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| + | {{Team:Oxford/templates/header}} |
| + | |
| <html> | | <html> |
- | <head>
| |
- | <style type="text/css">
| |
| | | |
- | @import url(http://fonts.googleapis.com/css?family=PT+Serif|Open+Sans:300italic,400,300);
| |
| | | |
- | html, body {
| + | <body> |
- | max-width: 100%;
| + | <div class="outer" style="overflow-y: scroll; overflow-x: hidden;"> |
- | overflow-x: hidden;
| + | |
- | }
| + | |
| | | |
- | body {
| + | <div id="stuff" style="float:left;position:absolute;margin-left:200px;margin-right:100px; margin-top:50px;min-width:645px;"> |
- | background-color: #EBEBEB;
| + | <div id="showwetlab"> |
- | margin: 0px;
| + | <div id="showmodelling"> |
- | padding: 0;
| + | <img src="https://static.igem.org/mediawiki/2014/0/0f/Real_Bioremediation.jpg" style="position:absolute; width:100%;z-index:-1; border-radius:15px;margin-top:-10px;"/> |
- | border: 0;
| + | |
- | vertical-align: baseline;
| + | |
- | font-family: 'Open Sans', 'helvetica', sans-serif;
| + | |
- | font-size: 10px;
| + | |
- | font-weight: 300;
| + | |
- | }
| + | |
| | | |
- | #content {
| + | <div style="background-color:#D9D9D9; opacity:0.7; z-index:5; Height:75px; width:100%;font-size:65px;font-family:Helvetica;padding-top:5px; font-weight: 450;margin-top:10px;"> |
- | border:none;
| + | <div style="background-color:white; opacity:0.9; Height:75px; width:100%;margin-top:5px:margin-bottom:5px;font-size:65px;font-family:Helvetica;padding-top:5px; color:#00000; font-weight: 450;"><br><center><font style="opacity:0.7">What are they?</font></center></div> |
- | width:100%;
| + | </div> |
- | margin:0;
| + | <br> |
- | padding:0;
| + | |
- | background:none;
| + | |
- | }
| + | |
| | | |
- | #catlinks {
| + | <div style="border-bottom-left-radius:10px;border-bottom-right-radius:10px; padding-left:10px;padding-right:10px;min-width:300px;"> |
- | display:none;
| + | |
- | }
| + | |
| | | |
- | #contentSub {
| |
- | display:none;
| |
- | }
| |
| | | |
- | #menubar {
| + | <img src="https://static.igem.org/mediawiki/2014/d/d5/Realisation_bioremediation.png" style="width:35%;margin-left:33%;margin-top:-50px;"> |
- | width: auto;
| + | |
- | margin-top:0.5%;
| + | |
- | }
| + | |
| | | |
- | .left-menu:hover {
| |
- | background-color: transparent;
| |
- | }
| |
- | #menubar li a {
| |
- | background-color: transparent;
| |
- | }
| |
- | #menubar:hover {
| |
- | color: gray;
| |
- | }
| |
- | #menubar li a {
| |
- | color: #B5B5B5;
| |
- | }
| |
- | #menubar:hover li a {
| |
- | color: gray;
| |
- | }
| |
| | | |
- | #menubar.left-menu.noprint ul li:last-child {
| |
- | display:none;
| |
- | }
| |
| | | |
- | #search-controls {
| + | </div> |
- | display:none;
| + | <div style="background-color:white; border-bottom-left-radius:10px;border-radius:10px; padding-left:10px;padding-right:10px;min-width:300px;margin-top:-50px;"> |
- | }
| + | <a href="https://static.igem.org/mediawiki/2014/3/3d/OxigemLAB_BOOK.pdf" target="_blank"><img src="https://static.igem.org/mediawiki/2014/5/50/OxigemLabbook.png" style="position:absolute;width:6%;margin-left:84%;margin-top:-13%;z-index:10;"></a> |
| + | <a href="https://static.igem.org/mediawiki/2014/1/16/Oxigem_LAB_PROTOCOLS.pdf" target="_blank"><img src="https://static.igem.org/mediawiki/2014/a/a4/OxigemProtocols.png" style="position:absolute;width:6%;margin-left:91%;margin-top:-13%;z-index:10;"></a> |
