Team:UCL/Tests

From 2014.igem.org

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   <img src="https://static.igem.org/mediawiki/2014/2/21/OExperiments_Bannero.jpg" width="100%" height="100%" alt="Experiments" />
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   <img src="https://static.igem.org/mediawiki/2014/0/07/OAchievements_Bannero.jpg" width="100%" height="100%" alt="Achievements" />
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<ul class="tabs">
<ul class="tabs">
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     <li><a href="#view1">Stage 1</a></li>
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     <li><a href="#view1">Bronze</a></li>
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     <li><a href="#view2">Stage 2</a></li>
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     <li><a href="#view2">Silver</a></li>
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     <li><a href="#view3">Stage 3</a></li>
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     <li><a href="#view3">Gold</a></li>
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     <li><a href="#view4">Stage 4</a></li>
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     <li><a href="#view4">Special Prizes</a></li>
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    <li><a href="#view5">Stage 5</a></li>
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    <li><a href="#view6">Stage 6</a></li>
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    <li><a href="#view7">Stage 7</a></li>
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    <li><a href="#view8">Stage 8</a></li>
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<div class="tabcontents">
<div class="tabcontents">
<!--- This is the overview section --->
<!--- This is the overview section --->
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<div id="view1"><div class="textTitle"><h4>Stage 01: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit</h4></div><br>
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<div id="view1"><div class="textTitle"><h4>Bronze Achieved!</h4></div><br>
<!-- <a data-tip="true" class="top large" data-tip-content="Here's Tanel doing some pipetting in our lab!" href="javascript:void(0)" style="width: 25%;float: right;margin-left:2%"><img src="https://static.igem.org/mediawiki/2014/c/c9/UCLTANELPIPETTING.JPG" style="max-width: 100%;"></a> -->
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<div><strong>Protocols&nbsp;&nbsp;</strong>
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<p>EDO WRITE THINGS HERE</p><br>
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    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
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    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
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    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a>
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    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">digest</span></a>
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    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">gel</span></a></div>
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<p>We began our project by identifying a range of BioBrick parts present in the iGEM 2014 distribution kit which we required as part of our cloning strategy. These parts primarily consisted of both constituitive and inducible promoter systems with ribosome binding sites which we could then use in conjunction with our azo-reductase BioBricks to assemble a functional azo dye degrading gene. We also decided that we would use the Red Florescent Protein expressing BioBrick as a control for any further transformation experiments. As the level of DNA present within each plate of the distribution kit is insufficient to perform digest and ligation reactions on it was necessary to transform each of these plasmids into our NEB5alpha competent cells. After growing our transformed cells overnight we then mini-prepped each of them to obtain BioBrick plasmids at suitable concentrations for future experiments.</p><br>
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         <thead>
         <thead>
             <tr>
             <tr>
                 <th>  </th>
                 <th>  </th>
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                 <th> Registry ID </th>
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                 <th> Task </th>
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                 <th> Name / Function </th>
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                 <th> Where am I on wiki? </th>
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                <th> Source </th>
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                 <th> Where am I on iGEM? </th>
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                 <th> Size </th>
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             </tr>
             </tr>
         </thead>
         </thead>
         <tbody>
         <tbody>
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            <!--Lisbon plasmids-->
             <tr>
             <tr>
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                 <td> <center>U</center> </td>
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                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
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                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K314103">BBa_K314103</a> </td>
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                 <td> &nbsp;Team registration </td>
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                 <td> &nbsp;IPTG-inducible LacI Expression Cassette</td>
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                 <td> &nbsp;<a href="/Team:UCL/Humans/Team">Meet our Team!</a> </td>
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                 <td> &nbsp;Spring 2014 BioBrick Distribution. Plate 1, Well 4D.</td>
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                 <td> &nbsp;<a href="https://igem.org/Team.cgi?id=1336">Team Roster!</a> </td>
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                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K314103">1638 bp</a> </td>
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             </tr>
             </tr>
             <tr>
             <tr>
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                 <td> <center>T</center> </td>
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                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
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                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_J04450">BBa_J04450</a> </td>
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                 <td> &nbsp;Complete Judging form </td>
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                 <td> &nbsp;RFP Coding Device </td>
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                 <td> &nbsp;<a href="/Team:UCL/Project/Achievements">Our Achievements!</a> </td>
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                 <td> &nbsp;Spring 2014 BioBrick Distribution. Plate 4, Well 4B. </td>
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                 <td> &nbsp;<a href="https://igem.org/2014_Judging_Form?id=1336">iGEM Judging form!</a> </td>
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                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_J04450">1069 bp</a> </td>
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             </tr>
             </tr>
             <tr>
             <tr>
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                 <td> <center>T</center> </td>
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                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
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                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_R0010">BBa_R0010</a> </td>
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                 <td> &nbsp;Team Wiki </td>
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                 <td> &nbsp;IPTG-inducible LacI Promoter</td>
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                 <td> &nbsp;<a href="/Team:UCL">Team wiki!</a> </td>
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                 <td> &nbsp;Spring 2014 BioBrick Distribution. Plate 3, Well 4G.</td>
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                 <td> &nbsp;<a href="www.goodbyeazodye.org.uk">See us here also!</a> </td>
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                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_R0010">200 bp</a> </td>
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             </tr>
             </tr>
             <tr>
             <tr>
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                 <td> <center>T</center> </td>
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                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
