Team:BostonU

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:BostonU&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<li><a href="https://2014.igem.org/Team:BostonU/Team">TEAM</a>
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<li><a href="">PROJECT</a>
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<li><a href="https://2014.igem.org/Team:BostonU/Chimera">Overview</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/Encoder">Priority Encoder</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/MoClo">MoClo Assembly Method</a></li>
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                        <li><a href="https://2014.igem.org/Team:BostonU/Backbones">Low Copy Backbones</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/ProjectTandemPromoters">Tandem Promoters</a></li>
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      <li><a href="https://2014.igem.org/Team:BostonU/FusionProteins">Fusion Proteins</a></li>
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      <li><a href="https://2014.igem.org/Team:BostonU/Repressors">Repressor Proteins</a></li>
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            <li><a href="https://2014.igem.org/Team:BostonU/Multiplexing">Multiplexing</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/Software">BioDesign Automation Tools</a></li>
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            <li><a href="https://2014.igem.org/Team:BostonU/Future">Future Work</a></li>
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<li><a href="">ACHIEVEMENTS</a>
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        <li><a href="https://2014.igem.org/Team:BostonU/Data">Data Collected</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/Parts">Parts Submitted</a></li>
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<a href="https://2014.igem.org/Team:BostonU"style="color:#000000">Home </a> </td>
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            <li><a href="https://2014.igem.org/Team:BostonU/Workflow">Chimera Workflow</a></li>
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            <li><a href="https://2014.igem.org/Team:BostonU/ChimeraExample">Chimera Example</a></li>
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            <li><a href="https://2014.igem.org/Team:BostonU/Medals">Medal Fulfillment</a></li>
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<a href="https://2014.igem.org/Team:BostonU/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=BostonU"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:BostonU/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:BostonU/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:BostonU/Modeling"style="color:#000000"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:BostonU/Notebook"style="color:#000000"> Notebook</a></td>
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<a href="https://2014.igem.org/Team:BostonU/Attributions"style="color:#000000"> Attributions </a></td>
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<ul>
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<li><a href="https://2014.igem.org/Team:BostonU">Home</a> </li>
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<li><a href="https://2014.igem.org/Team:BostonU/Team">Team</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=BostonU">Official Team Profile</a> </li>
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<li><a href="https://2014.igem.org/Team:BostonU/Project">Project</a> </li>
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<li><a href="https://2014.igem.org/Team:BostonU/Parts">Parts</a> </li>
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<li><a href="https://2014.igem.org/Team:BostonU/Modeling">Modeling</a> </li>
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<li><a href="https://2014.igem.org/Team:BostonU/Notebook">Notebook</a> </li>
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<li><a href="https://2014.igem.org/Team:BostonU/Safety">Safety</a> </li>
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<li><a href="https://2014.igem.org/Team:BostonU/Attributions">Attributions</a> </li>
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<li><a href="">NOTEBOOK</a>
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      <li><a href="https://2014.igem.org/Team:BostonU/Training">Training</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/Protocols">Protocols</a></li>
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                        <li><a href="https://2014.igem.org/Team:BostonU/BackbonesNotebook">Backbones</a></li>
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                        <li><a href="https://2014.igem.org/Team:BostonU/TandemPromoters">Tandem Promoters and Repressors</a></li>
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                        <li><a href="https://2014.igem.org/Team:BostonU/FusionProteinsNotebook">Fusion Proteins</a></li>
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<li><a href="">CONSIDERATIONS</a>
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<li><a href="https://2014.igem.org/Team:BostonU/Collaborations">Collaborations</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/Measurement">Measurement Track</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/Safety">Safety</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/Interlab">Interlab Study</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/NEGEM">NEGEM</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/HumanPractices">Policy and Practices</a></li>
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<li><a href="https://2014.igem.org/Team:BostonU/Acknowledgements">ACKNOWLEDGEMENTS</a>
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<header1>Chimera</header1><h3>an optimized characterization workflow for synthetic biology</h3>
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<h3>Abstract </h3>
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<strong>The Joy of Cloning: Chimera, a Recipe for Integrating Computational Tools with Experimental Protocols </strong><br>
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If BU can clone it, so can you! With a pinch of wet lab work and a dash of computational tools, we have developed a new recipe called <a href="https://2014.igem.org/Team:BostonU/Workflow">Chimera</a> that will help fellow synthetic biology cloners in the creation of their genetic devices! Chimera utilizes <a href="https://2014.igem.org/Team:BostonU/Software">bio-design automation software tools</a> with experimental protocols and builds upon a thoroughly characterized library of <a href="https://2014.igem.org/Team:BostonU/MoClo">MoClo</a> parts. This recipe, or workflow, integrates software tools to reduce human error and to structure the way device designs are chosen, assembled, and tested. To demonstrate that this workflow can be used by any level of cloner (beginner, intermediate, and advanced), we will highlight how we used Chimera to create:<br>
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<p class="tab"><li> individual genetic parts (namely <a href="https://2014.igem.org/Team:BostonU/ProjectTandemPromoters">tandem promoters</a>, <a href="https://2014.igem.org/Team:BostonU/FusionProteins">fusion proteins</a>, and <a href="https://2014.igem.org/Team:BostonU/Backbones">different backbones</a>), </p>
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<p class="tab"><li> transcriptional units assembled from individual parts (with both new and previously made MoClo parts), and </p>
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<p class="tab"><li> a complex genetic device (our goal is a <a href="https://2014.igem.org/Team:BostonU/Encoder">priority encoder</a>).</p>
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<br><br><strong><a href="https://2014.igem.org/Team:BostonU/Chimera">Click here to explore our project!</a></strong></td></tr></table>
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<div class="sponsors" align="center"> <br><br><header1>Our Sponsors</header1><br><br><img src="https://static.igem.org/mediawiki/2014/c/c5/Sponsors_bu14.png" width="983" height="149"> </div>
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<p>We are currently working on providing teams with some easy to use design templates.
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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<li>Have lots of fun! </li>
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Latest revision as of 03:24, 18 October 2014



Chimera

an optimized characterization workflow for synthetic biology


Abstract

The Joy of Cloning: Chimera, a Recipe for Integrating Computational Tools with Experimental Protocols
If BU can clone it, so can you! With a pinch of wet lab work and a dash of computational tools, we have developed a new recipe called Chimera that will help fellow synthetic biology cloners in the creation of their genetic devices! Chimera utilizes bio-design automation software tools with experimental protocols and builds upon a thoroughly characterized library of MoClo parts. This recipe, or workflow, integrates software tools to reduce human error and to structure the way device designs are chosen, assembled, and tested. To demonstrate that this workflow can be used by any level of cloner (beginner, intermediate, and advanced), we will highlight how we used Chimera to create:

  • individual genetic parts (namely tandem promoters, fusion proteins, and different backbones),

  • transcriptional units assembled from individual parts (with both new and previously made MoClo parts), and

  • a complex genetic device (our goal is a priority encoder).



    Click here to explore our project!
    •


    Our Sponsors

    Retrieved from "http://2014.igem.org/Team:BostonU"