| | | |
- | #top-section {
| + | <div style="position:absolute;background-color:rgba(255,255,255,0.6);border-radius:15px; z-index:5;margin-top:-18.2%; Height:70px; width:20%;font-size:65px;font-family:Helvetica; font-weight: 450;padding-left:10px;padding-right:10px;padding-top:3px;min-width:170px;margin-bottom:3px;"> |
- | height:0px;
| + | |
- | width:100%;
| + | <div style="width:100%;"><font style="font-size:15px;font-weight:500;">Show all:</font></div> |
- | border:none;
| + | |
- | }
| + | |
| | | |
- | #footer-box {
| + | <a href="#showmodelling"><div class="orange_news_block1 showmodelling" style="background: #F9A7B0;border-radius:15px;color:black;float:left;height:40%;width:40%;margin-left:6%;padding-top:10px;"><center> |
- | border:none;
| + | <h1white><font style="font-size:15px;font-weight:500;">Modelling</font></h1white></center> |
- | display:none;
| + | </div></a> |
- | width:100%;
| + | |
- | }
| + | |
| | | |
- | #p-logo {
| + | <a href="#showwetlab"><div class="orange_news_block1 showwetlab" style="background: #ADD8E6;border-radius:15px;color:black;float:left;height:40%;width:40%;margin-left:3%;padding-top:10px;"><center> |
- | display:none
| + | <h1white><font style="font-size:15px;font-weight:500;">Wetlab</font></h1white></center> |
- | }
| + | </div></a> |
- | | + | <br><br><br><br><br> |
- | .firstHeading {
| + | |
- | display:none;
| + | |
- | }
| + | |
- | | + | |
- | #bodyContent p{
| + | |
- | display:none;
| + | |
- | }
| + | |
- | | + | |
- | html, div, span, applet, object, iframe,
| + | |
- | h1, h2, h3, h4, h5, h6, p, blockquote, pre,
| + | |
- | a, abbr, acronym, address, big, cite, code,
| + | |
- | del, dfn, em, img, ins, kbd, q, s, samp,
| + | |
- | small, strike, strong, sub, sup, tt, var,
| + | |
- | b, u, i, center,
| + | |
- | dl, dt, dd, ol, ul, li,
| + | |
- | fieldset, form, label, legend,
| + | |
- | table, caption, tbody, tfoot, thead, tr, th, td,
| + | |
- | article, aside, canvas, details, embed,
| + | |
- | figure, figcaption, footer, header, hgroup,
| + | |
- | menu, nav, output, ruby, section, summary,
| + | |
- | time, mark, audio, video {
| + | |
- | margin: 0;
| + | |
- | padding: 0;
| + | |
- | border: 0;
| + | |
- | font-size: 100%;
| + | |
- | font: inherit;
| + | |
- | vertical-align: baseline;
| + | |
- | }
| + | |
- | | + | |
- | #top-section {
| + | |
- | width: 965px;
| + | |
- | height: 0;
| + | |
- | margin: 0 auto;
| + | |
- | padding: 0;
| + | |
- | border: none;}
| + | |
- | | + | |
- | .left-menu:hover {
| + | |
- | background-color: transparent;}
| + | |
- | | + | |
- | #menubar li a {
| + | |
- | background-color: transparent;}
| + | |
- | | + | |
- | .outer {
| + | |
- | position:fixed;
| + | |
- | width:100%;
| + | |
- | height:100%;
| + | |
- | }
| + | |
- | | + | |
- | img.supercoil {
| + | |
- | width:65%;
| + | |
- | display: block;
| + | |
- | margin-left: auto;
| + | |
- | margin-right: auto }
| + | |
- | | + | |
- | .Heading {
| + | |
- | position:absolute;
| + | |
- | display: block;
| + | |
- | }
| + | |
- | | + | |
- | .heading {
| + | |
- | -webkit-transition: margin-left 0.3s, -webkit-transform 0.3s;
| + | |
- | transition: margin-left 0.3s;
| + | |
- | }
| + | |
- | | + | |
- | .heading:hover {
| + | |
- | margin-left:30px;
| + | |
- | }
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | .pink_news_block {
| + | |
- |
| + | |
- | background: #DEA5A4;
| + | |