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                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a> </td>
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                 <td> &nbsp;We will present our project poster and talk at the iGEM Giant Jamboree, 30 October - 3 November 2014 </td>
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                 <td> &nbsp;Ribosomal Binding Site (RBS)</td>
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                 <td> &nbsp;<a href="/Team:UCL">...</a> </td>
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                 <td> &nbsp;Spring 2014 BioBrick Distribution. Plate 4, Well 1N.</td>
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                 <td> &nbsp;<a href=" ">...</a> </td>
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                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_B0034">12 bp</a> </td>
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             </tr>
             </tr>
             <tr>
             <tr>
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                 <td> <center>T</center> </td>
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                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
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                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K518012">BBa_K518012</a> </td>
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                 <td> &nbsp;Use of new/improved application of previously existing standard BioBrick Part or Device, and submitted to the iGEM Registry </td>
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                 <td> &nbsp;RBS + RFP + double Terminator</td>
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                 <td> &nbsp;<a href="/Team:UCL/Project/Biobricks">...</a> </td>
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                 <td> &nbsp;Spring 2014 BioBrick Distribution. Plate 1, Well 18C.</td>
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                 <td> &nbsp;<a href=" ">...</a> </td>
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                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K518012">828 bp</a> </td>
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             </tr>
             </tr>
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        </tbody>
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    </table>
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    </font>
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</div>
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<!--- This is the first biobrick --->
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<div id="view2"><div class="textTitle"><h4>Silver Achieved!</h4></div><br>
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<p>EDO WRITE THINGS HERE</p><br>
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        <thead>
             <tr>
             <tr>
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                 <td> <center>N</center> </td>
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                 <th> </th>
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                 <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_K206000">BBa_K206000</a> </td>
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                 <th> Task </th>
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                 <td> &nbsp;pBAD Strong Promoter</td>
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                 <th> Where am I on wiki? </th>
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                 <td> &nbsp;Spring 2014 BioBrick Distribution. Plate 3, Well 14A.</td>
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                 <th> Where am I on iGEM? </th>
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                <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_K206000">130 bp</a> </td>
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             </tr>
             </tr>
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        </thead>
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        <tbody>
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            <!--Lisbon plasmids-->
             <tr>
             <tr>
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                 <td> <center>! N</center> </td>
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                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e8/UCL_Silver-metal-star.jpg" width="25px"></center> </td>
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                <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_R0011">BBa_R0011</a> </td>
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                 <td> &nbsp;Experimental validation that at least one of our new Biobrick Part(s) or Device(s) works as expected. </td>
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                 <td> &nbsp;LacI-Regulated, Lambda pL Hybrid Promoter</td>
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                 <td> &nbsp;<a href="/Team:UCL/Humans/Team">Meet our Team!</a> </td>
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                 <td> &nbsp;Spring 2014 BioBrick Distribution. Plate 2, Well 6D.</td>
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                 <td> &nbsp;<a href="https://igem.org/Team.cgi?id=1336">Team Roster!</a> </td>
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                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_R0011">55 bp</a> </td>
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             </tr>
             </tr>
             <tr>
             <tr>
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                 <td> <center>! N</center> </td>
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                 <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e8/UCL_Silver-metal-star.jpg" width="25px"></center> </td>
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                 <td> &nbsp;<a href="http://parts.igem.org/Part:BBa_B0012">BBa_B0012</a> </td>
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                <td> &nbsp;Characterisation of working Biobrick(s) documented in the "Main Page" section of its Part's/Device's Registry Entry. </td>
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                 <td> &nbsp;Transcription Terminator for E. coli RNA Polymerase</td>
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                 <td> &nbsp;<a href="/Team:UCL/Project/Achievements">Our Achievements!</a> </td>
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                 <td> &nbsp;Spring 2014 BioBrick Distribution. Plate 2, Well 2B.</td>
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                <td> &nbsp;<a href="https://igem.org/2014_Judging_Form?id=1336">iGEM Judging form!</a> </td>
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                 <td> &nbsp;<a href="/Team:UCL/Science/Sequences#BBa_B0012">41 bp</a> </td>
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            </tr>
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            <tr>
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                <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e8/UCL_Silver-metal-star.jpg" width="25px"></center> </td>
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                <td> &nbsp;New part submitted to iGEM Parts Registry (adhering to the iGEM Registry guidelines). </td>
 +
                <td> &nbsp;<a href="/Team:UCL">Team wiki!</a> </td>
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                 <td> &nbsp;<a href="www.goodbyeazodye.org.uk">See us here also!</a> </td>
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            </tr>
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            <tr>
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                <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e8/UCL_Silver-metal-star.jpg" width="25px"></center> </td>
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                 <td> &nbsp;Implications of our project to the environment, security, safety and ethics, and/or ownership and sharing. Also, description of one or more ways these, or broader, implications have been taken into consideration in the design and execution of our project. </td>
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                 <td> &nbsp;<a href="/Team:UCL">...</a> </td>
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                <td> &nbsp;<a href=" ">...</a> </td>
             </tr>
             </tr>
         </tbody>
         </tbody>
     </table>
     </table>
     </font>
     </font>
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    <div><font size="1">Note: U = Used in experiments; T = Used for testing purposes but not for making BioBrick Devices; N = Transformed from Distribution Kits, but not used in experiments; ! = Problematic parts (see Parts Registry), were not used.</font></div>
 
