Team:Genspace/Notebook/October

From 2014.igem.org

(Difference between revisions)
(Created page with "{{CSS/Main}} <html> <style> #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki sett...")
 
(6 intermediate revisions not shown)
Line 1: Line 1:
-
{{CSS/Main}}
+
{{Team:Genspace/GS14 Style}}
 +
{{Team:Genspace/GS14 menu}}
<html>
<html>
-
<style>
 
-
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
 
-
.wrapper {width:100%; padding:0; margin:0;}
 
-
.container {display: block; width:975px; margin:0 auto;}
 
-
.content {width: 100%;}
 
-
</style>
 
-
 
-
<!--  here ends the section that changes the default wiki template to a white full width background -->
 
-
 
-
 
<!-- beginning of your page -->
<!-- beginning of your page -->
<div class="wrapper"><div class="container"><div class="content">
<div class="wrapper"><div class="container"><div class="content">
-
<!-- menu -->
+
<!-- start of content -->
-
<table width="100%" align="center">
+
<h1>October Notebook</h1>
-
<tr heigth="15px"></tr>
+
-
<tr heigth="75px">  
+
 +
<h2>10.2.14 Plate with RFP, CFP, and OFP</h2>
 +
<h3>Background</h3>
 +
<p>On 10/2/14, we made a plate to make sure that the following samples would actually work:</p>
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
<ul>
-
<a href="https://2014.igem.org/Team:Genspace"style="color:#000000">Home </a> </td>
+
<li>Red 1</li>
 +
<li>Red 2</li>
 +
<li>Orange 1</li>
 +
<li>Orange 2</li>
 +
<li>Cyan 1</li>
 +
<li>Cyan 2</li>
 +
</ul>
 +
<p>Here are some useful links:</p>
 +
<p><a href="http://en.wikipedia.org/wiki/Green_fluorescent_protein" target="_blank">http://en.wikipedia.org/wiki/Green_fluorescent_protein</a></p>
 +
<p><a href="http://en.wikipedia.org/wiki/Fluorescence_spectroscopy" target="_blank">http://en.wikipedia.org/wiki/Fluorescence_spectroscopy</a></p>
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
<h3>Results</h3>
-
<a href="https://2014.igem.org/Team:Genspace/Team"style="color:#000000"> Team </a> </td>
+
-
<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
<img src="https://static.igem.org/mediawiki/2014/3/34/Results1.jpg" alt="Results" class="aligncenter" />
-
<a href="https://igem.org/Team.cgi?year=2014&team_name=Genspace"style="color:#000000"> Official Team Profile </a></td>
+
-
<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
<img src="https://static.igem.org/mediawiki/2014/1/10/Results2.jpg" alt="Results" class="aligncenter" />
-
<a href="https://2014.igem.org/Team:Genspace/Project"style="color:#000000"> Project</a></td>
+
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
<img src="https://static.igem.org/mediawiki/2014/2/2f/Results3.jpg" alt="Results" class="aligncenter" />
-
<a href="https://2014.igem.org/Team:Genspace/Parts"style="color:#000000"> Parts</a></td>
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
<p>Orange 1 does not work.  All of the others look good.  See the attached figures for more details:</p>
-
<a href="https://2014.igem.org/Team:Genspace/Modeling"style="color:#000000"> Modeling</a></td>
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
<ul>
-
<a href="https://2014.igem.org/Team:Genspace/Notebook"style="color:#000000"> Notebook</a></td>
+
<li>RFP_OFP_CFP_whiteBG2.jpg bottom of plate on a white background and image processed to reverse the writing</li>
 +
<li>RFP_OFP_CFP_UVoff2.jpg bottom of plate on UV light (while off) and image processed to reverse the writing</li>
 +
<li>RFP_OFP_CFP_UV2.jpg bottom of plate on UV light (while on) and image processed to reverse the writing</li>
 +
</ul>
 +
<p>Here's a chart to summarize how well the protein was produced.</p>
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
<div>
-
<a href="https://2014.igem.org/Team:Genspace/Safety"style=" color:#000000"> Safety </a></td>
+
<table><colgroup> <col width="22" /> <col width="189" /> <col width="158" /> <col width="158" /></colgroup>
-
 