- | border-radius:15px; margin-top:2%; padding-left:2%;padding-right:2%;padding-top:3%;padding-bottom:3%;
| + | |
- | width:96%;
| + | |
- | }
| + | |
- | | + | |
- | .blue_news_block {
| + | |
- |
| + | |
- | background: #ADD8E6;
| + | |
- | border-radius:30px; margin-top:2%; padding-left:2%;padding-right:2%;padding-top:2%;padding-bottom:2%;
| + | |
- | width:96%; | + | |
- | }
| + | |
- | | + | |
- | .orange_news_block1 {
| + | |
- |
| + | |
- | background: #F9A7B0;
| + | |
- | border-radius:30px; margin-top:2%; padding-left:2%;padding-right:2%;padding-top:2%;padding-bottom:2%;
| + | |
- | width:32%;
| + | |
- | }
| + | |
- | | + | |
- | .orange_news_block2 {
| + | |
- |
| + | |
- | background: #F9A7B0;
| + | |
- | border-radius:30px; margin-top:2%; padding-left:2%;padding-right:2%;padding-top:2%;padding-bottom:2%;
| + | |
- | width:96%;
| + | |
- | }
| + | |
- | | + | |
- | .yellow_news_block {
| + | |
- |
| + | |
- | background: #FFFF06;
| + | |
- | border-radius:30px; margin-top:2%; padding-left:2%;padding-right:2%;padding-top:2%;padding-bottom:2%;
| + | |
- | width:27%;
| + | |
- | }
| + | |
- | .white_news_block {
| + | |
- |
| + | |
- | background: #fff;
| + | |
- | border-radius:15px; margin-top:2%; margin-left: 0%; padding-left:2%;padding-right:2%;padding-top:2%;padding-bottom:2%;
| + | |
- | width:96%;
| + | |
- | }
| + | |
- | | + | |
- | h1 {
| + | |
- | font-size: 120%;
| + | |
- | font-weight: 400;
| + | |
- | font-family:Helvetica;
| + | |
- | margin-bottom: 2%;
| + | |
- |
| + | |
- | }
| + | |
- | h1white { | + | |
- | font-size: 230%;
| + | |
- | font-weight: 400;
| + | |
- | font-family:Helvetica;
| + | |
- | color:#000000;
| + | |
- | line-height:110%;
| + | |
- | }
| + | |
- | | + | |
- | h1black {
| + | |
- | font-size: 230%;
| + | |
- | font-weight: 400;
| + | |
- | font-family:Helvetica;
| + | |
- | color:#030B79;
| + | |
- | line-height:110%;
| + | |
- | }
| + | |
- | | + | |
- | </style>
| + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | | + | |
- | #list li {
| + | |
- | list-style-image: url("https://static.igem.org/mediawiki/2014/6/6f/OxigemTick.png"); }
| + | |
- | }
| + | |
- | </style>
| + | |
- | <script type="text/javascript">
| + | |
- | | + | |
- | function iconleft(x) {
| + | |
- | x.style.left="6%";
| + | |
- | }
| + | |
- | function iconnormal(x) {
| + | |
- | x.style.left="9%";
| + | |
- | }
| + | |
- | $(document).ready(function(){
| + | |
- | $("#arrowr").click(function(){
| + | |
- | $("#grey").animate({right:'-175px'}, "fast");
| + | |
- | });
| + | |
- | $("#arrowl").click(function(){
| + | |
- | $("#grey").animate({right:'-120px'}, "fast");
| + | |
- | });
| + | |
- | $("#modelling").hover(function(){
| + | |
- | $("#modelling").animate({opacity:'0.95'}, "fast");
| + | |
- | $("#modelling1").css({display:'inline'});
| + | |
- | });
| + | |
- | $("#modelling").mouseout(function(){
| + | |
- | $("#modelling").animate({opacity:'0.75'}, "fast");
| + | |
- | $("#modelling1").css({display:'none'});
| + | |
- | });
| + | |
- | $("#achievements").hover(function(){
| + | |
- | $("#achievements").animate({opacity:'0.95'}, "fast");
| + | |
- | $("#achievements1").css({display:'inline'});
| + | |
- | });
| + | |
- | $("#achievements").mouseout(function(){
| + | |
- | $("#achievements").animate({opacity:'0.75'}, "fast");
| + | |
- | $("#achievements1").css({display:'none'});
| + | |
- | });
| + | |
- | $("#igemcomm").hover(function(){