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<!--- This is the first biobrick --->
 
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<div id="view2"><div class="textTitle"><h4>Stage 02: Identification of useful genes for making new BioBricks</h4></div><br>
 
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<strong>Extraction of Bacillus subtilis genomic DNA</strong>
 
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<div><strong>Protocols&nbsp;&nbsp;</strong>
 
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    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">DNA extraction</span></a></div>
 
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<p>Our literature search identified a number of bacterial species that have been proven to degrade azo dye compounds including <i>B. subtilis</i> and <i>P. aeruginosa</i>.  We were able to obtain a <i>B. subtilis</i> strain for use in our project from ?.  We extracted the genomic DNA from this strain using a Promega Wizard Genomic DNA extraction kit so that we could subsequently amplify the azo-reducatase gene (AzoR1) and create our first azo-reductase BioBrick.  After completing the genomic DNA extracton we ran a gel to show that we had successfully extracted the <i>B. subtilis</i> genomic DNA.</p><br>
 
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</div>
</div>
<!--- This is the second biobrick --->
<!--- This is the second biobrick --->
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<div id="view3"><div class="textTitle"><h4>Stage 03: Transforming E. coli with azo-reductase plasmids</h4></div><br>
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<div id="view3"><div class="textTitle"><h4>Gold Achieved!</h4></div><br>
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<!-- This is the biobrick image -->
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<a data-tip="true" class="top large" data-tip-content="This diagram explains the basic construct of the BioBrick, the only part that changes is the selected function itself; in this case attributed to BBa_K1336001." href="javascript:void(0)" style="width: 30%;float: right;margin-left:2%"><img src="https://static.igem.org/mediawiki/2014/5/5d/UCLBBAzo1b6.png" style="max-width: 100%;"></a>
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<!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately-->
<!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately-->
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<div><strong>Protocols&nbsp;&nbsp;</strong>
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<p>EDO WRITE THINGS HERE</p><br>
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     <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
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     <font size="2">
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
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    <table width="100%" height="auto">
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    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a></div>
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        <thead>
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    <p>We were gratefully provided with a set of five plasmids from the <a href="http://www.itqb.unl.pt/martins">Microbial & Enzyme Technology Lab</a> led by Dr Lígia O. Martins at the Universidade Nova de Lisboa. They are currently researching how azo-dye degrading enzymes function and are keen to collaborate with us. These plasmids contained a number of genes encoding azo-dye degrading enzymes from both <i>B. subtilis</i> and <i>P. putida</i> including mutated forms found to exhibit enhanced degradation activity.  As the DNA concentration of the plasmids we were sent was insufficient to perform PCR amplification on we transformed each of these plasmids into our <i>E. coli</i> NEB5alpha derivative competent cells.  After growing the cells overnight we then mini-prepped each of them to obtain plasmids at sufficient concentrations for future experimental work.</p>
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            <tr>
 +
                <th>  </th>
 +
                <th> Task </th>
 +
                <th> Where am I on wiki? </th>
 +
                <th> Where am I on iGEM? </th>
 +
            </tr>
 +
        </thead>
 +
