112, B0032, K1463700</td> | <td>J23112, B0032, K1463700</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>950</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 293: | Line 400: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J23116, B0032, K1463700</td> | <td>J23116, B0032, K1463700</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>950</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 303: | Line 410: | ||
<td>Basic</td> | <td>Basic</td> | ||
<td>MotB</td> | <td>MotB</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>927</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 313: | Line 420: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>B0032, K1463750</td> | <td>B0032, K1463750</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>946</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 323: | Line 430: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J23100, B0032, K1463750</td> | <td>J23100, B0032, K1463750</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>989</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 333: | Line 440: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J23103, B0032, K1463750</td> | <td>J23103, B0032, K1463750</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>989</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 342: | Line 449: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td> J23106, B0032, K1463750</td> | <td> J23106, B0032, K1463750</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>989</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 351: | Line 458: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J23112, B0032, K1463750</td> | <td>J23112, B0032, K1463750</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>989</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 360: | Line 467: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J23116, B0032, K1463750</td> | <td>J23116, B0032, K1463750</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>989</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 369: | Line 476: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J23103, B0032, K1463700, B0032, K1463750</td> | <td>J23103, B0032, K1463700, B0032, K1463750</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>1904</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 378: | Line 485: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J23112, B0032, K1463700, B0032, K1463750</td> | <td>J23112, B0032, K1463700, B0032, K1463750</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>1904</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 387: | Line 494: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J23106, B0032, K1463700, B0032, K1463750</td> | <td>J23106, B0032, K1463700, B0032, K1463750</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>1904</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 396: | Line 503: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J23116, B0032, K1463700, B0032, K1463750</td> | <td>J23116, B0032, K1463700, B0032, K1463750</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>1904</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 405: | Line 512: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J61002, J23103, E1010</td> | <td>J61002, J23103, E1010</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>3703</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 414: | Line 521: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J61002, J23106, E1010</td> | <td>J61002, J23106, E1010</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>3703</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 423: | Line 530: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td> J61002, J23112, E1010</td> | <td> J61002, J23112, E1010</td> | ||
- | <td></td> | + | <td>iGEM14_Glasgow</td> |
- | <td></td> | + | <td>3703</td> |
<td></td> | <td></td> | ||
</tr> | </tr> | ||
Line 432: | Line 539: | ||
<td>Composite</td> | <td>Composite</td> | ||
<td>J61002, J23116, E1010</td> | <td>J61002, J23116, E1010</td> | ||
+ | <td>iGEM14_Glasgow</td> | ||
+ | <td>3703</td> | ||
<td></td> | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | </table> | ||
+ | |||
+ | <a name="motility" ><h2 class="subheading">Motility Parts Collection</h2></a> | ||
+ | <p>The motility parts collection is the culmination of our work on bacterial motility genes - MotA, MotB and FliC. Our project plan required that, for the bacteria to float, these genes would need switched off. Therefore, work had to be done to: | ||
+ | </p> | ||
+ | <ul> | ||
+ | <li>Knock out the genes from the bacteria</li> | ||
+ | <li>Be able to recover the motility</li> | ||
+ | <li>Make the parts suitable for placement on one side of the recombinase switch</li> | ||
+ | </ul> | ||
+ | |||
+ | |||
+ | <table id="collection"> | ||
+ | <tr > | ||
