Team:UCL/Tests

From 2014.igem.org

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   <img src="https://static.igem.org/mediawiki/2014/2/21/OExperiments_Bannero.jpg" width="100%" height="100%" alt="Experiments" />
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   <img src="https://static.igem.org/mediawiki/2014/0/07/OAchievements_Bannero.jpg" width="100%" height="100%" alt="Achievements" />
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    <!--DELETE LATER, YKH-->
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<div class="textArena">
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     <h1>Editing, please leave as is.</h1>
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<!--- This is the coding for the tabs (ask sanjay before altering this) --->
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     <!--STOP-->
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<ul class="tabs">
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     <li><a href="#view1">Bronze</a></li>
 +
     <li><a href="#view2">Silver</a></li>
 +
    <li><a href="#view3">Gold</a></li>
 +
    <li><a href="#view4">Special Prizes</a></li>
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</ul>
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<div class="tabcontents">
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    <div>
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<!--- This is the overview section --->
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        <div class="textTitle"><h3 class="widthCorrect">List of Experimental Stages</h3></div>
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<div id="view1"><div class="textTitle"><h4>Bronze Achieved!</h4></div><br>
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        <ol>
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<!-- <a data-tip="true" class="top large" data-tip-content="Here's Tanel doing some pipetting in our lab!" href="javascript:void(0)" style="width: 25%;float: right;margin-left:2%"><img src="https://static.igem.org/mediawiki/2014/c/c9/UCLTANELPIPETTING.JPG" style="max-width: 100%;"></a> -->
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            <li><a href="/Team:UCL/Science/Experiment#Expt01">Stage 01: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit</a></li>
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<!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately-->
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             <li><a href="/Team:UCL/Science/Experiment#Expt02">Stage 02: Identification of useful genes for making new BioBricks</a></li>
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<p>EDO WRITE THINGS HERE</p><br>
-
            <li><a href="/Team:UCL/Science/Experiment#Expt03">Stage 03: Transforming <i>E. coli</i> with azo-reductase plasmids</a></li>
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    <font size="2">
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            <li><a href="/Team:UCL/Science/Experiment#Expt04">Stage 04: Diagnostic digest of azo-reductase plasmids</a></li>
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    <table width="100%" height="auto">
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            <li><a href="/Team:UCL/Science/Experiment#Expt05">Stage 05: Creation of azo-reductase BioBrick parts from plasmids</a></li>
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        <thead>
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             <li><a href="/Team:UCL/Science/Experiment#Expt06">Stage 06: Diagnostic digest of azo-reductase BioBrick parts</a></li>
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            <tr>
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             <li><a href="/Team:UCL/Science/Experiment#Expt07">Stage 07: Assembling azo-reductase BioBrick Device(s)</a></li>
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                <th>  </th>
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             <li><a href="/Team:UCL/Science/Experiment#Expt08">Stage 08: Characterisation of azo-reductase BioBrick devices</a></li>
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                <th> Task </th>
-
        </ol></p>
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                <th> Where am I on wiki? </th>
-
         <hr>
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                <th> Where am I on iGEM? </th>
-
     </div>
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            </tr>
 +
        </thead>
 +
        <tbody>
 +
             <!--Lisbon plasmids-->
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Team registration </td>
 +
                <td> &nbsp;<a href="/Team:UCL/Humans/Team">Meet our Team!</a> </td>
 +
                <td> &nbsp;<a href="https://igem.org/Team.cgi?id=1336">Team Roster!</a> </td>
 +
            </tr>
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Complete Judging form </td>
 +
                <td> &nbsp;<a href="/Team:UCL/Project/Achievements">Our Achievements!</a> </td>
 +
                <td> &nbsp;<a href="https://igem.org/2014_Judging_Form?id=1336">iGEM Judging form!</a> </td>
 +
             </tr>
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Team Wiki </td>
 +
                <td> &nbsp;<a href="/Team:UCL">Team wiki!</a> </td>
 +
                <td> &nbsp;<a href="www.goodbyeazodye.org.uk">See us here also!</a> </td>
 +
             </tr>
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;We will present our project poster and talk at the iGEM Giant Jamboree, 30 October - 3 November 2014 </td>
 +
                <td> &nbsp;<a href="/Team:UCL">...</a> </td>
 +
                <td> &nbsp;<a href=" ">...</a> </td>
 +
             </tr>
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Use of new/improved application of previously existing standard BioBrick Part or Device, and submitted to the iGEM Registry </td>
 +
                <td> &nbsp;<a href="/Team:UCL/Project/Biobricks">...</a> </td>
 +
                <td> &nbsp;<a href=" ">...</a> </td>
 +
            </tr>
 +
         </tbody>
 +
     </table>
 +
    </font>
 +
</div>
-
    <div>
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<!--- This is the first biobrick --->
-
         <h2>Experiments</h2>             
+
<div id="view2"><div class="textTitle"><h4>Silver Achieved!</h4></div><br>
 +
<!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately-->
 +
<p>EDO WRITE THINGS HERE</p><br>
 +
    <font size="2">
 +
    <table width="100%" height="auto">
 +
         <thead>
 +
            <tr>
 +
                <th>  </th>
 +
                <th> Task </th>
 +
                <th> Where am I on wiki? </th>
 +
                <th> Where am I on iGEM? </th>
 +
             </tr>
 +
        </thead>
 +
        <tbody>
 +
            <!--Lisbon plasmids-->
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e8/UCL_Silver-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Experimental validation that at least one of our new Biobrick Part(s) or Device(s) works as expected. </td>
 +
                <td> &nbsp;<a href="/Team:UCL/Humans/Team">Meet our Team!</a> </td>
 +
                <td> &nbsp;<a href="https://igem.org/Team.cgi?id=1336">Team Roster!</a> </td>
 +
            </tr>
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e8/UCL_Silver-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Characterisation of working Biobrick(s) documented in the "Main Page" section of its Part's/Device's Registry Entry. </td>
 +
                <td> &nbsp;<a href="/Team:UCL/Project/Achievements">Our Achievements!</a> </td>
 +
                <td> &nbsp;<a href="https://igem.org/2014_Judging_Form?id=1336">iGEM Judging form!</a> </td>
 +
            </tr>
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e8/UCL_Silver-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;New part submitted to iGEM Parts Registry (adhering to the iGEM Registry guidelines). </td>
 +
                <td> &nbsp;<a href="/Team:UCL">Team wiki!</a> </td>
 +
                <td> &nbsp;<a href="www.goodbyeazodye.org.uk">See us here also!</a> </td>
 +
            </tr>
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/e/e8/UCL_Silver-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Implications of our project to the environment, security, safety and ethics, and/or ownership and sharing. Also, description of one or more ways these, or broader, implications have been taken into consideration in the design and execution of our project. </td>
 +
                <td> &nbsp;<a href="/Team:UCL">...</a> </td>
 +
                <td> &nbsp;<a href=" ">...</a> </td>
 +
            </tr>
 +
        </tbody>
 +
    </table>
 +
    </font>
 +
</div>
-
<!--STAGE 01-->
+
<!--- This is the second biobrick --->
-
        <div>
+
<div id="view3"><div class="textTitle"><h4>Gold Achieved!</h4></div><br>
-
            <h4><a name="Expt01">Stage 01: Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit</a></h4>
+
<!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately-->
-
            <strong>Protocols&nbsp;&nbsp;</strong>
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<p>EDO WRITE THINGS HERE</p><br>
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
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    <font size="2">
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a>
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    <table width="100%" height="auto">
-
            <br>
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        <thead>
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             <p>[Insert table of useful Distribution BioBricks]</p>
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            <tr>
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             <br>
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                <th>  </th>
-
 