        <tbody>
 +
            <!--Lisbon plasmids-->
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
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                <td> &nbsp;Improve the function OR characterization of an existing BioBrick Part or Device, document this in the "Experience" section of its entry in the iGEM Registry, and submit the part to the iGEM Parts Registry. </td>
 +
                <td> &nbsp;<a href="/Team:UCL/Humans/Team">Meet our Team!</a> </td>
 +
                <td> &nbsp;<a href="https://igem.org/Team.cgi?id=1336">Team Roster!</a> </td>
 +
            </tr>
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Help any registered iGEM team from another school or institute, e.g. characterising a part, debugging a construct, or modelling/simulating their system. </td>
 +
                <td> &nbsp;<a href="/Team:UCL/Project/Achievements">Our Achievements!</a> </td>
 +
                <td> &nbsp;<a href="https://igem.org/2014_Judging_Form?id=1336">iGEM Judging form!</a> </td>
 +
            </tr>
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Describe a novel approach of our team to help us and others consider the implications/aspects of the design and outcomes of synthetic biology efforts; justifying its novelty and how this approach might be adapted and scaled for others to use. </td>
 +
                <td> &nbsp;<a href="/Team:UCL">Team wiki!</a> </td>
 +
                <td> &nbsp;<a href="www.goodbyeazodye.org.uk">See us here also!</a> </td>
 +
            </tr>
 +
        </tbody>
 +
    </table>
 +
    </font>
</div>
</div>
<!--- This is the third biobrick --->
<!--- This is the third biobrick --->
-
<div id="view4"><div class="textTitle"><h4>Stage 04: Diagnostic digest of azo-reductase plasmids</h4></div><br>
+
<div id="view4"><div class="textTitle"><h4>Special Awards</h4></div><br>
-
<a data-tip="true" class="top large" data-tip-content="This diagram explains the basic construct of the BioBrick, the only part that changes is the selected function itself; in this case attributed to BBa_K1336003." href="javascript:void(0)" style="width: 30%;float: right;margin-left:2%"><img src="https://static.igem.org/mediawiki/2014/7/78/UCLBBLigningperoxidase.png" style="max-width: 100%;"></a>
+
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<p>Usually found in white-rot fungi species, its main function in nature is to participate in lignin-degrading processes by these organisms. However, it has also been found to play a role in <a data-tip="true" class="top large" data-tip-content="Using oxidative processes." href="javascript:void(0)"><b>azo dye degradation and decolourisation</b></a>. <br><br>This enzyme, like laccase, would be incorporated in the second step of the reaction to oxidise the products of the azo bond cleavage, in order to achieve greater detoxification. The sequence for the enzyme will be ordered and synthesised, including the BioBrick prefix and suffix. Again, it will function together with a promoter and a RBS.</p><br>
+
<p>EDO WRITE THINGS HERE</p><br>
 +
    <font size="2">
 +
    <table width="100%" height="auto">
 +
        <thead>
 +
            <tr>
 +
                <th>  </th>
 +
                <th> Task </th>
 +
                <th> Where am I on wiki? </th>
 +
                <th> Where am I on iGEM? </th>
 +
            </tr>
 +
        </thead>
 +
        <tbody>
 +
            <!--Lisbon plasmids-->
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Best Supporting Art and Design </td>
 +
                <td> &nbsp;<a href="/Team:UCL/Humans/Team">Meet our Team!</a> </td>
 +
                <td> &nbsp;<a href="https://igem.org/Team.cgi?id=1336">Team Roster!</a> </td>
 +
            </tr>
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Best Policy and Practice Advance </td>
 +
                <td> &nbsp;<a href="/Team:UCL/Project/Achievements">Our Achievements!</a> </td>
 +
                <td> &nbsp;<a href="https://igem.org/2014_Judging_Form?id=1336">iGEM Judging form!</a> </td>
 +
            </tr>
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Best Supporting Software </td>
 +
                <td> &nbsp;<a href="/Team:UCL">Team wiki!</a> </td>
 +
                <td> &nbsp;<a href="www.goodbyeazodye.org.uk">See us here also!</a> </td>
 +
            </tr>
 +
        </tbody>
 +
    </table>
 +
    </font>
 +
   