+ | <th id="motBH" colspan="2"><a class="collectionlink" href="http://parts.igem.org/partsdb/uses.cgi?part=BBa_K1463750" target="_window">Contain MotB</a></th> | ||
+ | <th id="motAH" colspan="2"><a class="collectionlink" href="http://parts.igem.org/partsdb/uses.cgi?part=BBa_K1463700" target="_window">Contain MotA</a></th> | ||
+ | <th id="fliCH" colspan="2"><a class="collectionlink" href="http://parts.igem.org/partsdb/uses.cgi?part=BBa_K1463600" target="_window">Contain FliC</a></th> | ||
+ | </tr> | ||
+ | |||
+ | <tr class="topH"> | ||
+ | <th>Part Number</th> | ||
+ | <th>Description</th> | ||
+ | <th>Part Number</th> | ||
+ | <th>Description</th> | ||
+ | <th>Part Number</th> | ||
+ | <th>Description</th> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K1463750" target="_window">BBa_K1463750</a> </td> | ||
+ | <td>Basic MotB part</td> | ||
+ | <td>BBa_K1463700</td> | ||
+ | <td>Basic MotA</td> | ||
+ | <td>BBa_K1463600</td> | ||
+ | <td>The basic FliC part</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K1463751" target="_window">BBa_K1463751</a> </td> | ||
+ | <td>Composite B0032, K1463750</td> | ||
+ | <td>BBa_K1463701 </td> | ||
+ | <td>Composite B0032, K1463700</td> | ||
+ | <td>BBa_K1463601 </td> | ||
+ | <td>Composite B0034, K1463600</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K1463752" target="_window">BBa_K1463752</a> </td> | ||
+ | <td>Composite J23100, B0032, K1463750</td> | ||
+ | <td>BBa_K1463702</td> | ||
+ | <td> Composite J23100, B0032, K1463700</td> | ||
+ | <td>BBa_K1463602 </td> | ||
+ | <td>Composite J23116, B0032, B0034, K1463600</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K1463753" target="_window">BBa_K1463753</a> </td> | ||
+ | <td>Composite J23103, B0032, K1463750</td> | ||
+ | <td>BBa_K1463703</td> | ||
+ | <td>Composite J23103, B0032, K1463700</td> | ||
+ | <td>BBa_K1463603 </td> | ||
+ | <td>Composite J23106, B0032, B0034, K1463600</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K1463754" target="_window">BBa_K1463754</a> </td> | ||
+ | <td>Composite J23106, B0032, K1463750</td> | ||
+ | <td>BBa_K1463704 </td> | ||
+ | <td>Composite J23106, B0032, K1463700</td> | ||
+ | <td>BBa_K1463604 </td> | ||
+ | <td> Composite J23100, B0032, B0034, K1463600</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K1463755" target="_window">BBa_K1463755</a></td> | ||
+ | <td> Composite J23112, B0032, K1463750 </td> | ||
+ | <td>BBa_K1463705 </td> | ||
+ | <td>Composite J23112, B0032, K1463700</td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/Part:BBa_K1463756" target="_window">BBa_K1463756</a> </td> | ||
+ | <td>Composite J23116, B0032, K1463750</td> | ||
+ | <td>BBa_K1463706 </td> | ||
+ | <td>Composite J23116, B0032, K1463700</td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th id="combo" colspan="4">Contain MotA and MotB</th> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th class="topH" colspan="2">Part Number</th> | ||
+ | <th class="topH" colspan="2">Description</th> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td colspan="2"><a href="http://parts.igem.org/Part:BBa_K1463770" target="_window">BBa_K1463770</a> </td> | ||
+ | <td colspan="2">Composite J23103, B0032, K1463700, B0032, K1463750</td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td colspan="2"><a href="http://parts.igem.org/Part:BBa_K1463771" target="_window" >BBa_K1463771</a> </td> | ||
+ | <td colspan="2">Composite J23112, B0032, K1463700, B0032, K1463750</td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="2"><a href="http://parts.igem.org/Part:BBa_K1463772" target="_window" >BBa_K1463772</a> </td> | ||
+ | <td colspan="2">Composite J23106, B0032, K1463700, B0032, K1463750</td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td colspan="2"><a href="http://parts.igem.org/Part:BBa_K1463773" target="_window" >BBa_K1463773</a> </td> | ||
+ | <td colspan="2">Composite J23116, B0032, K1463700, B0032, K1463750</td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td colspan="2"><a href="http://parts.igem.org/Part:BBa_K1463760" target="_window">BBa_K1463760</a></td> | ||
+ | <td colspan="2">MotA and MotB with B0032 RBS</td> | ||
+ | <td></td> | ||
+ | <td></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td colspan="2"><a href="http://parts.igem.org/Part:BBa_K1463774" target="_window">BBa_K1463774</a></td> | ||
+ | <td colspan="2">MotA and MotB under J23100 Promoter with B0032 RBS.</td> | ||
<td></td> | <td></td> | ||
<td></td> | <td></td> | ||
Line 438: | Line 690: | ||
</table> | </table> | ||
+ | |||
+ | |||
+ | |||
+ | <a class="editlink" href="https://2014.igem.org/wiki/index.php?title=Team:Glasgow/Parts&action=edit">Edit</a> | ||
</div> | </div> |
Latest revision as of 02:48, 18 October 2014
Biobricks
The following table lists the biobricks we created. Many of these have additional characterisation information, available on their respective Registry pages.