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                <th> Task </th>
-
             <div class="accordion">
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                <th> Where am I on wiki? </th>
-
                 <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
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                <th> Where am I on iGEM? </th>
-
                    <div>
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             </tr>
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                        <p>...</p>
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        </thead>
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                    </div>
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        <tbody>
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                 <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
+
             <!--Lisbon plasmids-->
-
                    <div>
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             <tr>
-
                        <p>...</p>
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                 <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
-
                    </div>
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                <td> &nbsp;Improve the function OR characterization of an existing BioBrick Part or Device, document this in the "Experience" section of its entry in the iGEM Registry, and submit the part to the iGEM Parts Registry. </td>
-
             </div>
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                <td> &nbsp;<a href="/Team:UCL/Humans/Team">Meet our Team!</a> </td>
-
            <br>
+
                <td> &nbsp;<a href="https://igem.org/Team.cgi?id=1336">Team Roster!</a> </td>
 +
            </tr>
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Help any registered iGEM team from another school or institute, e.g. characterising a part, debugging a construct, or modelling/simulating their system. </td>
 +
                 <td> &nbsp;<a href="/Team:UCL/Project/Achievements">Our Achievements!</a> </td>
 +
                <td> &nbsp;<a href="https://igem.org/2014_Judging_Form?id=1336">iGEM Judging form!</a> </td>
 +
            </tr>
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Describe a novel approach of our team to help us and others consider the implications/aspects of the design and outcomes of synthetic biology efforts; justifying its novelty and how this approach might be adapted and scaled for others to use. </td>
 +
                <td> &nbsp;<a href="/Team:UCL">Team wiki!</a> </td>
 +
                <td> &nbsp;<a href="www.goodbyeazodye.org.uk">See us here also!</a> </td>
 +
             </tr>
 +
        </tbody>
 +
    </table>
 +
    </font>
 +
</div>
 +
<!--- This is the third biobrick --->
 +
<div id="view4"><div class="textTitle"><h4>Special Awards</h4></div><br>
 +
<!-- This is the main text. Anything in a <p>TEXT</p> is a paragraph and will be spaced appropriately-->
 +
<p>EDO WRITE THINGS HERE</p><br>
 +
    <font size="2">
 +
    <table width="100%" height="auto">
 +
        <thead>
 +
            <tr>
 +
                <th>  </th>
 +
                <th> Task </th>
 +
                <th> Where am I on wiki? </th>
 +
                <th> Where am I on iGEM? </th>
 +
            </tr>
 +
        </thead>
 +
        <tbody>
 +
            <!--Lisbon plasmids-->
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Best Supporting Art and Design </td>
 +
                <td> &nbsp;<a href="/Team:UCL/Humans/Team">Meet our Team!</a> </td>
 +
                <td> &nbsp;<a href="https://igem.org/Team.cgi?id=1336">Team Roster!</a> </td>
 +
            </tr>
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Best Policy and Practice Advance </td>
 +
                <td> &nbsp;<a href="/Team:UCL/Project/Achievements">Our Achievements!</a> </td>
 +
                <td> &nbsp;<a href="https://igem.org/2014_Judging_Form?id=1336">iGEM Judging form!</a> </td>
 +
            </tr>
 +
            <tr>
 +
                <td> <center><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="25px"></center> </td>
 +
                <td> &nbsp;Best Supporting Software </td>
 +
                <td> &nbsp;<a href="/Team:UCL">Team wiki!</a> </td>
 +
                <td> &nbsp;<a href="www.goodbyeazodye.org.uk">See us here also!</a> </td>
 +
            </tr>
 +
        </tbody>
 +
    </table>
 +
    </font>
 +
   