 +
    <div>
 +
        <div><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="50px">
 +
        <ul>
 +
        <br>Bronze Medal<br><ul>
 +
            <li> We <a href="https://igem.org/Team.cgi?id=1336">registered</a> our team!</li></br>
 +
            <li> We have completed the <a href="https://igem.org/2014_Judging_Form?id=1336">Judging form!</a></li><br>
 +
            <li> Our team <a href="/Team:UCL">wiki</a> is here!</li><br>
 +
            <li> We have prepared a Poster and a Talk for the iGEM Giant Jamboree!</li><br>
 +
            <li> Our team <a href="/Team:UCL/Humans/Team">attributions</a> can be found here, with our <a href="/Team:UCL/Humans/Collab">external collaborators</a>, and <a href="/Team:UCL/Humans/Sponsor">sponsors</a></li><br>
 +
            <li> Our new <a href="/Team:UCL/Project/Biobrick">BioBrick Parts and Devices</a> have been assembled, characterised, and documented!</li>
 +
        </ul>
 +
        </div>
 +
        <div><img src="https://static.igem.org/mediawiki/2014/e/e8/UCL_Silver-metal-star.jpg" width="50px">
 +
            <ul>
 +
            <br>Silver Medal<br><ul>
 +
                <li> Our new BioBrick Parts and Devices have been <a href="/Team:UCL/Science/Results">experimentally validated</a>, and work as expected!</li></br>
 +
                <li> We documented the characterisation of these parts in the iGEM Parts Registry: <a href="http://parts.igem.org/Part:BBa_K1336003">BBa_1366003</a>, <a href="http://parts.igem.org/Part:BBa_K1336005">BBa_1366005</a>, <a href="http://parts.igem.org/Part:BBa_K1336006">BBa_1366006</a>, <a href="http://parts.igem.org/Part:BBa_K1336007">BBa_1366007</a>.</li><br>
 +
                <li> We submitted those new parts to the iGEM Parts Registry.</li><br>
 +
                <li> We discussed how our project could have implications for the environment, security, safety, ethics, ownership and data sharing. Find out more about this in our <a href="/Team:UCL/Humans/Soci">Sociological Imaginations</a> page!</li>
 +
            </ul>
 +
        </div>
 +
        <div><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="50px">
 +
            <ul>
 +
            <br>Gold Medal<br><ul>
 +
                <li> We <b>improved</b> the function and characterisation of existing <a href="/Team:UCL/Science/Experiment">BioBrick Parts</a>, and entered this new information into the Registry. However, this has not been submitted to the registry.</li></br>
 +
                <li> We <a href="/Team:UCL/Humans/Collab">collaborated</a> with other registered iGEM teams.</li><br>
 +
                <li> We outlined and detailed a new approach to tack the issues of <a href="/Team:UCL/Project/Xenobiology">biosafety</a> in the field of Synthetic Biology. We also explained our <https://2014.igem.org/Team:UCL/Humans/Story>story</a>...</li><br>
 +
            </ul>
 +
        </div>
 +
    </div>
</div>
</div>
-
<!--- This is the fourth biobrick --->
 
-
<div id="view5"><div class="textTitle"><h4>Stage 05: Creation of azo-reductase BioBrick parts from plasmids</h4></div><br>
 
