BioBricks

For developing the St²ool Project ten biobricks (composed by parts from the Registry) were picked out. All of them were simple devices consisting of a range of different promoters coupled to reporter sequences: three of these biobricks were fluorescent proteins, GFP (Green Fluorescent Protein) and RFP (Red Fluorescent Protein), under the control of constitutive promoters of different strength. We also chose lacZ and luciferase as reporters under the control of inducible promoters (temperature, antibiotic presence and IPTG). And last but not least, blue and yellow chromoproteins with pT7 as promoter were selected.

To sum up, here is the list with the ten BioBricks

<groupparts>iGEM014 Valencia_Biocampus</groupparts>

Constitutive promoter (strong)–GFP
(BBa_J23104 – BBa_E0040)

Constitutive promoter (strong)–RFP
(BBa_J23104 – BBa_E1010)

Constitutive promoter (less strong)–GFP
(BBa_J23110 – BBa_E0040)

Temperature sensitive promoter–lacZ
(BBa_K763001 [promoter only] – BBa_I732005)

pTet–lacZ
(BBa_R0040 - BBa_I732005)

pTrc–lacZ
(BBa_K649301 [promoter only] - BBa_I732005)

pTrc–Luciferase
(BBa_K649301 [promoter only] - BBa_I712019)

pT7–Blue chromoprotein
(BBa_I719005 - BBa_K864401)

pT7–Blue chromoprotein^[1]
(BBa_I719005 - BBa_K864401)

pT7–Yellow chromoprotein
(BBa_I719005 - BBa_K592010)