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From 2014.igem.org

Revision as of 15:18, 16 October 2014 (view source) Manickchand (Talk | contribs) ← Older edit		Latest revision as of 15:18, 16 October 2014 (view source) Manickchand (Talk | contribs)
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Latest revision as of 15:18, 16 October 2014

Protocols

Cell lines, DNA synthesis and Plasmids Cryopreservation of bacteria Chemically competent cells preparation Preparation of plasmid DNA on a small scale Rapid transformation using Sorbitol Heat-Shock Transformation BioBrick Restriction Digest Biobrick ligation LB culture medium + Mercury chloride (ML) Cell growth quantification of mercury resistant Escherichia coli DH5α Quantification of Mercury bioaccumulated by metal binding protein (MBP) Quantification of Green Fluorescent Protein