| + | |
- | $("#igemcomm").animate({opacity:'0.95'}, "fast");
| + | |
- | $("#igemcomm1").css({display:'inline'});
| + | |
- | });
| + | |
- | $("#igemcomm").mouseout(function(){
| + | |
- | $("#igemcomm").animate({opacity:'0.75'}, "fast");
| + | |
- | $("#igemcomm1").css({display:'none'});
| + | |
- | });
| + | |
- | $("#safety").hover(function(){
| + | |
- | $("#safety").animate({opacity:'0.95'}, "fast");
| + | |
- | $("#safety1").css({display:'inline'});
| + | |
- | });
| + | |
- | $("#safety").mouseout(function(){
| + | |
- | $("#safety").animate({opacity:'0.75'}, "fast");
| + | |
- | $("#safety1").css({display:'none'});
| + | |
- | });
| + | |
- | $("#team").hover(function(){
| + | |
- | $("#team").animate({opacity:'0.95'}, "fast");
| + | |
- | $("#team1").css({display:'inline'});
| + | |
- | });
| + | |
- | $("#team").mouseout(function(){
| + | |
- | $("#team").animate({opacity:'0.75'}, "fast");
| + | |
- | $("#team1").css({display:'none'});
| + | |
- | });
| + | |
- | $("#dcmation").hover(function(){
| + | |
- | $("#dcmation").animate({opacity:'0.95'}, "fast");
| + | |
- | $("#dcmation1").css({display:'inline'});
| + | |
- | });
| + | |
- | $("#dcmation").mouseout(function(){
| + | |
- | $("#dcmation").animate({opacity:'0.75'}, "fast");
| + | |
- | $("#dcmation1").css({display:'none'});
| + | |
- | });
| + | |
- | $("#interlab").hover(function(){
| + | |
- | $("#interlab").animate({opacity:'0.95'}, "fast");
| + | |
- | $("#interlab1").css({display:'inline'});
| + | |
- | });
| + | |
- | $("#interlab").mouseout(function(){
| + | |
- | $("#interlab").animate({opacity:'0.75'}, "fast");
| + | |
- | $("#interlab1").css({display:'none'});
| + | |
- | });
| + | |
- | $("#policy").hover(function(){
| + | |
- | $("#policy").animate({opacity:'0.95'}, "fast");
| + | |
- | $("#policy1").css({display:'inline'});
| + | |
- | });
| + | |
- | $("#policy").mouseout(function(){
| + | |
- | $("#policy").animate({opacity:'0.75'}, "fast");
| + | |
- | $("#policy1").css({display:'none'});
| + | |
- | });
| + | |
- | });
| + | |
- | | + | |
- | | + | |
- | jQuery(document).ready(function(){
| + | |
- | jQuery('#tb1').live('click', function(event) {
| + | |
- | jQuery('#b1').toggle('show');
| + | |
- | });
| + | |
- | jQuery('#tb2').live('click', function(event) {
| + | |
- | jQuery('#b2').toggle('show');
| + | |
- | });
| + | |
- | jQuery('#tb3').live('click', function(event) {
| + | |
- | jQuery('#b3').toggle('show');
| + | |
- | });
| + | |
- | jQuery('#tb4').live('click', function(event) {
| + | |
- | jQuery('#b4').toggle('show');
| + | |
- | });
| + | |
- | jQuery('#tb5').live('click', function(event) {
| + | |
- | jQuery('#b5').toggle('show');
| + | |
- | });
| + | |
- | jQuery('#ts1').live('click', function(event) {
| + | |
- | jQuery('#s1').toggle('show');
| + | |
- | });
| + | |
- | jQuery('#ts2').live('click', function(event) {
| + | |
- | jQuery('#s2').toggle('show');
| + | |
- | });
| + | |
- | jQuery('#ts3').live('click', function(event) {
| + | |
- | jQuery('#s3').toggle('show');
| + | |
- | });
| + | |
- | jQuery('#ts4').live('click', function(event) {
| + | |
- | jQuery('#s4').toggle('show');
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- | });
| + | |
- | jQuery('#tg1').live('click', function(event) {
| + | |
- | jQuery('#g1').toggle('show');
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- | });
| + | |
- | jQuery('#tg2').live('click', function(event) {