+
<tbody>
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
<tr>
-
<a href="https://2014.igem.org/Team:Genspace/Attributions"style="color:#000000"> Attributions </a></td>
+
<td></td>
-
 
+
<td>
-
 
+
<p>CFP</p>
-
<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
+
</td>
 +
<td>
 +
<p>OFP</p>
 +
</td>
 +
<td>
 +
<p>RFP</p>
 +
</td>
</tr>
</tr>
 +
<tr>
 +
<td>
 +
<p>1</p>
 +
</td>
 +
<td>
 +
<p>good</p>
 +
</td>
 +
<td>
 +
<p>bad</p>
 +
</td>
 +
<td>
 +
<p>good</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td>
 +
<p>2</p>
 +
</td>
 +
<td>
 +
<p>good</p>
 +
</td>
 +
<td>
 +
<p>good</p>
 +
</td>
 +
<td>
 +
<p>good</p>
 +
</td>
 +
</tr>
 +
</tbody>
</table>
</table>
-
<!-- end of menu -->
+
</div>
 +
<p>The RFPs are obviously red, even under white light.  The CFPs have the strongest fluorescence under UV light.</p>
 +
<p>We should probably perform some tests to make sure that we are getting the wavelengths that we are expecting.</p>
 +
<p>There seems to be some stray colonies.  This may be from when I was pipetting.</p>
 +
<p>I took the plate out of the incubator at around 9PM on 10/3/14 (about 21 hours in the incubator).</p>
-
<!-- start of content -->
+
<h3>Conclusions</h3>
 +
<p>We should NOT use "Orange 1".  If we have time, we could investigate what went wrong.</p>
 +
<p>We should use the rest.</p>
 +
<p>The plates are in the refrigerator (the one furthest back in the lab) at the location indicated by the file plate location.jpg.</p>
-
<h1>October Notebook</h1>
+
<img src="https://static.igem.org/mediawiki/2014/4/4d/Plate-location.jpg" alt="Plate Location" class="aligncenter" />
 +
</div></div></div>
 +
</html>

Latest revision as of 02:31, 17 October 2014

October Notebook

10.2.14 Plate with RFP, CFP, and OFP

Background

On 10/2/14, we made a plate to make sure that the following samples would actually work:

  • Red 1
  • Red 2
  • Orange 1
  • Orange 2
  • Cyan 1
  • Cyan 2

Here are some useful links:

http://en.wikipedia.org/wiki/Green_fluorescent_protein

http://en.wikipedia.org/wiki/Fluorescence_spectroscopy

Results

Results Results Results

Orange 1 does not work.  All of the others look good.  See the attached figures for more details:

  • RFP_OFP_CFP_whiteBG2.jpg bottom of plate on a white background and image processed to reverse the writing
  • RFP_OFP_CFP_UVoff2.jpg bottom of plate on UV light (while off) and image processed to reverse the writing
  • RFP_OFP_CFP_UV2.jpg bottom of plate on UV light (while on) and image processed to reverse the writing

Here's a chart to summarize how well the protein was produced.

CFP

OFP

RFP

1

good

bad

good

2

good

good

good

The RFPs are obviously red, even under white light.  The CFPs have the strongest fluorescence under UV light.

We should probably perform some tests to make sure that we are getting the wavelengths that we are expecting.

There seems to be some stray colonies.  This may be from when I was pipetting.

I took the plate out of the incubator at around 9PM on 10/3/14 (about 21 hours in the incubator).

Conclusions

We should NOT use "Orange 1".  If we have time, we could investigate what went wrong.

We should use the rest.

The plates are in the refrigerator (the one furthest back in the lab) at the location indicated by the file plate location.jpg.

Plate Location

Retrieved from "http://2014.igem.org/Team:Genspace/Notebook/October"