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Team:BostonU/FusionProteins
From 2014.igem.org
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| - | <th scope="col">Fusion proteins are analogous to tandem promoters. For our purposes, we fused multiple repressors with reporter proteins in order to check whether a transcriptional unit containing fusion proteins worked just as well as two transcriptional units (one with the repressor and the other with the reporter protein). These constructs were fused by adding unique fusion sites to their ends and then, ligating them using the <a href='https://2014.igem.org/Team:BostonU/MoClo'>Modular Cloning</a> method. The objective of making this library was to help us create standard transcriptional units that could universally be used to make intricate genetic circuits.</th> </tr> | + | <th scope="col"><br>Fusion proteins are analogous to tandem promoters. For our purposes, we fused multiple repressors with reporter proteins in order to check whether a transcriptional unit containing fusion proteins worked just as well as two transcriptional units (one with the repressor and the other with the reporter protein). These constructs were fused by adding unique fusion sites to their ends and then, ligating them using the <a href='https://2014.igem.org/Team:BostonU/MoClo'>Modular Cloning</a> method. The objective of making this library was to help us create standard transcriptional units that could universally be used to make intricate genetic circuits.</th> </tr> |
Revision as of 11:54, 21 July 2014
Fusion proteins are analogous to tandem promoters. For our purposes, we fused multiple repressors with reporter proteins in order to check whether a transcriptional unit containing fusion proteins worked just as well as two transcriptional units (one with the repressor and the other with the reporter protein). These constructs were fused by adding unique fusion sites to their ends and then, ligating them using the Modular Cloning method. The objective of making this library was to help us create standard transcriptional units that could universally be used to make intricate genetic circuits. |
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