| + | |
- | jQuery('#g2').toggle('show');
| + | |
- | });
| + | |
- | jQuery('#tg3').live('click', function(event) {
| + | |
- | jQuery('#g3').toggle('show');
| + | |
- | });
| + | |
- | });
| + | |
- | | + | |
- | | + | |
- | function startup(){
| + | |
- | $("#sidebarblue").animate({marginLeft:'+=250px'}, 1000);
| + | |
- | $("#sidebarlogo").animate({marginLeft:'+=250px'}, 1000);
| + | |
- | }
| + | |
- | function startup1(){
| + | |
- | $(".1").animate({marginLeft:'+=250px'}, 1000);
| + | |
- | }
| + | |
- | function startup2(){
| + | |
- | $(".2").animate({marginLeft:'+=250px'}, 1000);
| + | |
- | }
| + | |
- | function startup3(){
| + | |
- | $(".3").animate({marginLeft:'+=250px'}, 1000);
| + | |
- | }
| + | |
- | function startup4(){
| + | |
- | $(".4").animate({marginLeft:'+=250px'}, 1000);
| + | |
- | }
| + | |
- | | + | |
- | | + | |
- | | + | |
- | $(document).ready(function(){
| + | |
- | setTimeout(function() {
| + | |
- | startup();
| + | |
- | }, 1500);
| + | |
- | setTimeout(function() {
| + | |
- | startup1();
| + | |
- | }, 1520);
| + | |
- | setTimeout(function() {
| + | |
- | startup2();
| + | |
- | }, 1560);
| + | |
- | setTimeout(function() {
| + | |
- | startup3();
| + | |
- | }, 1640);
| + | |
- | setTimeout(function() {
| + | |
- | startup4();
| + | |
- | }, 1750);
| + | |
- | });
| + | |
- | | + | |
- | </script>
| + | |
- | | + | |
- | <title></title>
| + | |
- | </head> | + | |
- | | + | |
- | <body>
| + | |
- | <div class="outer" style="overflow-y: scroll; overflow-x: hidden;">
| + | |
- | | + | |
- | | + | |
- | | + | |
- | <div id="stuff" style="float:left;position:absolute;margin-left:200px;margin-right:100px; margin-top:50px;"> | + | |
- | | + | |
- | <img src="https://static.igem.org/mediawiki/2014/0/08/Oxigemradcamcrop.jpg" style="position:absolute; width:100%;min-width:100%;z-index:-1; border-radius:15px;"/> | + | |
- | | + | |
- | | + | |
- | <div style="background-color:#D9D9D9; opacity:0.7; z-index:5; margin-right:auto;margin-left:auto; Height:75px; width:90%;min-width:300px;font-size:65px;font-family:Helvetica;padding-top:5px; font-weight: 450;"> | + | |
- | <div style="background-color:white; opacity:0.7; Height:75px; width:100%;margin-top:5px:margin-bottom:5px;min-width:300px;font-size:65px;font-family:Helvetica;padding-top:5px; color:#596C8A; font-weight: 450;"><br>What are they?</div> | + | |
| </div> | | </div> |
| + | <br> |
| + | <h1>Introduction: what are microcompartments?</h1> |
| + | In order to produce an efficient bioremediation system, we had to think outside the box and not only improve the expression and function proteins we were working with, but optimise the bacteria themselves for the degradation of toxic compounds. We found that microcompartments, which are explained on this page, are ideally suited for our purposes. |
| <br><br> | | <br><br> |
| + | </div> |
| + | |
| | | |
| | | |
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| | | |
| | | |
- | <div class="white_news_block">
| |
- | <h1>Introduction: what are microcompartments?</h1>
| |
- | In order to produce an efficient bioremediation system, we had to think outside the box and not only improve the expression and function proteins we were working with, but optimise the bacteria themselves for the degradation of toxic compounds. We found that microcompartments, which are explained on this page, are ideally suited for our purposes.
