Team:Glasgow/Parts
From 2014.igem.org
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<tr id="fliCH"><th colspan="2">Contain FliC</th></tr> | <tr id="fliCH"><th colspan="2">Contain FliC</th></tr> | ||
<tr> | <tr> | ||
- | <td></td> | + | <td>BBa_K1463600</td> |
- | <td> | + | <td>The basic FliC part</td> |
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</tr> | </tr> | ||
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+ | <tr><td>BBa_K1463601</td> | ||
+ | <td>Composite of B0034 (RBS), K1463600</td></tr> | ||
+ | <tr><td>BBa_K1463602</td> | ||
+ | <td>Composite of J23116, B0032, B0034, K1463600</td></tr> | ||
+ | <tr><td>BBa_K1463603 </td> | ||
+ | <td>Composite of J23106, B0032, B0034, K1463600</td></tr> | ||
- | </ | + | <tr><td>BBa_K1463604</td> |
+ | <td>Composite of J23100, B0032, B0034, K1463600</td</tr> | ||
+ | <tr id="motAH"><th colspan="2">Contain MotA</th></tr> | ||
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+ | </table> | ||
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+ | <td></td> | ||
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</ul> | </ul> |
Revision as of 22:29, 17 October 2014
Biobricks
The following table lists the biobricks we created. Many of these have additional characterisation information, available on their respective Registry pages.
We have also put together a Motility Parts Collection
Some important parts:
- BBa_B0032 - Weak Ribosome Binding Site
- BBa_B0034 - Strong Ribosome Binding Site
- BBa_J61002 - Plasmid Vector
- BBa_J23xxx - Promoter
Please note that all parts highlighted in RED have been submitted and received by the registry.
Click here to editBiobrick Number | Type | Description | Designer | Length (BP) | Design Notes |
---|---|---|---|---|---|
BBa_K1463000 | Composite | Switch Biobrick with RFP and GFP. BBa_K1463520, BBa_K1463560, BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0034, BBa_E0040 | iGEM14_Glasgow | 1744 | |
BBa_K1463001 | Composite | Switch Biobrick with just GFP. BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0034, BBa_E0040 | iGEM14_Glasgow | 1013 | |
BBa_K1463002 | Composite | Switch Biobrick with just GFP weaker RBS. BBa_K1463040, BBa_K1463030, BBa_K1463501, BBa_B0010, BBa_K1463041, BBa_B0032, BBa_E0040 | iGEM14_Glasgow | 1014 | |
BBa_K1463030 | Basic | Switch nucleotide spacer | iGEM14_Glasgow | 11 | |
BBa_K1463040 | Basic | φC31 attP site | iGEM14_Glasgow | 54 | |
BBa_K1463041 | Basic | φC31 attB site | iGEM14_Glasgow | 55 | |
BBa_K1463050 | Composite | K1463040, K1463030, K1463501, B0010, K1463041 | iGEM14_Glasgow | 267 | |
BBa_K1463100 | GvpA RBS | iGEM14_Glasgow | 8 | ||
BBa_K1463205 | Composite | B0034, K737016 | iGEM14_Glasgow | 237 | |
BBa_K1463207 | Composite | J61002, J23100, B0034, K737016 | iGEM14_Glasgow | 3226 | |
BBa_K1463210 | Composite | K1463100, K737016 | iGEM14_Glasgow | 233 | |
BBa_K1463225 | Composite | GvpC(K737017) and B0032 | iGEM14_Glasgow | 523 | |
BBa_K1463340 | Composite | B0034, K737016, B0032, K737017 | iGEM14_Glasgow | 764 | |
BBa_K1463345 | Composite | J61002, J23100, B0034, K737016, B0032, K737017 | iGEM14_Glasgow | 3767 | |
BBa_K1463350 | Composite | K1463100, K737016, B0032, K737017 | iGEM14_Glasgow | 764 | |