We have also put together a Motility Parts Collection
Some important parts:
- BBa_B0032 - Weak Ribosome Binding Site
- BBa_B0034 - Strong Ribosome Binding Site
- BBa_J61002 - Plasmid Vector
- BBa_J23xxx - Promoter
Please note that all parts highlighted in RED have been submitted and received by the registry.
Biobrick Number | Type | Description | Designer | Length (BP) | Design Notes |
---|---|---|---|---|---|
BBa_K1463000 | Composite | Switch Biobrick with RFP and GFP. BBa_K1463520, BBa_K1463560, BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0034, BBa_E0040 | iGEM14_Glasgow | 1744 | |
BBa_K1463001 | Composite | Switch Biobrick with just GFP. BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0034, BBa_E0040 | iGEM14_Glasgow | 1013 | |
BBa_K1463002 | Composite | Switch Biobrick with just GFP weaker RBS. BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0032, BBa_E0040 | iGEM14_Glasgow | 1014 | |
BBa_K1463030 | Basic | Switch nucleotide spacer | iGEM14_Glasgow | 11 | |
BBa_K1463040 | Basic | φC31 attP site | iGEM14_Glasgow | 54 | |
BBa_K1463041 | Basic | φC31 attB site | iGEM14_Glasgow | 55 | |
BBa_K1463050 | Composite | K1463040, K1463030, K1463501, B0010, K1463041 | iGEM14_Glasgow | 267 | |
BBa_K1463100 | GvpA RBS | iGEM14_Glasgow | 8 | ||
BBa_K1463205 | Composite | B0034, K737016 | iGEM14_Glasgow | 237 | |
BBa_K1463207 | Composite | J61002, J23100, B0034, K737016 | iGEM14_Glasgow | 3226 | |
BBa_K1463210 | Composite | K1463100, K737016 | iGEM14_Glasgow | 233 | |
BBa_K1463225 | Composite | GvpC(K737017) and B0032 | iGEM14_Glasgow | 523 | |
BBa_K1463340 | Composite | B0034, K737016, B0032, K737017 | iGEM14_Glasgow | 764 | |
BBa_K1463345 | Composite | J61002, J23100, B0034, K737016, B0032, K737017 | iGEM14_Glasgow | 3767 | |
BBa_K1463350 | Composite | K1463100, K737016, B0032, K737017 | iGEM14_Glasgow | 764 | |
BBa_K1463400 | φC31 Gp3 | iGEM14_Glasgow | 738 | ||
BBa_K1463401 | Composite | B0034, K1463400 | iGEM14_Glasgow | 756 | |
BBa_K1463501 | Promoter | J23100 promoter in reverse | iGEM14_Glasgow | 38 | |
BBa_K1463520 | Basic | E1010 RFP in reverse | iGEM14_Glasgow | 705 | |
BBa_K1463560 | Basic | Strong reverse RBS | iGEM14_Glasgow | 10 | |
BBa_K1463600 | Basic | FliC | iGEM14_Glasgow | 1497 | |
BBa_K1463601 | Composite | B0034, K1463600 | iGEM14_Glasgow | 1515 | |
BBa_K1463602 | Composite | J23116, B0032, B0034, K1463600 | iGEM14_Glasgow | 1579 | |
BBa_K1463603 | Composite | J23106, B0032, B0034, K1463600 | iGEM14_Glasgow | 1579 | |
BBa_K1463604 | Composite | J23100, B0032, B0034, K1463600 | iGEM14_Glasgow | 1579 | |
BBa_K1463700 | Basic | MotA | 888 | ||
BBa_K1463701 | Composite | B0032, K1463700 | iGEM14_Glasgow | 907 | |
BBa_K1463702 | Composite | J23100, B0032, K1463700 | iGEM14_Glasgow | 950 | |
BBa_K1463703 | Composite | J23103, B0032, K1463700 | iGEM14_Glasgow | 950 | |
BBa_K1463704 | Composite | J23106, B0032, K1463700 | iGEM14_Glasgow | 950 | |
BBa_K1463705 | Composite | J23112, B0032, K1463700 | iGEM14_Glasgow | 950 | |
BBa_K1463706 | Composite | J23116, B0032, K1463700 | iGEM14_Glasgow | 950 | |
BBa_K1463750 | Basic | MotB | iGEM14_Glasgow | 927 | |