 +
    <div>
 +
        <div><img src="https://static.igem.org/mediawiki/2014/e/e0/UCL_Bronze-metal-star.jpg" width="50px">
 +
        <ul>
 +
        <br>Bronze Medal<br><ul>
 +
            <li> We <a href="https://igem.org/Team.cgi?id=1336">registered</a> our team!</li></br>
 +
            <li> We have completed the <a href="https://igem.org/2014_Judging_Form?id=1336">Judging form!</a></li><br>
 +
            <li> Our team <a href="/Team:UCL">wiki</a> is here!</li><br>
 +
            <li> We have prepared a Poster and a Talk for the iGEM Giant Jamboree!</li><br>
 +
            <li> Our team <a href="/Team:UCL/Humans/Team">attributions</a> can be found here, with our <a href="/Team:UCL/Humans/Collab">external collaborators</a>, and <a href="/Team:UCL/Humans/Sponsor">sponsors</a></li><br>
 +
            <li> Our new <a href="/Team:UCL/Project/Biobrick">BioBrick Parts and Devices</a> have been assembled, characterised, and documented!</li>
 +
        </ul>
         </div>
         </div>
-
 
+
         <div><img src="https://static.igem.org/mediawiki/2014/e/e8/UCL_Silver-metal-star.jpg" width="50px">
-
<!--STAGE 02-->
+
            <ul>
-
         <div>
+
             <br>Silver Medal<br><ul>
-
            <h4><a name="Expt02">Stage 02: Identification of useful genes for making new BioBricks</a></h4>
+
                <li> Our new BioBrick Parts and Devices have been <a href="/Team:UCL/Science/Results">experimentally validated</a>, and work as expected!</li></br>
-
            <strong>Protocols&nbsp;&nbsp;</strong>
+
                <li> We documented the characterisation of these parts in the iGEM Parts Registry: <a href="http://parts.igem.org/Part:BBa_K1336003">BBa_1366003</a>, <a href="http://parts.igem.org/Part:BBa_K1336005">BBa_1366005</a>, <a href="http://parts.igem.org/Part:BBa_K1336006">BBa_1366006</a>, <a href="http://parts.igem.org/Part:BBa_K1336007">BBa_1366007</a>.</li><br>
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">PCR</span></a>
+
                <li> We submitted those new parts to the iGEM Parts Registry.</li><br>
-
             <a href="/Team:UCL/Science/Proto"><span class="label label-warning">analytical digest</span></a>
+
                 <li> We discussed how our project could have implications for the environment, security, safety, ethics, ownership and data sharing. Find out more about this in our <a href="/Team:UCL/Humans/Soci">Sociological Imaginations</a> page!</li>
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">gel</span></a>
+
             </ul>
-
            (<a href="/Team:UCL/Science/Proto"><span class="label label-warning">digest</span></a>
+
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">ligation</span></a>
+
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
+
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
+
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a>)
+
-
            <br>
+
-
            <p>[Insert table of Our Genes]</p>
+
-
            <br>
+
-
            <div class="accordion">
+
-
                 <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
+
-
                    <div>
+
-
                        <p>...</p>
+
-
                    </div>
+
-
                <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
+
-
                    <div>
+
-
                        <p>...</p>
+
-
                    </div>
+
-
             </div>
+
-
            <br>
+
         </div>
         </div>
-
 
