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| <html xmlns="http://www.w3.org/1999/xhtml"> | | <html xmlns="http://www.w3.org/1999/xhtml"> |
- | <meta name="keywords" content="" /> | + | <meta name="keywords" content="iGEM,2014,Tokyo_Tech,Bank E.coli" /> |
- | <meta name="description" content="" /> | + | <meta name="description" content="iGEM2014 Wiki pages" /> |
| <meta http-equiv="content-type" content="text/html; charset=utf-8" /> | | <meta http-equiv="content-type" content="text/html; charset=utf-8" /> |
| <title>Tokyo_Tech</title> | | <title>Tokyo_Tech</title> |
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| <div id="header"> | | <div id="header"> |
| <div id="logo"> | | <div id="logo"> |
- | <img src="https://static.igem.org/mediawiki/2014/thumb/b/b7/Tokyo_Tech_Logo.png/613px-Tokyo_Tech_Logo.png" height="150" /></h1></div>
| + | |
- | <div align="right"><img src="https://static.igem.org/mediawiki/2014/b/b4/Tokyo_Tech_Logo2.png" height="150" /></div>
| + | <a href="https://2014.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2014/thumb/8/84/Tokyo_Tech_iGEM_logo.png/794px-Tokyo_Tech_iGEM_logo.png" width="90" /></a></div> |
| </div> | | </div> |
| <!-- end #header --> | | <!-- end #header --> |
| | | |
| + | <a href="http://www.titech.ac.jp/english/index.html" id="logo-link"></a> |
| + | |
| <div id="menu"> | | <div id="menu"> |
| <ul> | | <ul> |
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| <li><a href="#">Experiment</a> | | <li><a href="#">Experiment</a> |
| <ul> | | <ul> |
- | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/C4HSL-dependent_3oxoC12HSL_production" style="width:400px; margin-left:-135px;">C4HSL-dependent 3oxoC12HSL production</a></li> | + | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Plux and Prhl reporter assay" style="width:400px; margin-left:-135px;">Plux and Prhl reporter assay</a></li> |
- | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/3oxoC12HSL-dependent_C4HSL_production" style="width:400px; margin-left:-135px;">3oxoC12HSL-dependent C4HSL production</a></li> | + | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay" style="width:400px; margin-left:-135px;">Improved Prhl reporter assay</a></li> |
- | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Symbiosis_confirmation_by_co-culture" style="width:400px; margin-left:-135px;">Symbiosis confirmation by co-culture </a></li> | + | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/C4HSL-dependent_3OC12HSL_production" style="width:400px; margin-left:-135px;">C4HSL-dependent 3OC12HSL production</a></li> |
- | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Prhl_reporter_assay" style="width:400px; margin-left:-135px;">Prhl reporter assay </a></li> | + | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/3OC12HSL-dependent_C4HSL_production" style="width:400px; margin-left:-135px;">3OC12HSL-dependent C4HSL production</a></li> |
- |
| + | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Experiment/Symbiosis_confirmation_by_co-culture" style="width:400px; margin-left:-135px;">Mutualism Confirmation ~Co-culture Assay~</a></li> |
| </ul> | | </ul> |
| </li> | | </li> |
| <li><a href="#">Modeling</a> | | <li><a href="#">Modeling</a> |
- | <ul>
| + | <ul> |
- | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Modeling1" style="width:260px; margin-left:-63px;">なぞなぞ</a></li>
| + | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Overview" style="width:400px; margin-left:-135px;">Overview</a></li> |
- | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Modeling2" style="width:260px; margin-left:-63px;">Parameter sensitivity analysis</a></li>
| + | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Growth Conditions For Company And Customer" style="width:400px; margin-left:-135px;">Growth Conditions For Company And Customer</a></li> |
- | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Modeling3" style="width:260px; margin-left:-63px;">The effect of economic wave</a></li>
| + | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Analysis of C4HSL-dependent Switch" style="width:400px; margin-left:-135px;">Analysis of C4HSL-dependent Switch</a></li> |
- | </ul>
| + | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Modeling/Economic Wave" style="width:400px; margin-left:-135px;">Economic Wave</a></li> |
| + | </ul> |
| </li> | | </li> |
| <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">Parts</a></li> | | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">Parts</a></li> |
- | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices" style="height:45px; padding-top:4px;">Policy and Practices</a></li> | + | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices" style="height:50px; padding-top:3px;">Policy and Practices</a></li> |
| <li class="current_page_item"><a href="#">More</a> | | <li class="current_page_item"><a href="#">More</a> |
| <ul> | | <ul> |
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| <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Judging_Form" style="width:240px; margin-left:-53px;">Judging Form</a></li> | | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Judging_Form" style="width:240px; margin-left:-53px;">Judging Form</a></li> |
| <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Sponsors" style="width:240px; margin-left:-53px;">Sponsors</a></li> | | <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Sponsors" style="width:240px; margin-left:-53px;">Sponsors</a></li> |
- | <li><a href="https://igem.org/Team.cgi" style="width:240px; margin-left:-53px;">Official Team Page</a></li> | + | <li><a href="https://igem.org/Team.cgi?id=1529" style="width:240px; margin-left:-53px;">Official Team Page</a></li> |
| </ul> | | </ul> |
| </li> | | </li> |
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| <div id="page"> | | <div id="page"> |
| <div id="content_normal"> | | <div id="content_normal"> |
- | <div class="post">
| + | <div class="post"> |
- | <h2 class="title"><a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practice">Attribution and Contribution </a></h2>
| + | <h2 class="title">Attribution and Contribution</h2> |
| | | |
- | <span class="meta">We could not reach this point without everyone </span> | + | <span class="meta">We could not reach this point without anyone </span> |
| <div class="entry-long"> | | <div class="entry-long"> |
| + | |
| + | <div id="gototop"><a href="#"><img src="https://static.igem.org/mediawiki/2014/5/55/Tokyo_Tech_Go-to-top-icon.png" height="50" /></a></div> |
| + | |
| <table width="900" border="0"> | | <table width="900" border="0"> |
| <tr> | | <tr> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">We are proud to say that all the ideas we worked on this summer were proposed by undergraduate members of our team Tokyo_Tech 2014. Moreover, the whole experimental data created by our team comes from experiments all carried out by undergraduates. The ideas of "Fertilizer" and "Bank E. coli" were proposed by Riku Shinohara and Naoto Takahashi. Modeling related to these two projects were done by Takuya Kato and Ye Kai. We want to thank every advisors and instructors who helped us discussing these ideas and guided us in the experiments.</td> | + | <td colspan="2"><p class="info-18">We are proud to say that all the ideas we worked on this year were proposed by undergraduate members of our team Tokyo_Tech 2014. Moreover, the whole experimental data created by our team comes from experiments all carried out by undergraduates. The project "Bank <i>E. coli</i>" was proposed by Naoto Takahashi. Modeling related to this project was done by Takuya Kato and Ye Kai. We want to thank every advisers and instructors who helped us with discussing these ideas and guided us in the experiments.</p></td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td class="head">Student team members </td>
| + | <td class="head">Student team members </td> |
| <td> </td> | | <td> </td> |
| </tr> | | </tr> |
- | <tr> | + | |
- | <td colspan="2">Our undergraduate members carried out all the experiments! </td>
| + | |
- | </tr>
| + | |
| <tr> | | <tr> |
| <td colspan="2"> </td> | | <td colspan="2"> </td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">Performing all cloning (transformation, culture cells, mini prep):</td> | + | <td colspan="2" class="info-18">All cloning (transformation, culture cells, mini prep):</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2"><ul class="info"> | + | <td colspan="2"><p class="info"> - Yuta Kino, Yuya Nakamura, Kohdai Hibi with helping of other team members </p> </td> |
- | <li> -Yuta Kino, Yuya Nakamura, Kohdai Hibi with helping of other students </li>
| + | |
- | </ul></td>
| + | |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">Performing Bank <em>E. coli</em> assay: </td> | + | <td colspan="2" class="info-18">Parts construction (restriction digest, ligation):</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info"> - | + | <td colspan="2"><p class="info"> - Yuta Kino, Yuya Nakamura, Kohdai Hibi </p></td> |