-
<a data-tip="true" class="top large" data-tip-content="This diagram explains the basic construct of the BioBrick, the only part that changes is the selected function itself; in this case attributed to BBa_K1336004." href="javascript:void(0)" style="width: 30%;float: right;margin-left:2%"><img src="https://static.igem.org/mediawiki/2014/a/ad/UCLBBBsdyp.png" style="max-width: 100%;"></a>
 
-
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-
<p>Found in <em>B. subtilis</em>, the physiological function of this newly discovered enzyme is still unclear, although it has shown effectiveness in degrading lignin and azo dyes, which makes it useful for us. It is not as effective as PpDyP for most compounds, but very efficient in degrading ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)). <br><br>The BioBrick will be constructed via PCR.</p><br><br><br><br>
 
-
</div>
 
-
<!--- This is the fifth biobrick --->
 
-
<div id="view6"><div class="textTitle"><h4>Stage 06: Diagnostic digest of azo-reductase BioBrick parts</h4></div><br>
 
-
<a data-tip="true" class="top large" data-tip-content="This diagram explains the basic construct of the BioBrick, the only part that changes is the selected function itself; in this case attributed to BBa_K1336005." href="javascript:void(0)" style="width: 30%;float: right;margin-left:2%"><img src="https://static.igem.org/mediawiki/2014/9/9c/UCLBBPpdyp.png" style="max-width: 100%;"></a>
 
-
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-
<p>This enzyme is found in <em>P. putida</em>. Although it is relatively novel, and has not yet been studied in detail, it seem to be an extremely versatile and powerful biocatalyst; it oxidises a wide <a data-tip="true" class="top large" data-tip-content="Such as azo dyes, anthraquinones, phenolic compounds, manganese or veratryl alcohol." href="javascript:void(0)"><b>variety of substrates</b></a> very efficiently. This will broaden the <a data-tip="true" class="top large" data-tip-content="Going further just azo dyes!" href="javascript:void(0)"><b>spectrum of action</b></a> of our decolourising device, and thus being able to degrade other toxic compounds typically found in industrial wastewaters. <br><br>This BioBrick will be constructed via PCR.</p><br>
 
-
</div>
 
-
 
-
<!--- This is the fifth biobrick --->
 
-
<div id="view7"><div class="textTitle"><h4>Stage 07: Assembling azo-reductase BioBrick Device(s)</h4></div><br>
 
-
<a data-tip="true" class="top large" data-tip-content="This diagram explains the basic construct of the BioBrick, the only part that changes is the selected function itself; in this case attributed to BBa_K1336005." href="javascript:void(0)" style="width: 30%;float: right;margin-left:2%"><img src="https://static.igem.org/mediawiki/2014/9/9c/UCLBBPpdyp.png" style="max-width: 100%;"></a>
 
-
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-
<p>This enzyme is found in <em>P. putida</em>. Although it is relatively novel, and has not yet been studied in detail, it seem to be an extremely versatile and powerful biocatalyst; it oxidises a wide <a data-tip="true" class="top large" data-tip-content="Such as azo dyes, anthraquinones, phenolic compounds, manganese or veratryl alcohol." href="javascript:void(0)"><b>variety of substrates</b></a> very efficiently. This will broaden the <a data-tip="true" class="top large" data-tip-content="Going further just azo dyes!" href="javascript:void(0)"><b>spectrum of action</b></a> of our decolourising device, and thus being able to degrade other toxic compounds typically found in industrial wastewaters. <br><br>This BioBrick will be constructed via PCR.</p><br>
 
-
</div>
 
-
 
-
<!--- This is the fifth biobrick --->
 
-
<div id="view8"><div class="textTitle"><h4>Stage 08: Characterisation of azo-reductase BioBrick devices</h4></div><br>
 
-
<a data-tip="true" class="top large" data-tip-content="This diagram explains the basic construct of the BioBrick, the only part that changes is the selected function itself; in this case attributed to BBa_K1336005." href="javascript:void(0)" style="width: 30%;float: right;margin-left:2%"><img src="https://static.igem.org/mediawiki/2014/9/9c/UCLBBPpdyp.png" style="max-width: 100%;"></a>
 