+
         <div><img src="https://static.igem.org/mediawiki/2014/3/36/UCL_Gold-metal-star.jpg" width="50px">
-
<!--STAGE 03-->
+
            <ul>
-
         <div>
+
             <br>Gold Medal<br><ul>
-
            <h4><a name="Expt03">Stage 03: Transforming <i>E. coli</i> with azo-reductase plasmids</a></h4>
+
                <li> We <b>improved</b> the function and characterisation of existing <a href="/Team:UCL/Science/Experiment">BioBrick Parts</a>, and entered this new information into the Registry. However, this has not been submitted to the registry.</li></br>
-
            <strong>Protocols&nbsp;&nbsp;</strong>
+
                <li> We <a href="/Team:UCL/Humans/Collab">collaborated</a> with other registered iGEM teams.</li><br>
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">PCR</span></a>
+
                <li> We outlined and detailed a new approach to tack the issues of <a href="/Team:UCL/Project/Xenobiology">biosafety</a> in the field of Synthetic Biology. We also explained our <https://2014.igem.org/Team:UCL/Humans/Story>story</a>...</li><br>
-
             <a href="/Team:UCL/Science/Proto"><span class="label label-warning">analytical digest</span></a>
+
             </ul>
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">gel</span></a>
+
-
            (<a href="/Team:UCL/Science/Proto"><span class="label label-warning">digest</span></a>
+
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">ligation</span></a>
+
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
+
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
+
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a>)
+
-
            <br>
+
-
            <p>...</p>
+
-
            <br>
+
-
            <div class="accordion">
+
-
                <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
+
-
                    <div>
+
-
                        <p>...</p>
+
-
                    </div>
+
-
                <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
+
-
                    <div>
+
-
                        <p>...</p>
+
-
                    </div>
+
-
             </div>
+
-
            <br>
+
         </div>
         </div>
-
 
-
<!--STAGE 04-->
 
-
        <div>
 
-
            <h4><a name="Expt04">Stage 04: Diagnostic digest of azo-reductase plasmids</a></h4>
 
-
            <strong>Protocols&nbsp;&nbsp;</strong>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">PCR</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">analytical digest</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">gel</span></a>
 
-
            (<a href="/Team:UCL/Science/Proto"><span class="label label-warning">digest</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">ligation</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a>)
 
-
            <br>
 
-
            <p>...</p>
 
-
            <br>
 
-
            <div class="accordion">
 
-
                <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
 
-
                    <div>
 
-
                        <p>...</p>
 
-
                    </div>
 
-
                <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
 
-
                    <div>
 
-
                        <p>...</p>
 
-
                    </div>
 
-
            </div>
 
-
            <br>
 
-
        </div>
 
-
 
-
<!--STAGE 05-->
 
-
        <div>
 
-
            <h4><a name="Expt05">Stage 05: Creation of azo-reductase BioBrick parts from plasmids</a></h4>
 
-
            <strong>Protocols&nbsp;&nbsp;</strong>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">PCR</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">analytical digest</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">gel</span></a>
 
-
            (<a href="/Team:UCL/Science/Proto"><span class="label label-warning">digest</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">ligation</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a>)
 
-
            <br>
 
-
            <p>...</p>
 
-
            <br>
 
-
            <div class="accordion">
 
-
                <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
 
-
                    <div>
 
-
                        <p>...</p>
 
-
                    </div>
 
-
                <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
 
-
                    <div>
 
-
                        <p>...</p>
 
-
                    </div>
 
-
            </div>
 
-
            <br>
 
-
        </div>
 
-
 
-
<!--STAGE 06-->
 
-
        <div>
 
-
            <h4><a name="Expt06">Stage 06: Diagnostic digest of azo-reductase BioBrick parts</a></h4>
 
-
            <strong>Protocols&nbsp;&nbsp;</strong>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">PCR</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">analytical digest</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">gel</span></a>
 
-
            (<a href="/Team:UCL/Science/Proto"><span class="label label-warning">digest</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">ligation</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a>)
 