- | <em>Miyabi Hishinuma</em>, <em>Ryu Gyomon, Xinan Wang</em></td>
| + | |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">Performing Fertilizer assay (experiment using DAPI, Malachite Green): </td> | + | <td colspan="2" class="info-18">AHL-dependent gene expression assay: </td> |
| </tr> | | </tr> |
| + | |
| <tr> | | <tr> |
- | <td colspan="2"><ul class="info"> | + | <td colspan="2"><p class="info"> - Shoko Suzuki, Naoto Takahashi, Keina Matsumura</p></td> |
- | <li> -
| + | |
- | <em>Shoko Suzuki, </em><em>Keina Matsumura, Ay</em><em>aka Murase</em></li>
| + | |
- | </ul></td>
| + | |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">Performing parts construction (restriction digest, ligation): </td> | + | <td colspan="2" class="info-18">Co-culture assay: </td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2"><ul class="info"> | + | <td colspan="2"><p class="info"> - Yuya Nakamura, Gyomon Ryu</p></td> |
- | <li> -
| + | |
- | <em>Yuta Kino</em>,
| + | |
- | <em>Yuya Nakamura, </em><em>Kohdai Hibi</em></li>
| + | |
- | </ul></td>
| + | |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">Modeling (simulation of the genetic circuit)</td> | + | <td colspan="2" class="info-18">Improved rhl promoter assay: </td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info"> - | + | <td colspan="2"><p class="info"> - Naoto Takahashi, Keina Matsumura, Ayaka Murase </p></td> |
- | <em>Takuya Kato,</em>
| + | |
- | <em>Ye Kai</em> </td>
| + | |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2"><div align="center" class="title-small"> Contribution</div></td> | + | <td colspan="2" class="info-18">Modeling (simulation of the genetic circuit):</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2"> </td> | + | <td colspan="2" class="info"><p class="info"> - Takuya Kato, Kai Ye </p></td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="head">General Support</td> | + | <td colspan="2"> </td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2"> </td> | + | <td colspan="2" class="info-18">Wiki and slide design: </td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">- Prof. Kiga let us use his lab for all of our experiments. He also has led many Tokyo Tech teams to the excellent results in iGEM since this competition has started.</td> | + | <td colspan="2" class="info"><p class="info"> - Paniti Achararit, Kai Ye, Riku Shinohara </p></td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">- Prof. Yamamura and Prof. Konagaya supported us in many ways to give us a better researching environment. </td> | + | <td colspan="2" class="info-18">Policy and Practices: </td> |
| + | </tr> |
| + | <tr> |
| + | <td colspan="2" class="info"><p class="info"> - Miyabi Hishinuma, Ayaka Murase with helping of other team members </p></td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">- Prof. Ayukawa supported us in our experiment throughout the preparations for iGEM.</td> | + | <td colspan="2"><div align="center" class="title-small">Contribution</div></td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">- Mr. Sekine and Mr. Amikura gave us the helpful advice for playing an active part in iGEM competition. </td> | + | <td colspan="2" class="head">General Support</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">- Promega provided us the part of restriction enzyme we used without any charge.</td> | + | <td colspan="2" class="info-18">Prof. Kiga let us use his lab for all of our experiments. He also has led many Tokyo Tech teams to the excellent results in iGEM since this competition has started.</td> |
| </tr> | | </tr> |
| + | |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18"> </td> | + | <td colspan="2" class="info-18">Prof. Yamamura and Prof. Konagaya supported us in many ways to give us a better researching environment. </td> |
| </tr> | | </tr> |
| + | |
| <tr> | | <tr> |
- | <td colspan="2" class="head">Instrumental Analysis Support</td> | + | <td colspan="2" class="info-18">Prof. Ayukawa supported us in all of our experiments throughout the preparations for iGEM. |
| + | </td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2"> </td>
| + | <td colspan="2" class="info-18">Promega KK provided us a part of the restriction enzyme we used without any charge. |
- | </tr>
| + | </td> |
- | <tr>
| + | |
- | <td colspan="2" class="info-18">- Biomaterial Analysis Center, Technical Department, sequenced the sequence of plasmids which we had asked for. </td> | + | |