-
<!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately-->
 
-
<p>This enzyme is found in <em>P. putida</em>. Although it is relatively novel, and has not yet been studied in detail, it seem to be an extremely versatile and powerful biocatalyst; it oxidises a wide <a data-tip="true" class="top large" data-tip-content="Such as azo dyes, anthraquinones, phenolic compounds, manganese or veratryl alcohol." href="javascript:void(0)"><b>variety of substrates</b></a> very efficiently. This will broaden the <a data-tip="true" class="top large" data-tip-content="Going further just azo dyes!" href="javascript:void(0)"><b>spectrum of action</b></a> of our decolourising device, and thus being able to degrade other toxic compounds typically found in industrial wastewaters. <br><br>This BioBrick will be constructed via PCR.</p><br>
 
</div>
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{{:Team:UCL/Template:footerx}}
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Latest revision as of 21:03, 17 October 2014

Goodbye Azodye UCL iGEM 2014

jQuery UI Accordion - Default functionality
Achievements

Bronze Achieved!


EDO WRITE THINGS HERE


Task Where am I on wiki? Where am I on iGEM?
 Team registration  Meet our Team!  Team Roster!
 Complete Judging form  Our Achievements!  iGEM Judging form!
 Team Wiki  Team wiki!  See us here also!
 We will present our project poster and talk at the iGEM Giant Jamboree, 30 October - 3 November 2014  ...  ...
 Use of new/improved application of previously existing standard BioBrick Part or Device, and submitted to the iGEM Registry  ...  ...

Silver Achieved!


EDO WRITE THINGS HERE


Task Where am I on wiki? Where am I on iGEM?
 Experimental validation that at least one of our new Biobrick Part(s) or Device(s) works as expected.  Meet our Team!  Team Roster!
 Characterisation of working Biobrick(s) documented in the "Main Page" section of its Part's/Device's Registry Entry.  Our Achievements!  iGEM Judging form!
 New part submitted to iGEM Parts Registry (adhering to the iGEM Registry guidelines).  Team wiki!  See us here also!
 Implications of our project to the environment, security, safety and ethics, and/or ownership and sharing. Also, description of one or more ways these, or broader, implications have been taken into consideration in the design and execution of our project.  ...  ...

Gold Achieved!


EDO WRITE THINGS HERE


Task Where am I on wiki? Where am I on iGEM?
 Improve the function OR characterization of an existing BioBrick Part or Device, document this in the "Experience" section of its entry in the iGEM Registry, and submit the part to the iGEM Parts Registry.  Meet our Team!  Team Roster!
 Help any registered iGEM team from another school or institute, e.g. characterising a part, debugging a construct, or modelling/simulating their system.  Our Achievements!  iGEM Judging form!
 Describe a novel approach of our team to help us and others consider the implications/aspects of the design and outcomes of synthetic biology efforts; justifying its novelty and how this approach might be adapted and scaled for others to use.  Team wiki!  See us here also!

Special Awards


EDO WRITE THINGS HERE


Task Where am I on wiki? Where am I on iGEM?
 Best Supporting Art and Design  Meet our Team!  Team Roster!
 Best Policy and Practice Advance  Our Achievements!  iGEM Judging form!
 Best Supporting Software  Team wiki!  See us here also!

    Silver Medal
    • Our new BioBrick Parts and Devices have been experimentally validated, and work as expected!

    • We documented the characterisation of these parts in the iGEM Parts Registry: BBa_1366003, BBa_1366005, BBa_1366006, BBa_1366007.

    • We submitted those new parts to the iGEM Parts Registry.

    • We discussed how our project could have implications for the environment, security, safety, ethics, ownership and data sharing. Find out more about this in our Sociological Imaginations page!

    Gold Medal
    • We improved the function and characterisation of existing BioBrick Parts, and entered this new information into the Registry. However, this has not been submitted to the registry.

    • We collaborated with other registered iGEM teams.

    • We outlined and detailed a new approach to tack the issues of biosafety in the field of Synthetic Biology. We also explained our story...

Contact Us

University College London
Gower Street - London
WC1E 6BT
Biochemical Engineering Department
Phone: +44 (0)20 7679 2000
Email: ucligem2014@gmail.com

Follow Us

Retrieved from "http://2014.igem.org/Team:UCL/Tests"