-
            <br>
 
-
            <p>...</p>
 
-
            <br>
 
-
            <div class="accordion">
 
-
                <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
 
-
                    <div>
 
-
                        <p>...</p>
 
-
                    </div>
 
-
                <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
 
-
                    <div>
 
-
                        <p>...</p>
 
-
                    </div>
 
-
            </div>
 
-
            <br>
 
-
        </div>
 
-
 
-
<!--STAGE 07-->
 
-
        <div>
 
-
            <h4><a name="Expt07">Stage 07: Assembling azo-reductase BioBrick Device(s)</a></h4>
 
-
            <strong>Protocols&nbsp;&nbsp;</strong>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">PCR</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">analytical digest</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">gel</span></a>
 
-
            (<a href="/Team:UCL/Science/Proto"><span class="label label-warning">digest</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">ligation</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a>)
 
-
            <br>
 
-
            <p>...</p>
 
-
            <br>
 
-
            <div class="accordion">
 
-
                <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
 
-
                    <div>
 
-
                        <p>...</p>
 
-
                    </div>
 
-
                <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
 
-
                    <div>
 
-
                        <p>...</p>
 
-
                    </div>
 
-
            </div>
 
-
            <br>
 
-
        </div>
 
-
 
-
<!--STAGE 08-->
 
-
        <div>
 
-
            <h4><a name="Expt08">Stage 08: Characterisation of azo-reductase BioBrick devices</a></h4>
 
-
            <strong>Protocols&nbsp;&nbsp;</strong>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">PCR</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">analytical digest</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">gel</span></a>
 
-
            (<a href="/Team:UCL/Science/Proto"><span class="label label-warning">digest</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">ligation</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
 
-
            <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a>)
 
-
            <br>
 
-
            <p>...</p>
 
-
            <br>
 
-
            <div class="accordion">
 
-
                <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
 
-
                    <div>
 
-
                        <p>...</p>
 
-
                    </div>
 
-
                <h4><div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr></div></h4>
 
-
                    <div>
 
-
                        <p>...</p>
 
-
                    </div>
 
-
            </div>
 
-
            <br>
 
-
        </div>
 
-
 
     </div>
     </div>
 +
</div>
-
    <hr></hr>
 
-
<!--PAST HTML. TO COPY, then DELETE.-->
+
</div>
-
    <h4><a name="Expt00">Extraction of <i>Bacillus subtilis</i> genomic DNA</a></h4>
+
-
    <div class="byline"><i class="icon-user"></i> Adam Denyer, Tanel Ozdemir &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="June 13, 2014">June 13, 2014</abbr>
+
-
    <strong>&nbsp;&nbsp;Protocols&nbsp;&nbsp;</strong>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">DNA extraction</span></a></div>
+
-
    <br/>
+
-
    <p>Our literature search identified a number of bacterial species that have been proven to degrade azo dye compounds including <i>B. subtilis</i> and <i>P. aeruginosa</i>.  We were able to obtain a <i>B. subtilis</i> strain for use in our project from ?.  We extracted the genomic DNA from this strain using a Promega Wizard Genomic DNA extraction kit so that we could subsequently amplify the azo-reducatase gene (AzoR1) and create our first azo-reductase BioBrick.  After completing the genomic DNA extracton we ran a gel to show that we had successfully extracted the <i>B. subtilis</i> genomic DNA.</p>
+
-
                   
+
-
    <h4>Transforming <i>E. coli</i> with Azo-reductase plasmids</h4>
+
-
    <div class="byline"><i class="icon-user"></i> Adam Denyer &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013,  8:21 PM">October 15, 2013</abbr>
+
-
    <strong>&nbsp;&nbsp;Protocols&nbsp;&nbsp;</strong>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">PCR</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a></div>
+
-
    <br/>
+
-
    <p>We were gratefully provided with a set of five plasmids from a group of researchers working at the University of Lisbon, Portugal who are researching how azo-dye degrading enzymes function and who were keen to collaborate with us.  These plasmids contained a number of genes encoding azo-dye degrading enzymes from both <i>B. subtilis</i> and <i>P. putida</i>  including mutated forms found to exhibit enhanced degradation activity.  As the DNA concentration of the plasmids we were sent was insufficient to perform PCR amplification on we transformed each of these plasmids into our <i>E. coli</i> NEB5alpha competent cells.  After growing the cells overnight we then mini-prepped each of them to obtain plasmids at sufficient concentrations for future experimental work.</p>
+
-
 
+
-
    <table class="table table-striped table-bordered">
+
-
      <thead>
+
-
        <tr>
+
-
          <th> Name </th>
+
-
          <th> Function </th>
+
-
          <th> Source </th>
+
-
          <th> Concentration </th>
+
-
          <th> Sequence </th>
+
-
          <th> Initial Plasmid / Vector </th>
+
-
          <th> Comments </th>
+
-
        </tr>
+
-
      </thead>
+
-
      <tbody>
+
-
 
