Team:UTK-Knoxville

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:UTK-Knoxville&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<h1>Design Construction and Characterization<br/>of Modular Chimeric Biosensors</h1>
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<a href="https://2014.igem.org/Team:UTK-Knoxville/Project"style="color:#000000"> Project</a></td>
 
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Home
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<!-- Links to other team pages -->
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<ul>
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<li><a href="https://2014.igem.org/Team:UTK-Knoxville">Home</a> </li>
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<li><a href="https://2014.igem.org/Team:UTK-Knoxville/Team">Team</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=UTK-Knoxville">Official Team Profile</a> </li>
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<li><a href="https://2014.igem.org/Team:UTK-Knoxville/Project">Project</a> </li>
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<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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<h1>Abstract</h1>
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<img id="RFP" src="https://static.igem.org/mediawiki/2014/f/f6/Rfp-ecoli.png" align="right" />
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<p>&nbsp;&nbsp;A major problem in modern biology is a lack of well understood microbial sensors and one limitation in
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metabolic engineering is an inability to control dynamic cell metabolism. Our project provides a solution
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to both problems with synthetic biology via a synthetic sensing scaffold. There are no standardized
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platforms for sensor characterization today, and as synthetic biology grows, so does the need to develop
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standards for the creation of genetic construct. The completion of this project provides a high throughput
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method of developing a library of microbial sensors, a mechanism for controlling cell metabolism based on
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environmental conditions, and a standardized platform for the design and characterization of synthetic sensing
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circuits.<br><br>
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&nbsp; &nbsp; &nbsp;This project aims to introduce a “plug-and-play” method for sensing circuits, and to provide a protocol for
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characterizing novel sensors. A two-plasmid system, with a sensor and reporter, is employed in our standard.
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The sensor is created by generating a chimera between a sensing gene and a histidine kinase, which activates a
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transcriptional regulation protein. This protein controls the activity of the reporter. This upregulated reporter
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with a fluorescent protein will respond by fluorescing in the presence of our target due to sensor activity.<br><br>
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&nbsp;&nbsp;&nbsp;A wide range of sensing domains can be used and characterized in this manner, making this system highly modular.
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This modularity leads to high throughput sensor characterization, which can be used with both known and unknown sensing domains.
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A successful biosensor circuit platform helps bridge the gap in today’s protocols, modeling the work that should
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be done in all aspects of the field of synthetic biology, not only in biosensor construction.</p>
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<img id="UTK"alt="UTK" src="https://static.igem.org/mediawiki/2014/8/88/UTK.png" />
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<tr><td colspan="3" > <h3> Tips  </h3></td></tr>
 
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<p>We are currently working on providing teams with some easy to use design templates.
 
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
 
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
 
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
 
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
 
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
 
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<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
 
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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Revision as of 19:06, 15 July 2014


<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> UTK-2014

Abstract

  A major problem in modern biology is a lack of well understood microbial sensors and one limitation in metabolic engineering is an inability to control dynamic cell metabolism. Our project provides a solution to both problems with synthetic biology via a synthetic sensing scaffold. There are no standardized platforms for sensor characterization today, and as synthetic biology grows, so does the need to develop standards for the creation of genetic construct. The completion of this project provides a high throughput method of developing a library of microbial sensors, a mechanism for controlling cell metabolism based on environmental conditions, and a standardized platform for the design and characterization of synthetic sensing circuits.

     This project aims to introduce a “plug-and-play” method for sensing circuits, and to provide a protocol for characterizing novel sensors. A two-plasmid system, with a sensor and reporter, is employed in our standard. The sensor is created by generating a chimera between a sensing gene and a histidine kinase, which activates a transcriptional regulation protein. This protein controls the activity of the reporter. This upregulated reporter with a fluorescent protein will respond by fluorescing in the presence of our target due to sensor activity.

   A wide range of sensing domains can be used and characterized in this manner, making this system highly modular. This modularity leads to high throughput sensor characterization, which can be used with both known and unknown sensing domains. A successful biosensor circuit platform helps bridge the gap in today’s protocols, modeling the work that should be done in all aspects of the field of synthetic biology, not only in biosensor construction.


UTK

Retrieved from "http://2014.igem.org/Team:UTK-Knoxville"