Team:Colombia/Interlab

From 2014.igem.org

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{| border="2" align="center"
{| border="2" align="center"
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|+'''Primers used to construct XXXXXXX'''
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|+'''Devices and their size'''
| style="text-align: center;" |Name
| style="text-align: center;" |Name
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| style="text-align: center;" |Sequence
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| style="text-align: center;" |Backbone + device (bp)
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| style="text-align: center;" |Device (bp)
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| style="text-align: center;" |Linearized backbone (bp)
|-  
|-  
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| style="text-align: left;" |FW pqrr4      
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| style="text-align: left;" |BBa_I20260      
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| style="text-align: left;" |CTT GTC TAG ATT GCA TTT TGC AAA TTT TG
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| style="text-align: left;" |5344
 +
| style="text-align: left;" |919
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| style="text-align: left;" |4425
|-  
|-  
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| style="text-align: left;" |RV pqrr4
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| style="text-align: left;" |BBa_J23101 + BBa_E0240(B0032-E0040-B0015)     
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| style="text-align: left;" |GGT CAC TAG TCA ATC TAA TAA ATG CAG ATT
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| style="text-align: left;" |2981
 +
| style="text-align: left;" |911
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| style="text-align: left;" |2070
|-  
|-  
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| style="text-align: left;" |FW LuxOD47E
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| style="text-align: left;" |BBa_J23115 + BBa_E0240 (B0032-E0040-B0015) 
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| style="text-align: left;" |CAA TTC TAG AAA AGA GGA GAA ATA CTA GAT G
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| style="text-align: left;" |2981
-
 
+
| style="text-align: left;" |911
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|-
+
| style="text-align: left;" |2070
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| style="text-align: left;" |RV LuxOD47E
+
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| style="text-align: left;" |ATA TAC TAG TTG TGA CTC TAG TAG AGA
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|}
|}

Revision as of 02:33, 8 July 2014

Interlab Study

This year Colombia Team-iGEM is participating in Interlab Study! We are glad of share our results and measures. You can find them in the tabs below, you can find the specifications of our equipments and the methodology we used as well.

Methods And Validation

Methods

Text overview

Contents

Methods

Method1

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Method2

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Validation

The following gels show respectively the plasmids with the devices and the restriction profiles for each of the tree devices digested with EcoRI and PstI. For restrictions we obtained two bands (the plasmid and the device) with the expected sizes in all cases.

GELES :)

You can check the size of each device with the next table:

Devices and their size
Name Backbone + device (bp) Device (bp) Linearized backbone (bp)
BBa_I20260 5344 919 4425
BBa_J23101 + BBa_E0240(B0032-E0040-B0015) 2981 911 2070
BBa_J23115 + BBa_E0240 (B0032-E0040-B0015) 2981 911 2070

BBa_I20260 (J23101-B0032-E0040-B0015)

Measures

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Fluorescence microscopy

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Fluorometry

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BBa_J23101 + BBa_E0240 (B0032-E0040-B0015)

Measures

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Fluorescence microscopy

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Fluorometry

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Table2. Stress related cortisol levels form male, female and mean. (Kupfer, 1996)

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Figure 1. Scatter Plot of changes in Cortisol Levels (nmol/L) in Males, depending on the hour of the day.

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Figure 2. Scatter Plot of changes in Cortisol Levels (nmol/L) in Females, depending on the hour of the day.

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Figure 3. Scatter Plot of changes in Cortisol Levels (nmol/L) in the Mean between Males and Females, depending on the hour of the day with shaded area of threshold identified.


BBa_J23115 + BBa_E0240 (B0032-E0040-B0015)

Measures

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Fluorescence microscopy

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Fluorometry

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