| |
- | </div>
| |
| | | |
| | | |
| | | |
- | <head>
| |
- | <style type="text/css">
| |
- | .row { vertical-align: top; height:auto !important; }
| |
- | .list {display:none; }
| |
- | .show {display: none; }
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| <div class="row"> | | <div class="row"> |
- | <a href="#hide1" class="hide" id="hide1"><div class="blue_news_block"> | + | <a href="#show1" class="show wetlab-row" id="show1"><div class="wetlab"> |
- | <h1black>Structure</h1black> | + | <h1white>Structure</h1white> |
| <img src="https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png" style="float:right;position:relative; width:2%;" /> | | <img src="https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png" style="float:right;position:relative; width:2%;" /> |
| </div></a> | | </div></a> |
| | | |
- | <a href="#show1" class="show" id="show1"><div class="blue_news_block"> | + | <a href="#hide1" class="hide" id="hide1"><div class="wetlab"> |
- | <h1black>Structure</h1black> | + | <h1white>Structure</h1white></div></a> |
- | </div></a> | + | |
| <div class="list"> | | <div class="list"> |
- | <div class="white_news_block"> | + | <div class="white_news_block2"> |
| | | |
| <img src="https://static.igem.org/mediawiki/2014/1/1e/Oxford_microcomp1.png" style="float:right;position:relative; width:40%;" /> | | <img src="https://static.igem.org/mediawiki/2014/1/1e/Oxford_microcomp1.png" style="float:right;position:relative; width:40%;" /> |
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| </div> | | </div> |
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| <div class="row"> | | <div class="row"> |
- | <a href="#hide2" class="hide" id="hide2"><div class="orange_news_block2"> | + | <a href="#show2" class="show modelling-row" id="show2"><div class="modelling"> |
| <h1white>Predicting the microcompartment structure</h1white> | | <h1white>Predicting the microcompartment structure</h1white> |
| <img src="https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png" style="float:right;position:relative; width:2%;" /> | | <img src="https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png" style="float:right;position:relative; width:2%;" /> |
| </div></a> | | </div></a> |
| | | |
- | <a href="#show2" class="show" id="show2"><div class="orange_news_block2"> | + | <a href="#hide2" class="hide" id="hide2"><div class="modelling"> |
- | <h1white>Predicting the microcompartment structure</h1white> | + | <h1white>Predicting the microcompartment structure</h1white></div></a> |
- | </div></a> | + | |
| <div class="list"> | | <div class="list"> |
- | <div class="white_news_block"> | + | <div class="white_news_block2"> |
| The pdu-encoded microcompartments which we are introducing into our bacteria are very similar in shape and structure to carboxysomes, so we used their structure as a starting point for predicting the microcompartment structure. Thus, our initial carboxysome-based structure was a perfect icosahedron with diameter 120nm as established from literature. Following this, we further developed the model by noting that many of the images taken of pdu microcompartments suggest slight and random deviations from an icosahedron. | | The pdu-encoded microcompartments which we are introducing into our bacteria are very similar in shape and structure to carboxysomes, so we used their structure as a starting point for predicting the microcompartment structure. Thus, our initial carboxysome-based structure was a perfect icosahedron with diameter 120nm as established from literature. Following this, we further developed the model by noting that many of the images taken of pdu microcompartments suggest slight and random deviations from an icosahedron. |
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| <div class="row"> | | <div class="row"> |
- | <a href="#hide3" class="hide" id="hide3"><div class="blue_news_block"> | + | <a href="#show3" class="show wetlab-row" id="show3"><div class="wetlab"> |
- | <h1black>Abundance</h1black> | + | <h1white>Abundance</h1white> |
| <img src="https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png" style="float:right;position:relative; width:2%;" /> | | <img src="https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png" style="float:right;position:relative; width:2%;" /> |
| </div></a> | | </div></a> |
| | | |
- | <a href="#show3" class="show" id="show3"><div class="blue_news_block"> | + | <a href="#hide3" class="hide" id="hide3"><div class="wetlab"> |
- | <h1black>Abundance</h1black> | + | <h1white>Abundance</h1white></div></a> |
- | </div></a> | + | |
| <div class="list"> | | <div class="list"> |
- | <div class="white_news_block">
| + | <div class="white_news_block2"> |
| The number of microcompartments in a given cell can vary broadly, from a few microsomes to numbers that occupy the vast amount of the cytosol. Because of their size and the number of protein components, microcompartments are an extensive strain on the metabolism of cells. We have placed the E. coli ABTUNJK codon under control of the T7 promoter, while that for P. putida is placed under control of the T3 promoter. | | The number of microcompartments in a given cell can vary broadly, from a few microsomes to numbers that occupy the vast amount of the cytosol. Because of their size and the number of protein components, microcompartments are an extensive strain on the metabolism of cells. We have placed the E. coli ABTUNJK codon under control of the T7 promoter, while that for P. putida is placed under control of the T3 promoter. |