+
-
        <tr>
+
-
          <td> pAzoR </td>
+
-
          <td> FMN-dependent NADH-azoreductase 1 </td>
+
-
          <td> <em>Pseudomonas putida</em> </td>
+
-
          <td> Miniprep,
+
-
            <br>48 ng/uL,
+
-
          </td>
+
-
          <td><a href=" http://www.ncbi.nlm.nih.gov/nuccore/26986745?report=fasta&from=3267527&to=3268138">597 bp <strong>[Check! Not 612 bp?]</strong></a></td>
+
-
          <td> Expression vector <a href="http://www.addgene.org/browse/sequence_vdb/2549/ ">pET-21a (+) <a href=" http://biochem.web.utah.edu/hill/links/pET21.pdf">(ampicillin resistant (ampR))</a>, initially cloned between <em>NdeI</em> and <em>BamHI</em>.</td>
+
-
          <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/21655981">Plasmid provided by Lisbon</a></td>
+
-
        </tr>
+
-
 
+
-
        <tr>
+
-
          <td> p1B6 (AzoR 1B6) </td>
+
-
          <td> Mutant: Heat-stable; FMN-dependent NADH-azoreductase 1 </td>
+
-
          <td> <em>Pseudomonas putida</em> </td>
+
-
          <td> Miniprep,
+
-
            <br>68 ng/uL,
+
-
          </td>
+
-
          <td><a href="">597 bp <strong>[Check! Not 612 bp?]</strong></strong> </td>
+
-
          <td> Expression vector <a href="http://www.addgene.org/browse/sequence_vdb/2549/ ">pET-21a (+) <a href=" http://biochem.web.utah.edu/hill/links/pET21.pdf">(ampR)</a>, initially cloned between <em>NdeI</em> and <em>BamHI</em>. </td>
+
-
          <td> <a href=" http://www.ncbi.nlm.nih.gov/pubmed/24475252">Plasmid provided by Lisbon</a>.</td>
+
-
        </tr>
+
-
 
+
-
        <tr>
+
-
          <td> pCotA </td>
+
-
          <td> Spore Coat Protein Laccase </td>
+
-
          <td> <em>Bacillus subtilis</em> </td>
+
-
          <td> Miniprep,
+
-
            <br>103 ng/uL
+
-
          </td>
+
-
          <td><a href="">1733 bp <strong>[Check! Not 1539 bp?]</strong></strong> </td>
+
-
          <td> Expression vector <a href="http://www.addgene.org/browse/sequence_vdb/2549/ ">pET-21a (+) <a href=" http://biochem.web.utah.edu/hill/links/pET21.pdf">(ampR)</a>, initially cloned between <em>NheI</em> and <em>BamHI</em>. </td>
+
-
          <td> <a href=" http://www.itqb.unl.pt/martins/index_files/JBC2002.pdf">Plasmid provided by Lisbon</a>. </td>
+
-
        </tr>
+
-
 
+
-
        <tr>
+
-
          <td> pBsDyP </td>
+
-
          <td> Dye Decolourising Peroxidase BSU38260 </td>
+
-
          <td> <em>Bacillus subtilis</em> </td>
+
-
          <td> Miniprep,
+
-
            <br>51 ng/uL,
+
-
          </td>
+
-
          <td><a href="">1251 bp</td>
+
-
          <td> Expression vector <a href="http://www.addgene.org/browse/sequence_vdb/2549/ ">pET-21a (+) <a href=" http://biochem.web.utah.edu/hill/links/pET21.pdf">(ampR)</a>, initially cloned between <em>NdeI</em> and <em>BamHI</em>. </td>
+
-
          <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/23820555">Plasmid provided by Lisbon</a>.</td>
+
-
        </tr>
+
-
 
+
-
        <tr>
+
-
          <td> pPpDyP </td>
+
-
          <td> Dye Decolourising Peroxidase PP_3248 </td>
+
-
          <td> <em>Pseudomonas putida</em> </td>
+
-
          <td> Miniprep,
+
-
            <br>55 ng/uL</td>
+
-
          <td><a href="">861 bp <strong>[Check! Not 864 bp?]</strong></strong> </td>
+
-
          <td> Expression vector <a href="http://www.addgene.org/browse/sequence_vdb/2549/ ">pET-21a (+) <a href=" http://biochem.web.utah.edu/hill/links/pET21.pdf">(ampR)</a>, initially cloned between <em>NdeI</em> and <em>BamHI</em>. </td>
+
-
            <td><a href="  http://www.ncbi.nlm.nih.gov/pubmed/23820555">Plasmid provided by Lisbon</a>.</td>
+
-
          </tr>
+
-
 