BBa_K1463400 | φC31 Gp3 | iGEM14_Glasgow | 738 | ||
BBa_K1463401 | Composite | B0034, K1463400 | iGEM14_Glasgow | 756 | |
BBa_K1463501 | Promoter | J23100 promoter in reverse | iGEM14_Glasgow | 38 | |
BBa_K1463520 | Basic | E1010 RFP in reverse | iGEM14_Glasgow | 705 | |
BBa_K1463560 | Basic | Strong reverse RBS | iGEM14_Glasgow | 10 | |
BBa_K1463600 | Basic | FliC | iGEM14_Glasgow | 1497 | |
BBa_K1463601 | Composite | B0034, K1463600 | iGEM14_Glasgow | 1515 | |
BBa_K1463602 | Composite | J23116, B0032, B0034, K1463600 | iGEM14_Glasgow | 1579 | |
BBa_K1463603 | Composite | J23106, B0032, B0034, K1463600 | iGEM14_Glasgow | 1579 | |
BBa_K1463604 | Composite | J23100, B0032, B0034, K1463600 | iGEM14_Glasgow | 1579 | |
BBa_K1463700 | Basic | MotA | 888 | ||
BBa_K1463701 | Composite | B0032, K1463700 | iGEM14_Glasgow | 907 | |
BBa_K1463702 | Composite | J23100, B0032, K1463700 | iGEM14_Glasgow | 950 | |
BBa_K1463703 | Composite | J23103, B0032, K1463700 | iGEM14_Glasgow | 950 | |
BBa_K1463704 | Composite | J23106, B0032, K1463700 | iGEM14_Glasgow | 950 | |
BBa_K1463705 | Composite | J23112, B0032, K1463700 | iGEM14_Glasgow | 950 | |
BBa_K1463706 | Composite | J23116, B0032, K1463700 | iGEM14_Glasgow | 950 | |
BBa_K1463750 | Basic | MotB | iGEM14_Glasgow | 927 | |
BBa_K1463751 | Composite | B0032, K1463750 | iGEM14_Glasgow | 946 | |
BBa_K1463752 | Composite | J23100, B0032, K1463750 | iGEM14_Glasgow | 989 | |
BBa_K1463753 | Composite | J23103, B0032, K1463750 | iGEM14_Glasgow | 989 | |
BBa_K1463754 | Composite | J23106, B0032, K1463750 | iGEM14_Glasgow | 989 | |
BBa_K1463755 | Composite | J23112, B0032, K1463750 | iGEM14_Glasgow | 989 | |
BBa_K1463756 | Composite | J23116, B0032, K1463750 | iGEM14_Glasgow | 989 | |
BBa_K1463770 | Composite | J23103, B0032, K1463700, B0032, K1463750 | iGEM14_Glasgow | 1904 | |
BBa_K1463771 | Composite | J23112, B0032, K1463700, B0032, K1463750 | iGEM14_Glasgow | 1904 | |
BBa_K1463772 | Composite | J23106, B0032, K1463700, B0032, K1463750 | iGEM14_Glasgow | 1904 | |
BBa_K1463773 | Composite | J23116, B0032, K1463700, B0032, K1463750 | iGEM14_Glasgow | 1904 | |
BBa_K1463805 | Composite | J61002, J23103, E1010 | iGEM14_Glasgow | 3703 | |
BBa_K1463806 | Composite | J61002, J23106, E1010 | iGEM14_Glasgow | 3703 | |
BBa_K1463807 | Composite | J61002, J23112, E1010 | iGEM14_Glasgow | 3703 | |
BBa_K1463808 | Composite | J61002, J23116, E1010 | iGEM14_Glasgow | 3703 |
Motility Parts Collection
The motility parts collection is the culmination of our work on bacterial motility genes - MotA, MotB and FliC. Our project plan required that, for the bacteria to float, these genes would need switched off. Therefore, work had to be done to:
- Knock out the genes from the bacteria
- Be able to recover the motility
- Make the parts suitable for placement on one side of the recombinase switch
Part Number | Description |
---|---|
Contain FliC | |
BBa_K1463600 | The basic FliC part |
BBa_K1463601 | Composite of B0034 (RBS), K1463600 |
BBa_K1463602 | Composite of J23116, B0032, B0034, K1463600 |
BBa_K1463603 | Composite of J23106, B0032, B0034, K1463600 |
BBa_K1463604 | Composite of J23100, B0032, B0034, K1463600 |
Contain MotA |