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| <div class="row"> | | <div class="row"> |
- | <a href="#hide4" class="hide" id="hide4"><div class="orange_news_block2"> | + | <a href="#show4" class="show modelling-row" id="show4"><div class="modelling"> |
| <h1white>Modelling the number of enzymes in a microcompartment</h1white> | | <h1white>Modelling the number of enzymes in a microcompartment</h1white> |
| <img src="https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png" style="float:right;position:relative; width:2%;" /> | | <img src="https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png" style="float:right;position:relative; width:2%;" /> |
| </div></a> | | </div></a> |
| | | |
- | <a href="#show4" class="show" id="show4"><div class="orange_news_block2"> | + | <a href="#hide4" class="hide" id="hide4"><div class="modelling"> |
- | <h1white>Modelling the number of enzymes in a microcompartment</h1white> | + | <h1white>Modelling the number of enzymes in a microcompartment</h1white></div></a> |
- | </div></a> | + | |
| <div class="list"> | | <div class="list"> |
- | <div class="white_news_block"> | + | <div class="white_news_block2"> |
| The aim of this model was to predict a theoretical maximum number of enzyme molecules that can be packed into a single microcompartment. To get a first estimate, without taking into consideration whether this volume of protein would interrupt the biological processes in the cell, we approached the problem volumetrically. | | The aim of this model was to predict a theoretical maximum number of enzyme molecules that can be packed into a single microcompartment. To get a first estimate, without taking into consideration whether this volume of protein would interrupt the biological processes in the cell, we approached the problem volumetrically. |
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- | <img src="https://static.igem.org/mediawiki/2014/8/8f/Oxford_Leroy_table1.png" style="float:left;position:relative; width:50%;margin-left:25%;margin-right:25%;" /> | + | <img src="https://static.igem.org/mediawiki/2014/8/8f/Oxford_Leroy_table1.png" style="float:left;position:relative; width:50%;margin-left:25%;margin-right:25%;margin-bottom:10px;" /> |
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- | <br><br><br><br>
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| We should note that these numbers are a first approximation and do not take into consideration the strain placed on the cell involved in expressing this amount of protein. This model is based entirely on volumetric and geometric laws rather than biological ones and should be considered a first assumption and theoretical maximum given optimum conditions. | | We should note that these numbers are a first approximation and do not take into consideration the strain placed on the cell involved in expressing this amount of protein. This model is based entirely on volumetric and geometric laws rather than biological ones and should be considered a first assumption and theoretical maximum given optimum conditions. |
| </div> | | </div> |
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- | <div class="white_news_block"> | + | <div class="row"> |
| + | <a href="#show13" class="show wetlab-row" id="show13"><div class="wetlab"> |
| + | <h1white>Targeting into the microcompartment</h1white> |
| + | <img src="https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png" style="float:right;position:relative; width:2%;" /> |
| + | </div></a> |
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| + | <a href="#hide13" class="hide" id="hide12"><div class="wetlab"> |
| + | <h1white>Targeting into the microcompartment</h1white></div></a> |
| + | <div class="list"> |
| + | <div class="white_news_block2"> |
| + | Proteins can be inserted into the microcompartment when they have an N-terminal targeting sequence. |
| + | In order to show that this targeting mechanism works for our constructs, we have constructed a plasmid with sfGFP fused to the N-terminal tag. When transforming this vector into E.coli along with the pUNI-ABTUNJK vector, we observed high-density fluorescence spots in those cells that expressed both the microcompartment and microcompartment-tagged sfGFP. This is shown in the image on the left, whereas the image on the right shows a control sample without the pUNI-ABTUNJK vector, thus expressing sfGFP evenly in the cell. |
| + | <br> |
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- | <img src="https://static.igem.org/mediawiki/2014/7/76/Oxford_Micro_title.png" style="float:right;position:relative; width:48%;" /> | + | <br><br> |
| + | <img src="https://static.igem.org/mediawiki/parts/c/cd/Part_BBa_K1446002_image_1.png" style="float:left;position:relative; width:35%;margin-right:33%;margin-bottom:3%;" /> |
| + | <img src="https://static.igem.org/mediawiki/2014/3/37/BBa_K1446002_control_image_sfGFP_nomicrocompartment_100nginducer.png" style="float:right;position:relative; width:31%;margin-bottom:6%" /> |
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- | <img src="https://static.igem.org/mediawiki/2014/f/fa/Oxford_DCMation_title.png" style="float:left;position:relative; width:48%;" /> | + | <br><br><br> |
