Team:UFAM Brazil/Methods

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<tr><td><h1>Methods</h1></td></tr>
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<tr><td><h1>Protocols</h1></td></tr>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol1">1. Cell lines, DNA synthesis and Plasmids</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol1">Cell lines, DNA synthesis and Plasmids</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol2">2. Cryopreservation of bacteria</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol2">Cryopreservation of bacteria</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol3">3.Chemically competent cells preparation</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol3">Chemically competent cells preparation</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol4">4. Preparation of plasmid DNA on a small scale</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol4">Preparation of plasmid DNA on a small scale</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol5">5. Rapid transformation using Sorbitol</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol5">Rapid transformation using Sorbitol</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol6">6.Heat-Shock Transformation</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol6">Heat-Shock Transformation</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol7">7. .BioBrick Restriction Digest</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol7">BioBrick Restriction Digest</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol8">8. Biobrick ligation</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol8">Biobrick ligation</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol9">9. LB culture medium + Mercury chloride (ML)</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol9">LB culture medium + Mercury chloride (ML)</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol10">10. Cell growth quantification of mercury resistant  Escherichia coli DH5α</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol10">Cell growth quantification of mercury resistant  Escherichia coli DH5α</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol11">11. Quantification of Mercury bioaccumulated by metal binding protein (MBP)</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol11">Quantification of Mercury bioaccumulated by metal binding protein (MBP)</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol12">12. Quantification of Green Fluorescent Protein</a></p>
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<p><a href="https://2014.igem.org/Team:UFAM_Brazil/Protocol12">Quantification of Green Fluorescent Protein</a></p>
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Latest revision as of 15:18, 16 October 2014

Protocols

Cell lines, DNA synthesis and Plasmids

Cryopreservation of bacteria

Chemically competent cells preparation

Preparation of plasmid DNA on a small scale

Rapid transformation using Sorbitol

Heat-Shock Transformation

BioBrick Restriction Digest

Biobrick ligation

LB culture medium + Mercury chloride (ML)

Cell growth quantification of mercury resistant Escherichia coli DH5α

Quantification of Mercury bioaccumulated by metal binding protein (MBP)

Quantification of Green Fluorescent Protein