+
-
      </tbody>
+
-
    </table> 
+
-
 
+
-
    <h4>Diagnostic digest of azo-reductase plasmids</h4>
+
-
    <div class="byline"><i class="icon-user"></i> Adam Denyer &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013,  8:21 PM">October 15, 2013</abbr>
+
-
    <strong>&nbsp;&nbsp;Protocols&nbsp;&nbsp;</strong>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">digest</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">gel</span></a></div>
+
-
    <br/>
+
-
    <p>After successfully transforming these plasmids into competent <i>E. coli</i> NEB5alpha cells we then performed a diagnostic digest and gel electrophoresis experiment to ascertain that these plasmids contained the gene we expected.  Each plasmid was digested using two restriction enzymes chosen to digest DNA as specific points on the plasmids and create fragments of known length which we could then confirm using gel electrophoresis.</p>
+
-
 
+
-
    <h4>Creation of azo-reductase BioBrick parts from plasmids</h4>
+
-
    <div class="byline"><i class="icon-user"></i> Adam Denyer &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013,  8:21 PM">October 15, 2013</abbr>
+
-
    <strong>&nbsp;&nbsp;Protocols&nbsp;&nbsp;</strong>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a></div>
+
-
    <br/>
+
-
    <p>senectus et netus et malesuada</p>
+
-
 
+
-
    <h4>Diagnostic digest of azo-reductase BioBrick parts</h4>
+
-
    <div class="byline"><i class="icon-user"></i> Adam Denyer &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013,  8:21 PM">October 15, 2013</abbr>
+
-
    <strong>&nbsp;&nbsp;Protocols&nbsp;&nbsp;</strong>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">digest</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">gel</span></a>
+
-
    </div>
+
-
    <br/>
+
-
    <p>senectus et netus et malesuada</p>
+
-
 
+
-
    <h4>Extraction of useful BioBrick plasmids from iGEM 2014 Distribution Kit</h4>
+
-
    <div class="byline"><i class="icon-user"></i> Adam Denyer &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013,  8:21 PM">October 15, 2013</abbr>
+
-
    &nbsp;&nbsp;<strong>Protocols&nbsp;&nbsp;</strong>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">digest</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">gel</span></a></div>
+
-
    <br/>
+
-
    <p>We began our project by identifying a range of BioBrick parts present in the iGEM 2014 distribution kit which we required as part of our cloning strategy.  These parts primarily consisted of both constituitive and inducible promoter systems with ribosome binding sites which we could then use in conjunction with our azo-reductase BioBricks to assemble a functional azo dye degrading gene.  We also decided that we would use the Red Florescent Protein expresing BioBrick as a control for any further transformation experiments.  As the level of DNA present within each plate of the distribution kit is insufficient to perform digest and ligation reactions on it was necessary to transform each of these plasmids into our NEB5alpha competent cells.  After growing our transformed cells overnight we then mini-prepped each of them to obtain BioBrick plasmids at suitable concentrations for future experiments.</p>
+
-
 
+
-
    <h4>Assembling azo-reductase BioBrick Device(s)</h4>
+
-
    <div class="byline"><i class="icon-user"></i> Adam Denyer &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013,  8:21 PM">October 15, 2013</abbr>
+
-
    <strong>&nbsp;&nbsp;Protocols&nbsp;&nbsp;</strong>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">digest</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">gel</span></a></div>
+
-
    <br/>
+
-
    <p>senectus et netus et malesuada</p>
+
-
 