| + | The details for the experimental procedure can be found <a href="http://parts.igem.org/Part:BBa_K1446002:Experience"> |
| + | <u>here</u></a>. |
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- | <a href="https://2014.igem.org/Team:Oxford/how_much_can_we_degrade?">
| |
- | <img src="https://static.igem.org/mediawiki/2014/e/e4/Oxford_DCMation1.png" style="float:left;position:relative; width:14%;margin-top:1.5%;" /></a>
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- | <a href="https://2014.igem.org/Team:Oxford/codon_optimisation"><img src="https://static.igem.org/mediawiki/2014/1/12/Oxford_DCMation2.png" style="float:left;position:relative; width:14%; margin-left:3.2%;margin-top:1.5%;" /></a>
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- | <a href="https://2014.igem.org/Team:Oxford/directed evolution"><img src="https://static.igem.org/mediawiki/2014/a/af/Oxford_DCMation3.png" style="float:left;position:relative; width:14%; margin-left: 3.2%;margin-top:1.5%;" /></a> | + | </div> |
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- | <a href="https://2014.igem.org/Team:Oxford/what_are_microcompartments?"><img src="https://static.igem.org/mediawiki/2014/a/ac/Oxford_Micro1.png" style="float:left;position:relative; width:14%;margin-left: 3.2%;margin-top:1.5%;" /></a> | + | </div> |
| + | </div> |
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- | <a href="https://2014.igem.org/Team:Oxford/why_do_we_need_microcompartments?">
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- | <img src="https://static.igem.org/mediawiki/2014/5/56/Oxford_Micro2.png" style="float:left;position:relative; width:14%; margin-left: 3.2%;margin-top:1.5%;" /></a>
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- | <a href="https://2014.igem.org/Team:Oxford/alternatives_to_microcompartments">
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- | <a href="https://2014.igem.org/Team:Oxford/bioremediation">
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- | <img src="https://static.igem.org/mediawiki/2014/1/11/Oxford_bioremediation_title.png" style="float:left;position:relative;width:50%; margin-top:2%;margin-left:25%;margin-right:25%;" /></a>
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- | <a href="https://2014.igem.org/Team:Oxford/Modelling"><img src="https://static.igem.org/mediawiki/2014/6/6e/Oxford_modelling_homepage_link.png" style="float:left;position:relative; width:50%; margin-top:2%;margin-left:25%;margin-right:25%;" /></a>
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- | <br><br><br>
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- | Oxford iGEM 2014
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| + | <a href="#show15" class="show wetlab-row" id="show15"><div class="wetlab"> |
| + | <h1white>Expression of the microcompartment in E.coli</h1white> |
| + | <img src="https://static.igem.org/mediawiki/2014/4/4d/Oxford_plus-sign-clip-art.png" style="float:right;position:relative; width:2%;" /> |
| + | </div></a> |
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| + | <a href="#hide15" class="hide" id="hide15"><div class="wetlab"> |
| + | <h1white>Expression of the microcompartment in E.coli</h1white></div></a> |
| + | <div class="list"> |
| + | <div class="white_news_block2"> |
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| + | Out of the seven subunits, 4 of them (B, U, N and K) are his-tagged, which allowed us to detect expression of these subunits, as shown in the western blot image below: |
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| + | <img src="https://static.igem.org/mediawiki/parts/8/8c/Westernblot_ABTUNJK.png" style="float:left;position:relative; width:70%;margin-left:15%;margin-right:25%;margin-bottom:10px;" /> |
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| + | <br> |
| + | The Western Blot clearly demonstrates successful expression of subunits B, U, N and K in E.coli, but not in Pseudomonas putida. The negative control was a Pseudomonas putida cell lysate without previous transformation. We used the standard western blot protocol described in the downloadable pdf-document at the top of this page. |
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| + | </div> |
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| + | </div> |
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- | <a href="#"><img class="heading" id="modelling" src="https://static.igem.org/mediawiki/2014/e/e0/OxigemSidebarModelling.png" style="opacity:0.8;float:left;position:fixed; top:59%; height:4.5%;" /></a>
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- | <a href="#"><img class="heading" id="achievements" src="https://static.igem.org/mediawiki/2014/4/4a/OxigemSidebarAchievements.png" style="opacity:0.75;float:left;position:fixed; top:71%; height:4%;" /></a>
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- | <a href="https://2014.igem.org/Team:Oxford/safety"><img class="heading" id="safety" src="https://static.igem.org/mediawiki/2014/3/3d/OxigemSidebarSafety.png" style="opacity:0.75;float:left;position:fixed; top:92.9%; height:4.375%;" /></a> | + | |
- | <a href="#"><img class="heading" id="dcmation" src="https://static.igem.org/mediawiki/2014/1/18/OxigemSidebarDCMation.png" style="opacity:0.8;float:left;position:fixed; top:36%; height:5.75%;" /></a>
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