Team:Evry/Notebook/CellCharacterization

From 2014.igem.org

(Difference between revisions)
Line 16: Line 16:
{{:Team:Evry/Notebook/CellCharacterization/Antibiotic_test/08-24-2014}}
{{:Team:Evry/Notebook/CellCharacterization/Antibiotic_test/08-24-2014}}
{{:Team:Evry/Notebook/CellCharacterization/Antibiotic_test/08-23-2014}}
{{:Team:Evry/Notebook/CellCharacterization/Antibiotic_test/08-23-2014}}
-
 
-
{{:Team:Evry/Notebook/CellCharacterization/Transformation/08-23-2014}}
 
-
 
{{:Team:Evry/Notebook/CellCharacterization/Antibiotic_test/08-22-2014}}
{{:Team:Evry/Notebook/CellCharacterization/Antibiotic_test/08-22-2014}}
{{:Team:Evry/Notebook/CellCharacterization/Antibiotic_test/08-21-2014}}
{{:Team:Evry/Notebook/CellCharacterization/Antibiotic_test/08-21-2014}}

Revision as of 15:14, 27 August 2014

IGEM Evry 2014

Notebook - Cell Characterization

Preparation of antibiotic stocks

Antibiotic Stock concentration Protocol
Kanamycin 25 mg/mL Weight 0.56g of Kanamycin powder, solubilization into 20mL of miliQ water.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C (aliquots of 1mL)

Streptomycin 100 mg/mL Weight 1g of Streptomycin powder, solubilization into 20mL of miliQ water.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C (aliquots of 1mL)

Amplicilin 100 mg/mL Weight 1g of Amplicilin powder, solubilization into 20mL of miliQ water.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C (aliquots of 1mL)

Tetracyclin 15 mg/mL Weight 0.6g of Tetracyclin powder, solubilization into 20mL of ethanol 50%.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C and hiden from light(aliquots of 1mL)

Chloramphenicol 34 mg/mL Weight 0.34g of Chloramphenicol powder, solubilization into 10mL of ethanol 100%.
Vortex 5min and filtration with a 200nm filter.
Stock : -20°C and hiden from light(aliquots of 1mL)