Team:Colombia/Interlab

From 2014.igem.org

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<h3>Methods</h3>
<h3>Methods</h3>
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<h4>Method1</h4>
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<h4>Strains and culture</h4>
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All transformations were carried out in ''E. coli'' TOP10. This bacterium was cultures in LB (suplemented with the appropiate antibiotic for each case) at 37 °C.
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<h4>Method2</h4>
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<h4>Transformations and plasmidic DNA extration</h4>
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This process was carried out as  it is indicated in our [https://2014.igem.org/Team:Colombia/Protocols link protocols page]

Revision as of 02:37, 8 July 2014

Interlab Study

This year Colombia Team-iGEM is participating in Interlab Study! We are glad of share our results and measures. You can find them in the tabs below, you can find the specifications of our equipments and the methodology we used as well.

Methods And Validation

Methods

Text overview

Contents

Methods

Strains and culture

All transformations were carried out in E. coli TOP10. This bacterium was cultures in LB (suplemented with the appropiate antibiotic for each case) at 37 °C.

Transformations and plasmidic DNA extration

This process was carried out as it is indicated in our link protocols page


Validation

The following gels show respectively the plasmids with the devices and the restriction profiles for each of the tree devices digested with EcoRI and PstI. For restrictions we obtained two bands (the plasmid and the device) with the expected sizes in all cases.

GELES :)

You can check the size of each device with the next table:

Devices and their size
Name Backbone + device / bp Device / bp Linearized backbone / bp
BBa_I20260 5344 919 4425
BBa_J23101 + BBa_E0240(B0032-E0040-B0015) 2981 911 2070
BBa_J23115 + BBa_E0240 (B0032-E0040-B0015) 2981 911 2070

BBa_I20260 (J23101-B0032-E0040-B0015)

Measures

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Fluorescence microscopy

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Fluorometry

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BBa_J23101 + BBa_E0240 (B0032-E0040-B0015)

Measures

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Fluorescence microscopy

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Fluorometry

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Table2. Stress related cortisol levels form male, female and mean. (Kupfer, 1996)

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Figure 1. Scatter Plot of changes in Cortisol Levels (nmol/L) in Males, depending on the hour of the day.

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Figure 2. Scatter Plot of changes in Cortisol Levels (nmol/L) in Females, depending on the hour of the day.

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Figure 3. Scatter Plot of changes in Cortisol Levels (nmol/L) in the Mean between Males and Females, depending on the hour of the day with shaded area of threshold identified.


BBa_J23115 + BBa_E0240 (B0032-E0040-B0015)

Measures

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Fluorescence microscopy

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Fluorometry

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