+
-
    <h4>Characterisation of azo-reductase BioBrick devices</h4>
+
-
    <div class="byline"><i class="icon-user"></i> Adam Denyer &nbsp;&nbsp; <i class="icon-time"></i> <abbr class="published" title="Monday, October 15, 2013,  8:21 PM">October 15, 2013</abbr>
+
-
    <strong>Protocols&nbsp;&nbsp;</strong>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">competent cells</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">transformation</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">miniprep</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">digest</span></a>
+
-
    <a href="/Team:UCL/Science/Proto"><span class="label label-warning">gel</span></a></div>
+
-
    <br/>
+
-
    <p>senectus et netus et malesuada</p>
+
 +
</div>
 +
</div>
 +
<a href="#" class="back-to-top"><img src="https://static.igem.org/mediawiki/2014/5/5d/Scroll_Up_Button.png" width="64" height="64"></a>
 +
<script type="text/javascript">           
 +
jQuery(document).ready(function() {
 +
var offset = 220;
 +
var duration = 500;
 +
jQuery(window).scroll(function() {
 +
if (jQuery(this).scrollTop() > offset) {
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jQuery('.back-to-top').fadeIn(duration);
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} else {
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jQuery('.back-to-top').fadeOut(duration);
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}
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jQuery('.back-to-top').click(function(event) {
 +
event.preventDefault();
 +
jQuery('html, body').animate({scrollTop: 0}, duration);
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return false;
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})
 +
});
 +
</script>
 +
</div><!-- This div end tag should end the .textArena tag that defines light grey space. This is crucial-->
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</div><!-- This is the css of the page. Dont change it unless you have consulted with Lewis or Adam about what your changing-->
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<style>
<style>
/*=======PAGE HEADER=======*/
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    font-family: 'Open Sans', 'Helvetica Neue', Helvetica, Arial, sans-serif;
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      text-align: justify;
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table, th, td {
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/*=========Object Alignment Classes=============*/
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margin-left:3%;
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.alignleft {
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float:left;
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margin-right:3%;
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}
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.aligncenter {
 +
float:center;
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margin-right:3%;
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/*=========Table Classes=============*/
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    border: 1px solid black;
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bottom: 0em;
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right: 0.5em;
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background-color: #AA8799;
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text-decoration: none;
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color: #000000;
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display: none;
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border-radius: 100px;
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padding-bottom: 25px;
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margin-bottom: -25px;
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z-index: 99999;
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    display: table; /* 2 */
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</style>
</body>
</body>
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</html>
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{{:Team:UCL/Template:footerx}}
{{:Team:UCL/Template:footerx}}

Latest revision as of 21:03, 17 October 2014

Goodbye Azodye UCL iGEM 2014

jQuery UI Accordion - Default functionality
Achievements

Bronze Achieved!


EDO WRITE THINGS HERE


Task Where am I on wiki? Where am I on iGEM?
 Team registration  Meet our Team!  Team Roster!
 Complete Judging form  Our Achievements!  iGEM Judging form!
 Team Wiki  Team wiki!  See us here also!
 We will present our project poster and talk at the iGEM Giant Jamboree, 30 October - 3 November 2014  ...  ...
 Use of new/improved application of previously existing standard BioBrick Part or Device, and submitted to the iGEM Registry  ...  ...

Silver Achieved!


EDO WRITE THINGS HERE


Task Where am I on wiki? Where am I on iGEM?
 Experimental validation that at least one of our new Biobrick Part(s) or Device(s) works as expected.  Meet our Team!  Team Roster!
 Characterisation of working Biobrick(s) documented in the "Main Page" section of its Part's/Device's Registry Entry.  Our Achievements!  iGEM Judging form!
 New part submitted to iGEM Parts Registry (adhering to the iGEM Registry guidelines).  Team wiki!  See us here also!
 Implications of our project to the environment, security, safety and ethics, and/or ownership and sharing. Also, description of one or more ways these, or broader, implications have been taken into consideration in the design and execution of our project.  ...  ...

Gold Achieved!


EDO WRITE THINGS HERE


Task Where am I on wiki? Where am I on iGEM?
 Improve the function OR characterization of an existing BioBrick Part or Device, document this in the "Experience" section of its entry in the iGEM Registry, and submit the part to the iGEM Parts Registry.  Meet our Team!  Team Roster!
 Help any registered iGEM team from another school or institute, e.g. characterising a part, debugging a construct, or modelling/simulating their system.  Our Achievements!  iGEM Judging form!
 Describe a novel approach of our team to help us and others consider the implications/aspects of the design and outcomes of synthetic biology efforts; justifying its novelty and how this approach might be adapted and scaled for others to use.  Team wiki!  See us here also!

Special Awards


EDO WRITE THINGS HERE


Task Where am I on wiki? Where am I on iGEM?
 Best Supporting Art and Design  Meet our Team!  Team Roster!
 Best Policy and Practice Advance  Our Achievements!  iGEM Judging form!
 Best Supporting Software  Team wiki!  See us here also!

    Silver Medal
    • Our new BioBrick Parts and Devices have been experimentally validated, and work as expected!

    • We documented the characterisation of these parts in the iGEM Parts Registry: BBa_1366003, BBa_1366005, BBa_1366006, BBa_1366007.

    • We submitted those new parts to the iGEM Parts Registry.

    • We discussed how our project could have implications for the environment, security, safety, ethics, ownership and data sharing. Find out more about this in our Sociological Imaginations page!

    Gold Medal
    • We improved the function and characterisation of existing BioBrick Parts, and entered this new information into the Registry. However, this has not been submitted to the registry.

    • We collaborated with other registered iGEM teams.

    • We outlined and detailed a new approach to tack the issues of biosafety in the field of Synthetic Biology. We also explained our story...

Contact Us

University College London
Gower Street - London
WC1E 6BT
Biochemical Engineering Department
Phone: +44 (0)20 7679 2000
Email: ucligem2014@gmail.com

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