BBa_K1463751 | Composite | B0032, K1463750 | iGEM14_Glasgow | 946 | |
BBa_K1463752 | Composite | J23100, B0032, K1463750 | iGEM14_Glasgow | 989 | |
BBa_K1463753 | Composite | J23103, B0032, K1463750 | iGEM14_Glasgow | 989 | |
BBa_K1463754 | Composite | J23106, B0032, K1463750 | iGEM14_Glasgow | 989 | |
BBa_K1463755 | Composite | J23112, B0032, K1463750 | iGEM14_Glasgow | 989 | |
BBa_K1463756 | Composite | J23116, B0032, K1463750 | iGEM14_Glasgow | 989 | |
BBa_K1463770 | Composite | J23103, B0032, K1463700, B0032, K1463750 | iGEM14_Glasgow | 1904 | |
BBa_K1463771 | Composite | J23112, B0032, K1463700, B0032, K1463750 | iGEM14_Glasgow | 1904 | |
BBa_K1463772 | Composite | J23106, B0032, K1463700, B0032, K1463750 | iGEM14_Glasgow | 1904 | |
BBa_K1463773 | Composite | J23116, B0032, K1463700, B0032, K1463750 | iGEM14_Glasgow | 1904 | |
BBa_K1463805 | Composite | J61002, J23103, E1010 | iGEM14_Glasgow | 3703 | |
BBa_K1463806 | Composite | J61002, J23106, E1010 | iGEM14_Glasgow | 3703 | |
BBa_K1463807 | Composite | J61002, J23112, E1010 | iGEM14_Glasgow | 3703 | |
BBa_K1463808 | Composite | J61002, J23116, E1010 | iGEM14_Glasgow | 3703 |
Motility Parts Collection
The motility parts collection is the culmination of our work on bacterial motility genes - MotA, MotB and FliC. Our project plan required that, for the bacteria to float, these genes would need switched off. Therefore, work had to be done to:
- Knock out the genes from the bacteria
- Be able to recover the motility
- Make the parts suitable for placement on one side of the recombinase switch
Contain MotB | Contain MotA | Contain FliC | |||
---|---|---|---|---|---|
Part Number | Description | Part Number | Description | Part Number | Description |
BBa_K1463750 | Basic MotB part | BBa_K1463700 | Basic MotA | BBa_K1463600 | The basic FliC part |
BBa_K1463751 | Composite B0032, K1463750 | BBa_K1463701 | Composite B0032, K1463700 | BBa_K1463601 | Composite B0034, K1463600 |
BBa_K1463752 | Composite J23100, B0032, K1463750 | BBa_K1463702 | Composite J23100, B0032, K1463700 | BBa_K1463602 | Composite J23116, B0032, B0034, K1463600 |
BBa_K1463753 | Composite J23103, B0032, K1463750 | BBa_K1463703 | Composite J23103, B0032, K1463700 | BBa_K1463603 | Composite J23106, B0032, B0034, K1463600 |
BBa_K1463754 | Composite J23106, B0032, K1463750 | BBa_K1463704 | Composite J23106, B0032, K1463700 | BBa_K1463604 | Composite J23100, B0032, B0034, K1463600 |
BBa_K1463755 | Composite J23112, B0032, K1463750 | BBa_K1463705 | Composite J23112, B0032, K1463700 | ||
BBa_K1463756 | Composite J23116, B0032, K1463750 | BBa_K1463706 | Composite J23116, B0032, K1463700 | ||
Contain MotA and MotB | |||||
Part Number | Description | ||||
BBa_K1463770 | Composite J23103, B0032, K1463700, B0032, K1463750 | ||||
BBa_K1463771 | Composite J23112, B0032, K1463700, B0032, K1463750 | ||||
BBa_K1463772 | Composite J23106, B0032, K1463700, B0032, K1463750 | ||||
BBa_K1463773 | Composite J23116, B0032, K1463700, B0032, K1463750 | ||||
BBa_K1463760 | MotA and MotB with B0032 RBS | ||||
BBa_K1463774 | MotA and MotB under J23100 Promoter with B0032 RBS. |