| </tr> | | </tr> |
| + | |
| <tr> | | <tr> |
| <td colspan="2" class="info-18"> </td> | | <td colspan="2" class="info-18"> </td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="head">Human Practice Support</td> | + | <td colspan="2" class="head">Instrumental Analysis Support</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">- Mr. Katayama who is working in Tokyo Institute of Technology Graduate School of Innovation Management gave us his opinion on issues of environmental and beneficiary survey of our projects.</td> | + | <td colspan="2" class="info-18">Biomaterial Analysis Center, Technical Department, checked the sequence of the plasmids which we had asked. </td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2"> </td> | + | <td colspan="2" class="info-18"> </td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">- | + | <td colspan="2" class="head">Policy and Practice Support</td> |
- | Mr. Kawabata who is working in JA ZENNOH CHIBA advised what problems and benefits will be occurred if our project was applied.</td>
| + | |
| </tr> | | </tr> |
| <tr> | | <tr> |
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| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">- | + | <td colspan="2" class="info-18">Tomoya Saito, who is working for National Institute of Public Health, gave us a lecture on dual-use technologies, and gave us useful advice about <a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices/Our_question_about_biosafety">biosafety.</a></td> |
- | Takeda Science Foundation introduced us in their book.</td>
| + | |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2"> </td> | + | <td colspan="2" class="info-18">Hiromichi Ando arranged <a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices#MUSE">MUSE TALK</a> and let us present there.</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">- | + | <td colspan="2" class="info-18">Akihiro Hiyoshi, who is the principal of <a href="https://2014.igem.org/Team:Tokyo_Tech/Policy_and_Practices#Visit">Ohyamaguchi Elementary School</a>, gave us a chance to communicate with students.</td> |
- | Mr. Sato (a teacher of Shinjuku high school) proposed the experimental classroom, and led us.</td>
| + | </tr> |
- | </tr> | + | |
| <tr> | | <tr> |
- | <td colspan="2"> </td> | + | <td colspan="2" class="info-18"> </td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">- | + | <td colspan="2" class="head">Presentation Adviser</td> |
- | Ms. Inoue (a teacher of Sagamihara high school) and Ms. Miyamoto (a teacher of Toin Gakuen) collected lots of high school students to our experimental classroom.</td>
| + | |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18"> </td> | + | <td colspan="2"> </td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="head">And last but not least</td> | + | <td colspan="2" class="info-18">We would like to thank all the people who came to check our presentations and gave us useful feedbacks:</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2"> </td> | + | <td colspan="2"><p class="info"><strong>Professor: </strong>Masaaki Wachi, Hiroshi Iwasaki, Masaaki Kotera, Nobuhiro Hayashi, Nobutaka Nakashima, Satoshi Nakamura, Shunichiro Ogura, Hideki Taguchi, Masahiro Takinoue, Toshiaki Fukui, Fumio Arisaka, Yasunori Tanji, Hirokazu Urabe, Hisakazu Mihara, Yasunori Aizawa, Hiroyuki Ohta, Kumiko Sogawa, Masayuki Yamamura,Takao Terano, Robert F. Whitter, Robert Cox</td> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td colspan="2" class="info-18">We would like to thank everyone who came to our progress presentations and gave useful feedback and input:</td> | + | <td colspan="2"><p class="info"><strong>Veteran: </strong>Ryota Fujiwara, Toshiki Hashimoto, Taku Nakayama, Nobuaki Yasuo, Shinya Suzuki, Hiroshi Fujimoto, Sara Ogino, Shunta Suzuki</td> |
- | </tr>
| + | |
- | <tr>
| + | |
- | <td colspan="2" class="info"> - <em>Fumio Arisaka, Toshiaki Fukui, Takeshi Hata, Junji Hirota, Hiroshi Iwasaki, Satoshi Nakamura, Shunichiro Ogura, Hideki Taguchi, Masahiro Takinoue, Yasunori Tanji, Hirokazu Urabe, Masaaki Wachi, Robert F. Whittier, Daniel Berrar, Mai Miura, Taku Nakayama, Goshi Sugano, Naoki Tsukuda and Nobuaki Yasuo</em></td>
| + | |
| </tr> | | </tr> |
| </table> | | </table> |
| <p> </p> | | <p> </p> |
- | </div>
| + | </div> |
- | </div>
| + | </div> |
| </div> | | </div> |
| <!-- end #content --> | | <!-- end #content --> |